Study on the structure of SF fiber mats electrospun with HFIP and FA and cells behavior

Zhang, Feng; Zuo, Baoqi; Bai, Liang

doi:10.1007/s10853-009-3800-5

Cited by 48 publications

(33 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The analysis was focused on the region ranging from 1800 to 1200 cm −1 , the most useful region for the analysis of SF amides. The spectrum of the aqueous SF sample shows the characteristic peaks of the random coil and α-helix conformation (water-soluble states) at 1653-1645 cm −1 (Amide I region) and ~1547 cm −1 (Amide II), respectively (Figure 2) (Zhang et al, 2009). The spectra of the SF particles (micro and nanoparticles) mainly shows the characteristic peaks of β-sheets structures or silk II (water insoluble) due to the MeOH treatment.…”

Section: Characterization Of the Micro And The Nanoparticlesmentioning

confidence: 99%

“…The spectra of the SF particles (micro and nanoparticles) mainly shows the characteristic peaks of β-sheets structures or silk II (water insoluble) due to the MeOH treatment. Amide regions I and II show a strong signal at ~1621 cm −1 and 1515 cm −1 , respectively, which are characteristic of β-sheets structures (Lozano-Pérez et al, 2014;Zhang et al, 2009). …”

Section: Characterization Of the Micro And The Nanoparticlesmentioning

confidence: 99%

See 1 more Smart Citation

Effect of aqueous and particulate silk fibroin in a rat model of experimental colitis

Rodríguez-Nogales

Lozano-Pérez

Aznar-Cervantes

et al. 2016

International Journal of Pharmaceutics

View full text Add to dashboard Cite

Section: Characterization Of the Micro And The Nanoparticlesmentioning

confidence: 99%

Section: Characterization Of the Micro And The Nanoparticlesmentioning

confidence: 99%

Effect of aqueous and particulate silk fibroin in a rat model of experimental colitis

Rodríguez-Nogales

Lozano-Pérez

Aznar-Cervantes

et al. 2016

International Journal of Pharmaceutics

View full text Add to dashboard Cite

“…Although fiber diameter increased with weight ratio of CA‐HAp/PCL, it was not believed to be a main factor on cell proliferation according to other published results. On silk fibroin electrospun sheets of fiber diameter as 0.3 and 2 µm, proliferation of primary normal human epidermal fibroblasts was not significantly different through 8 days based on MTT studies . When human osteosarcoma MG63 cells were seeded on collagen electrospun fibers of three diameters (50–200 nm, 200–500 nm, and 500–1000), MTT assay showed that cell viabilities were comparable on day 3 .…”

Section: Discussionmentioning

confidence: 97%

“…On silk fibroin electrospun sheets of fiber diameter as 0.3 and 2 µm, proliferation of primary normal human epidermal fibroblasts was not significantly different through 8 days based on MTT studies. [33] When human osteosarcoma MG63 cells were seeded on collagen electrospun fibers of three diameters (50-200 nm, 200-500 nm, and 500-1000), MTT assay showed that cell viabilities were comparable on day 3. [34] Among PLGA electrospun membranes of different fiber diameters 0.14, 0.78, and 3.6 µm, fibroblast cell densities were comparable though 14 days culture.…”

Section: Discussionmentioning

confidence: 99%

Development of Electrospun Composite Fibers in Multiscale Structure and Investigating the Performance on Proliferation and Osteogenic Differentiation of ADSCs

Liu

Shi

et al. 2018

Macro Materials & Eng

View full text Add to dashboard Cite

Electrospun composite membranes in multiscale structures are developed for bone tissue engineering. Aligned polycaprolactone (PCL) fibers entrapping CA‐HAp microparticles (containing CaCO3, hydroxyapatite, and casein in a hierarchical organization) are electrospun to find whether synergistic effects of fiber alignment and CA‐HAp microparticles on improving osteogenic differentiation can be obtained. CA‐HAp microparticles are in a spherical morphology of 1.42 ± 0.26 µm. Their presence increases fiber diameter and does not significantly affect fiber alignment. On all membranes, adipose derived stem cells (ADSCs) from humans spread very well. On a random group, cells distribute randomly and the presence of CA‐HAp microparticles facilitates cell proliferation, especially for the one at CA‐HAp/PCL 50 wt%; the one at CA‐HAp/PCL 20 wt% shows significantly much higher alkaline phosphatase (ALP) activity (112.0% higher) than the pure PCL membrane. On aligned samples, cells align along fibers and expression of ALP is enhanced. However, at the same composition (CA‐HAp/PCL 20 wt%), the random sample has much higher ALP activity than the aligned sample. The expressions of osteogenic marker genes are also evaluated. Combining the results and the applicability of membranes together, the random membrane at CA‐HAp/PCL 20 wt% is the best candidate for bone tissue engineering.

show abstract

“…SF is a natural and biocompatible polymer proven to possess excellent mechanical properties in comparison to all other natural polymers [19]. Additionally, SF has been previously electrospun to produce fibers with a wide range of diameters, water resistance, and cell compatibility [20][21][22]. SF electrospun scaffold applications include vascular, bone, nerve, skin, and wound healing [23][24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Using Electrospun Scaffolds to Promote Macrophage Phenotypic Modulation and Support Wound Healing

Hixon¹,

Dunn²,

Flores³

et al. 2017

Electrospinning

View full text Add to dashboard Cite

Abstract:The development of pressure ulcers in spinal cord injury patients is extremely common, often requiring extensive surgical procedures. Macrophages (MACs) play a crucial role in the innate immune system, contributing to wound healing and overall regeneration. MACs have been found to possess the potential to be activated by external factors from their M0 inactive state to an M1 proinflammatory or M2 regenerative state. This study conducted a comprehensive evaluation of MAC phenotype in response to electrospun scaffolds of varying material fiber/pore diameter, fiber stiffness, and +/− inclusion of platelet-rich plasma (PRP). Generally, it was found that the addition of PRP resulted in decreased pore size, where 5 silk fibroin (SF) had the stiffest fibers. Furthermore, PRP scaffolds demonstrated an increased production of VEGF and chemotaxis. The polycaprolactone (PCL) and SF scaffolds had the largest cell infiltration and proliferation. Overall, it was found that 5% SF had both ideal fiber and pore structure, allowing for cell infiltration further enhanced by the presence of PRP. Additionally, this scaffold led to a reasonable production of VEGF while still allowing fibroblast proliferation to occur. These results suggest that such a scaffold could provide an off-the-shelf product capable of modifying the local MAC response.

show abstract

Study on the structure of SF fiber mats electrospun with HFIP and FA and cells behavior

Cited by 48 publications

References 23 publications

Effect of aqueous and particulate silk fibroin in a rat model of experimental colitis

Effect of aqueous and particulate silk fibroin in a rat model of experimental colitis

Development of Electrospun Composite Fibers in Multiscale Structure and Investigating the Performance on Proliferation and Osteogenic Differentiation of ADSCs

Using Electrospun Scaffolds to Promote Macrophage Phenotypic Modulation and Support Wound Healing

Contact Info

Product

Resources

About