Study on the relationships between polymorphisms of&lt;I&gt;CXCR2&lt;/I&gt;gene and milk quality and mastitis of dairy cow

Xu, Min; Ping, Fu-Qiang; Chen, Shiyi; Lai, Songjia; Liu, Yiping

doi:10.3724/sp.j.1005.2008.00463

Cited by 5 publications

(6 citation statements)

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“…In mastitic cows, CXCR2 mRNA expression is obviously higher in milk neutrophils compared with healthy ones, which has also been described in prior publications by Alhussien & Dang [45] and Alhussien et al [46]. Additionally, previous literature documents that the CXCR2 gene polymorphism is closely interrelated with bovine mastitis susceptibility or resistance [43,47]. However, whether CXCR2 gene polymorphism is related with clinical mastitis in sheep still needs to be further investigated and explored.…”

Section: Discussionsupporting

confidence: 56%

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Digital gene expression analyses of mammary glands from meat ewes naturally infected with clinical mastitis

Gao

Zhao

et al. 2019

R. Soc. open sci.

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Clinical mastitis in sheep has gravely restrained production performance for a long time. Knowledge of mechanisms of its pathogenesis and resistance in meat sheep mammary gland with clinical mastitis are not yet understood, especially for clinical mastitis caused by natural infection. In this work, RNA-sequencing was firstly used to screen the differentially expressed genes (DEGs) in clinical mastitic mammary tissues (CMMTs) when compared with healthy mammary tissues (HMTs) from meat sheep flocks. We identified 420 DEGs including 316 upregulated and 104 downregulated genes in CMMTs. Gene ontology annotation revealed these DEGs were mainly engaged in immune response and inflammation response. Pathway enrichment showed they were primarily enriched in pathways relevant to inflammation, immune response and metabolism. Alternative splicing analysis showed most common differential splicing genes in CMMTs and HMTs were implicated in immune response. Immunostaining for three immune response-related proteins encoded by DEGs were mainly observed in mammary epithelium from both CMMTs and HMTs, and their positive signals were more intensive in CMMTs than those in HMTs. These findings provide experimental basis and reference for further researching the molecular genetic mechanisms, particularly immune defence mechanisms, of sheep mammary gland during clinical mastitis.

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Section: Discussionsupporting

confidence: 56%

“…[46]. Additionally, previous literature documents that the CXCR2 gene polymorphism is closely interrelated with bovine mastitis susceptibility or resistance [43,47]. However, whether CXCR2 gene polymorphism is related with clinical mastitis in sheep still needs to be further investigated and explored.…”

Section: Discussionmentioning

confidence: 99%

Digital gene expression analyses of mammary glands from meat ewes naturally infected with clinical mastitis

Gao

Zhao

et al. 2019

R. Soc. open sci.

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“…It was found that SCC increases with increased parity that could be due to increased risk of infectious pathogens entering the udder [37] , [38] , [39] . Considering the SCC of the third-parity cows (SCC>400,000 cells/ml) significantly higher than the first-parity cows (SCC <150,000 cells/ml) [40] , we compared the differences of H3K27me3 modification and gene expression levels between the third-parity cows and the first-parity cows. As expected, the 25 decreased expression genes in the third parity cows were suppressed by the H3K27me3 modification, which were mainly located in the up2K-up1K region relative to TSS of the genes.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Bovine H3K27me3 Modifications and the Regulatory Effects on Genes Expressions in Peripheral Blood Lymphocytes

Zhang

et al. 2012

PLoS ONE

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BackgroundGene expression of lymphocytes was found to be influenced by histone methylation in mammals and trimethylation of lysine 27 on histone H3 (H3K27me3) normally represses genes expressions. Peripheral blood lymphocytes are the main source of somatic cells in the milk of dairy cows that vary frequently in response to the infection or injury of mammary gland and number of parities.MethodsThe genome-wide status of H3K27me3 modifications on blood lymphocytes in lactating Holsteins was performed via ChIP-Seq approach. Combined with digital gene expression (DGE) technique, the regulation effects of H3K27me3 on genes expressions were analyzed.ResultsThe ChIP-seq results showed that the peaks of H3K27me3 in cows lymphocytes were mainly enriched in the regions of up20K (∼50%), down20K (∼30%) and intron (∼28%) of the genes. Only ∼3% peaks were enriched in exon regions. Moreover, the highest H3K27me3 modification levels were mainly around the 2 Kb upstream of transcriptional start sites (TSS) of the genes. Using conjoint analysis with DGE data, we found that H3K27me3 marks tended to repress target genes expressions throughout whole gene regions especially acting on the promoter region. A total of 53 differential expressed genes were detected in third parity cows compared to first parity, and the 25 down-regulated genes (PSEN2 etc.) were negatively correlated with H3K27me3 levels on up2Kb to up1Kb of the genes, while the up-regulated genes were not showed in this relationship.ConclusionsThe first blueprint of bovine H3K27me3 marks that mediates gene silencing was generated. H3K27me3 plays its repressed role mainly in the regulatory region in bovine lymphocytes. The up2Kb to up1Kb region of the down-regulated genes in third parity cows could be potential target of H3K27me3 regulation. Further studies are warranted to understand the regulation mechanisms of H3K27me3 on somatic cell count increases and milk losses in latter parities of cows.

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“…Genetic polymorphism of investigated fragments of CXCR2 (exon 2) did not show significant association with the occurrence of mastitis. On the contrary, Xu et al (2008) reported five alleles of CXCR2 gene, based on the three SNPs (684, 777 and 861 bp), where three genotypes were associated with the incidence of mastitis. SNPs located within other regions of the bovine CXCR2 gene may also affect gene function during mastitis infections (Youngerman et al 2004 …”

Section: Association Studymentioning

confidence: 99%

“…Single-nucleotide polymorphisms (SNPs) have been identified in the bovine CXCR1 found to be associated with mastitis resistance (Leyva-Baca et al 2008). A few literatures (Youngerman et al 2004;Xu et al 2008) are available about association of mastitis with allelic variants of CXCR2 genes. However, CXCR2 gene still needs to be explored for SNPs associated with mastitis resistance in different cattle population.…”

Section: Introductionmentioning

confidence: 99%

Lack of association of mastitis with allelic variants ofCXCR2gene in Vrindavani cattle

Dige

Ahlawat

Bhushan

et al. 2013

Journal of Applied Animal Research

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The present study was aimed to identify single-nucleotide polymorphic (SNP) sites in coding regions of CXCR2 gene in Vrindavani cattle. A total of 95 animals were screened for CXCR2 gene locus. Single-strand conformation polymorphism (SSCP) analysis revealed polymorphic patterns, and a total of five distinct patterns were observed in the exon 2 of CXCR2 gene. Sequence analysis of different SSCP variants revealed the presence of five novel SNP at A214G, T340C, G344A, C856T and G1027A. No association of mastitis incidence was detected with different patterns of SSCP for both amplicons of CXCR2 exon 2.

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Study on the relationships between polymorphisms of<I>CXCR2</I>gene and milk quality and mastitis of dairy cow

Cited by 5 publications

References 5 publications

Digital gene expression analyses of mammary glands from meat ewes naturally infected with clinical mastitis

Digital gene expression analyses of mammary glands from meat ewes naturally infected with clinical mastitis

Genome-Wide Bovine H3K27me3 Modifications and the Regulatory Effects on Genes Expressions in Peripheral Blood Lymphocytes

Lack of association of mastitis with allelic variants ofCXCR2gene in Vrindavani cattle

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