Study on the toxicity of binary equitoxic mixtures of metals using the luminescent bacteria Vibrio fischeri as a biological target

Fulladosa, E.; Murat, J.C.; Villaescusa, Isabel

doi:10.1016/j.chemosphere.2004.08.007

Cited by 84 publications

(65 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Slightly higher toxicity of Pb in comparison to Cd observed in our study is in accordance with the findings of other authors [35]. Higher toxicity of Zn than Cd to V. fischeri, based on 15 min EC 50 values has already been reported [15]. The lack of sensitivity of Gram-negative bacteria towards cadmium (e.g.…”

Section: Sensitivity Of Bacterial Tests -Comparison To D Magnasupporting

confidence: 82%

“…V. fischeri has been used for more than 30 years in whole effluent toxicity testing (WET) [4], waste water treatment efficiency evaluation [5,6], surface water and sediment quality monitoring [7][8][9], ground water studies [10], risk assessment [11], and also as a model organism for fundamental studies on mode of action and toxicological pathways of various substances and mixtures [12][13][14][15][16]. Researchers have reported a good correlation of bioluminescence assay based on V. fischeri, the first and the most employed luminescent strain, with toxicity bioassays such as those on algae, crustacean, fishes, etc.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sensitivity of bacterial vs. acute Daphnia magna toxicity tests to metals

et al. 2009

View full text Add to dashboard Cite

The objectives of this study were to evaluate the sensitivity of two bacterial tests commonly used in metal toxicity screening — the Vibrio fischeri bioluminescence inhibition test and the Pseudomonas putida growth inhibition test — in comparison to the standard acute Daphnia magna test, and to estimate applicability of the selected methods to the toxicity testing of environmental samples. The D. magna acute test proved to be more sensitive to cadmium (Cd), zinc (Zn) and manganese (Mn) than the two bacterial assays, whereas P. putida seems to be the most sensitive species to lead (Pb). Manganese appears to be slightly toxic to D. magna and non-toxic to the two selected bacteria. This leads to the conclusion that even in regions with high background concentrations, manganese would not act as a confounding factor. Low sensitivity of V. fischeri to heavy metals questions its applicability as the first screening method in assessing various environmental samples. Therefore, it is not advisable to replace D. magna with bacterial species for metal screening tests. P. putida, V. fischeri and/or other bacterial tests should rather be applied in a complex battery of ecotoxicological tests, as their tolerance to heavy metals can unravel other potentially present toxic substances and mixtures, undetectable by metal-sensitive species.

show abstract

Section: Sensitivity Of Bacterial Tests -Comparison To D Magnasupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Sensitivity of bacterial vs. acute Daphnia magna toxicity tests to metals

et al. 2009

View full text Add to dashboard Cite

show abstract

“…A 20% level of negative effect is a significant alteration, considering that this work was completed in controlled laboratory experimental conditions [38]. For EC10, the lowest bound of its confidence interval was found to be close to a no-observed-effect concentration [39]; therefore, it can be considered a safe concentration.…”

Section: Discussionmentioning

confidence: 98%

Terrestrial ecotoxicity of short aliphatic protic ionic liquids

Peric

Martí

Sierra

et al. 2011

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

A study of the ecotoxicity of different short aliphatic protic ionic liquids (PILs) on terrestrial organisms was conducted. Tests performed within the present study include those assessing the effects of PILs on soil microbial functions (carbon and nitrogen mineralization) and terrestrial plants. The results show that the nominal lowest-observed-adverse-effect concentration (LOAEC) values were 5,000 mg/kg (dry soil) for the plant test in two species (Lolium perenne, Allium cepa), 1,000 mg/kg (dry soil) for the plant test in one species (Raphanus sativus), and 10,000 mg/kg (dry soil) for carbon and nitrogen microbial transformation tests (all concentrations are nominal). Most of the median effective concentration values (EC50) were above 1,000 mg/kg (dry soil). Based on the obtained results, these compounds can be described as nontoxic for soil microbiota and the analyzed plants, and potentially biodegradable in soils, as can be deduced from the respirometric experiment. The toxicity rises with the increase of complexity of the PILs molecule (branch and length of aliphatic chain) among the three PILs analyzed.

show abstract

“…When the EC25 concentrations (metal concentrations needed to produce a 25% decrease in fluorescence) of two metals are combined, they might produce a total toxicity equal to the sum of their EC25 values (AE2s). For example, mixtures of Hg with Cr, Hg with Zn, Cu with Co, Cu with Al, and Cr with Zn were all simply additive [22].…”

Section: Binary Combinations Of Heavy Metal Ionsmentioning

confidence: 99%

A DNA‐based assay for toxic chemicals in wastewater

Foreman

Phillips

Kanellis

et al. 2011

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Chemical toxicants, particularly metal ions, are a major contaminant in global waterways. Live-organism bioassays used to monitor chemical toxicants commonly involve measurements of activity or survival of a freshwater cladoceran (Ceriodaphnia dubia) or light emitted by the marine bacterium Vibrio fischeri, used in the commercial Microtox® bioassay. Here we describe a novel molecule-based assay system employing DNA as the chemical biosensor. Metals bind to DNA, causing structural changes that expel a bound (intercalated) fluorescent reporter dye. Analyses of test data using 48 wastewater samples potentially contaminated by metal ions show that the DNA-dye assay results correlate with those from C. dubia and Microtox bioassays. All three assays exhibit additive, antagonistic, and synergistic responses that cannot be predicted by knowing individual metal concentrations. Analyses of metals in these samples imply the presence of chemical toxicants other than metal ions. The DNA-dye assay is robust, has a 12-month shelf life, and is only slightly affected by sample pH in the range 4 to 9. The assay is completed in a matter of minutes, and its portability makes it well suited as a screening assay for use in the field. We conclude that the DNA-dye test is a surrogate bioassay suitable for screening chemical toxicity.

show abstract

Study on the toxicity of binary equitoxic mixtures of metals using the luminescent bacteria Vibrio fischeri as a biological target

Cited by 84 publications

References 17 publications

Sensitivity of bacterial vs. acute Daphnia magna toxicity tests to metals

Sensitivity of bacterial vs. acute Daphnia magna toxicity tests to metals

Terrestrial ecotoxicity of short aliphatic protic ionic liquids

A DNA‐based assay for toxic chemicals in wastewater

Contact Info

Product

Resources

About