Study on the Visualization of Pigment in Haematococcus pluvialis by Raman Spectroscopy Technique

Abstract

As an ideal raw material for the production of astaxanthin, H . pluvialis was drawing attention during the last few years, there are some research topics initiated to find out the synthetic pathway of astaxanthin in H . pluvialis . In this study, confocal microscopic Raman technology was utilized to analyze the point-by-point mapping for H . pluvialis , and the visualization of pigment such as carotenoid and astaxanthin content were achieved. By comparing the Raman spectra of H . pluvialis and standard product of astaxanthin, and using the C = C stretching vibration of the Raman intensity as the main indicator for carotenoids, the visual spatial distribution for the carotenoids content was obtained. The MCR-ALS was applied to analyze the Raman data of H . pluvialis , and the information of astaxanthin was extracted to achieve real-time spatial distribution. The visualization of astaxanthin content shows that MCR-ALS is very effective for extracting the information of astaxanthin content from H . pluvialis . By exploring the spatial distribution of carotenoids and astaxanthin contents, analyzing the spatial distribution rules during its growth, Raman spectroscopy technology can be utilized to investigate the internal components of the pigment (ataxanthin, etc.) in H . pluvialis , which make it as an effective methodology to monitor the accumulation and changing mechanism of pigment content in microalgae.

Effects of dietary supplementation of natural astaxanthin from Haematococcus pluvialis on antioxidant capacity, lipid metabolism, and accumulation in the egg yolk of laying hens

“…Although it has the same chemical formula as ASTA, its physiological function, for example, the ability to scavenge free radicals, is far less than that of ASTA ( Capelli et al., 2013 ). Haematococcus pluvialis (a freshwater unicellular microalga) is one of the most effective organisms for the production of ASTA ( Shao et al., 2019 ). It has been shown that H. pluvialis can produce a large amount of ASTA under stress conditions, such as high temperature, light, and high salinity ( Sarada et al., 2002 ).…”

Structure elucidation of the novel carotenoid gemmatoxanthin from the photosynthetic complex of Gemmatimonas phototrophica AP64

“…S9). These three bands originate from (1) a rocking vibration of the molecule's methyl component (1015 and 1014 cm -1 ) 20 , (2) from C-C single-bond stretch vibrations (1158 cm -1 ) 21,22 and (3) C=C stretching vibrations (1525 and 1518 cm -1 ) of the molecule's backbone 20,22,23 . Other Raman bands elicited by the lasers were at 910 and 908 cm -1 and considered as C-C symmetric stretch 24 .…”

Raman Scattering-Based Biosensing: New Prospects and Opportunities

“…Over the past two decades, not many studies have been published covering nonlinear Raman techniques ( Figure 2 A), but there is undoubtedly interest in this area of research, which is evidenced by the successful application of CARS for the label-free visualization of lipid, protein, and nucleic acid distribution in cells [ 60 , 62 ]; pigments in living cells [ 63 ]; and skin tissues [ 64 ]. The CARS technique is of particular importance in the field of cancer cell identification and diagnosis research [ 65 , 66 , 67 ].…”