Study on the toxicity of phenolic and phenoxy herbicides using the submitochondrial particle assay

Argese, Emanuele; Bettiol, Cinzia; Marchetto, Davide; Vettori, S. De; Zambon, Alfonso; Miana, P.; Ghetti, Pier Francesco

doi:10.1016/j.tiv.2005.05.004

Cited by 35 publications

(20 citation statements)

References 27 publications

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“…The results of the two assays were compared quantitatively by means of linear regression analysis; a further comparison was made using literature data on fish acute toxicity (96-h LC50 for the fathead minnow Pimephales promelas, retrieved from the US EPA ECOTOX database, http://www.epa.gov/ ecotox) and EC50 values determined by a mitochondrial in vitro assay (SMP assay) in a previous study (Argese et al 2005). Unfortunately, other consistent datasets for the whole series of compounds were not found, both for terrestrial and aquatic organisms.…”

Section: Phenolic Herbicide Toxicitymentioning

confidence: 99%

“…With the purpose of a further validation of the seed germination/root elongation assay, toxicity was measured also by the Vibrio fischeri Microtox® test, a well-established bioassay based on bacterial bioluminescence inhibition; in addition, the acquired data were compared with those obtained in a previous study by a short-term in vitro assay based on mitochondrial respiratory functions (Argese et al 2005) and with literature data for fish acute toxicity from standard tests.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of phenolic herbicide toxicity and mode of action by different assays

Bettiol

Vettori

Minervini

et al. 2015

Environ Sci Pollut Res

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A phytotoxicity assay based on seed germination/root elongation has been optimized and used to evaluate the toxic effects of some phenolic herbicides. The method has been improved by investigating the influence of experimental conditions. Lepidium sativum was chosen as the most suitable species, showing high germinability, good repeatability of root length measurements, and low sensitivity to seed pretreatment. DMSO was the most appropriate solvent carrier for less water-soluble compounds. Three dinitrophenols and three hydroxybenzonitriles were tested: dinoterb, DNOC, 2,4-dinitrophenol, chloroxynil, bromoxynil, and ioxynil. Toxicity was also determined using the Vibrio fischeri Microtox® test, and a highly significant correlation was found between EC50 values obtained by the two assays. Dinoterb was the most toxic compound. The toxicity of hydroxybenzonitriles followed the order: ioxynil >bromoxynil >chloroxynil; L. sativum exhibited a slightly higher sensitivity than V. fischeri to these compounds. A QSAR analysis highlighted the importance of hydrophobic, electronic, and hydrogen-bonding interactions, in accordance with a mechanism of toxic action based on protonophoric uncoupling of oxidative phosphorylation. The results suggest that the seed germination/root elongation assay with L. sativum is a valid tool for the assessment of xenobiotic toxicity and can be recommended as part of a test battery.

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Section: Phenolic Herbicide Toxicitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Assessment of phenolic herbicide toxicity and mode of action by different assays

Bettiol

Vettori

Minervini

et al. 2015

Environ Sci Pollut Res

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show abstract

“…Mitochondria have been recognized as subcellular targets of many xenobiotic compounds; as a consequence, toxicity endpoints related to respiratory functions have been widely used in recent years to assess the adverse effects of various toxicants and to investigate the mechanisms of their action by means of suitable in vitro systems (Argese et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Biochemical and histological alterations of cellular metabolism from jerboa (Jaculus orientalis) by 2,4-dichlorophenoxyacetic acid: Effects on d-3-hydroxybutyrate dehydrogenase

Mountassif

Kabine

Mounchid

et al. 2008

Pesticide Biochemistry and Physiology

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“…[37] In addition, other factors such as acidity and alkalinity can affect the kinetic properties and toxicity of these compounds. Argese [38] showed that increased toxicity of Salvo et al…”

Section: Resultsmentioning

confidence: 99%

Toxicity assessment of 2,4-D and MCPA herbicides in primary culture of fish hepatic cells

Salvo

Malucelli

Silva

et al. 2015

Journal of Environmental Science and Health, Part B

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In this study, we used primary cultures of fish hepatic cells as a tool for evaluating the effects of environmental contamination. Primary hepatic cell cultures derived from the subtropical fish Metynnis roosevelti were exposed to different concentrations (0.275, 2.75 and 27.5 μg L(-1)) of the herbicides 2,4-dichlorophenoxyacetic acid (2,4-D) and 4-chloro-2-methylphenoxyacetic acid (MCPA). Cellular respiratory activity was evaluated by polarography using three substrates: 0.5 M glucose, 0.5 M succinate and 0.5 M α-ketoglutarate. Significant changes were observed in cellular oxygen consumption with 0.5 M α-ketoglutarate. Even at low concentrations, 2,4-D and MCPA were potent uncouplers of oxidative phosphorylation. Primary cultures of M. roosevelti liver cells may provide a useful tool for the evaluation of environmental contaminant effects. A review of regulations regarding permitted concentrations of these herbicides is needed.

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Study on the toxicity of phenolic and phenoxy herbicides using the submitochondrial particle assay

Cited by 35 publications

References 27 publications

Assessment of phenolic herbicide toxicity and mode of action by different assays

Assessment of phenolic herbicide toxicity and mode of action by different assays

Biochemical and histological alterations of cellular metabolism from jerboa (Jaculus orientalis) by 2,4-dichlorophenoxyacetic acid: Effects on d-3-hydroxybutyrate dehydrogenase

Toxicity assessment of 2,4-D and MCPA herbicides in primary culture of fish hepatic cells

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