Study on the Reproductive Performance of Korean Native Cattle(Hanwoo) by Sires in Jeonbuk Province

Kwon, Deok-Ho; Son, S. K.; Oh, Jae-Don; Shin, Donghyun; Im, Seok Ki; Heo, Jae-Young; Choi, You-Lim

doi:10.14397/jals.2021.55.1.65

Cited by 2 publications

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“…Although the nature of genes that in uence economically important traits is not generally known, arti cial selective breeding in this way has been successful. Hanwoo cattle has less diversity and relatively xed genetic variation than other breeds of cattle [43][44][45] . Since Hanwoo breeds have relatively less genetic diversity than other small species, the characteristics of cells isolated from Hanwoo muscle tissue will also be relatively small.…”

Section: [Fig 6 Site] Discussionmentioning

confidence: 99%

Investigating Proliferation and Differentiation Capacities of Hanwoo Myosatellite Cells at Different Passages for Developing Cell-Cultured Meat

Park

et al. 2023

Preprint

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The aim of study was to investigate proliferation and differentiation capacities of Hanwoo myosatellite cells for the development of Hanwoo cell cultures. From P1 to P19, the number of live cells decreased and the cell size increased. It was confirmed that the PAX7 mRNA was higher in P3 than P6 and P9 (p < 0.05). The maximum differentiation score was measured from P1 to P12. The maximum differentiation score maintained high from P1 to P10. Immunostaining was performed for both P1 and P10 cells to investigate differentiation characteristics. And there were no significant differences in differentiation characteristics between P1 and P10 cells. MYOG mRNA was low, whereas C-FOSmRNA was high (p < 0.05) in the late passage. Myosin and Tom20 protein also showed low values in the late passage (p < 0.05). In conclusion, our results suggest that it is appropriate to use P1 to P10 for the production of cultured meat using Hanwoo muscle cells. If cell culture meat production is performed without differentiation, the passage range may increase further. These results provide basic essential data required for further development of Hanwoo cell cultures, which could provide a valuable source of protein for human populations in the future.

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Section: [Fig 6 Site] Discussionmentioning

confidence: 99%

Investigating Proliferation and Differentiation Capacities of Hanwoo Myosatellite Cells at Different Passages for Developing Cell-Cultured Meat

Park

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Although the nature of genes that influence economically important traits is not generally known, artificial selective breeding in this way has been successful. Hanwoo cattle has less diversity and relatively fixed genetic variation than other breeds of cattle 24 – 26 . Since Hanwoo breeds have relatively less genetic diversity than other small species, the characteristics of cells isolated from Hanwoo muscle tissue will also be relatively small.…”

Section: Introductionmentioning

confidence: 99%

Investigating proliferation and differentiation capacities of Hanwoo steer myosatellite cells at different passages for developing cell-cultured meat

Park,

et al. 2023

Sci Rep

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The aim of study was to investigate proliferation and differentiation capacities of Hanwoo myosatellite cells for the development of Hanwoo cell cultures. From P1 to P19, the number of live cells decreased and the cell size increased. It was confirmed that the PAX7 mRNA was higher in P3 than P6 and P9 (p < 0.05). The maximum differentiation score was measured from P1 to P12. The maximum differentiation score maintained high from P1 to P10. Immunostaining was performed for both P1 and P10 cells to investigate differentiation characteristics. And there were no significant differences in differentiation characteristics between P1 and P10 cells. MYOG mRNA was low, whereas C-FOS mRNA was high (p < 0.05) in the late passage. Myosin and Tom20 protein also showed low values in the late passage (p < 0.05). In conclusion, our results suggest that it is appropriate to use P1 to P10 for the production of cultured meat using Hanwoo muscle cells. If cell culture meat production is performed without differentiation, the passage range may increase further. These results provide basic essential data required for further development of Hanwoo cell cultures, which could provide a valuable source of protein for human populations in the future.

show abstract

Study on the Reproductive Performance of Korean Native Cattle(Hanwoo) by Sires in Jeonbuk Province

Cited by 2 publications

References 0 publications

Investigating Proliferation and Differentiation Capacities of Hanwoo Myosatellite Cells at Different Passages for Developing Cell-Cultured Meat

Investigating Proliferation and Differentiation Capacities of Hanwoo Myosatellite Cells at Different Passages for Developing Cell-Cultured Meat

Investigating proliferation and differentiation capacities of Hanwoo steer myosatellite cells at different passages for developing cell-cultured meat

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