Study on the Relationship Between Respiratory Distress Syndrome and SP-A1 (rs1059057) Gene Polymorphism in Mongolian Very Premature Infants

Abstract

Aim: To study the relationship between rs1059057 polymorphism of pulmonary surfactant protein A1 (SP-A1) and respiratory distress syndrome (RDS) in Mongolian very premature infants.Methods: Applying the strategy of case-control study, 120 Mongolian RDS very premature infants (58 males and 62 females) in the western part of Inner Mongolia were selected as the case group, and 120 subjects of non-RDS very premature infants (56 males and 64 females) with the same nationality, same sex and similar gestational age were used as the control group. The single nucleotide polymorphism (SNP) site rs1059057 of SP-A1 was genotyped using polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP).Results: Two genotypes, A/G and A/A, were detected at the SP-A1 rs1059057 locus in the western part of Inner Mongolia. In the case group, the frequencies of two genotypes were 53 and 47%, and the frequencies of A allele and G allele were 73 and 27%, respectively. In the control group, the frequencies of the two genotypes were 42 and 58%, and the frequencies of A allele and G allele were 79 and 21%, respectively. There was no significant difference in the genotype frequency of SP-A1 (rs1059057) locus between the case group and the control group (X 2 = 3.275, P > 0.05), and no significant difference in allele frequency between the case group and the control group (X 2 = 2.255, P > 0.05). Conclusion:The genotypes and allele frequencies of SP-A1 (rs1059057) locus were not associated with the incidence of RDS in Mongolian very premature infants in western Inner Mongolia.

Pathophysiological changes and injury markers for acute lung injury from blunt impact in infant rabbits

“…Regarding immature infant lungs, IHC of the right lung revealed a significant decrease in SP-A following acute impact injury, with gradual recovery observed between 24 and 72 h. Interestingly, we found that the SP-A changes in the left lung were similar to those seen in the injured lung. Moreover, in regions not directly impacted, bronchial SP-A changes mirrored those in lung tissue, showing a significant decrease immediately after injury and recovery within 24–72 h. Previous studies have revealed that post lung injury SP-A decreases ( 33 ), accompanying alveolar injury and secretion function, recruitment of neutrophils, and activation of macrophages that release a high number of inflammatory factors ( 34 ). This cascade exacerbates acute lung injury.…”

Comparative efficiency of differential diagnostic methods for the identification of BRAF V600E gene mutation in papillary thyroid cancer (Review)

“…PCR-SSCP can be used to detect sequence differences, but its sensitivity decreases with increasing DNA fragment lengths. SSCP has been previously used in cancer prognosis ( 69 , 70 ), asthma ( 71 ), blood group test ( 72 ), Gilbert syndrome ( 73 ), diabetes ( 74 ), respiratory distress syndrome ( 75 ), male varicocele infertility ( 76 ), gastric mucosa ( 77 ), traditional Chinese medicine ( 78 ), bacterial DNA detection ( 79 ) and identification of Trichomonas vaginalis ( 80 ).…”

Sensitivity and applications of the PCR Single-Strand Conformation Polymorphism method

“…Detection of somatic mutations in cancer, genes responsible for hereditary diseases, polymorphisms and other applications are described in detail at Kakavas et al [ 8 – 10 ]. Recent researches in the last 4 years shows dispersion of SSCP method in the above applications: on cancer prognosis [ 11 , 12 ], on asthma disease [ 13 ], on blood groups [ 14 ], on gilbert’s syndrome [ 15 ], on diabetes disease [ 16 ], on respiratory distress syndrome [ 17 ], on infertile men with varicocele [ 18 ], on gastric mucosa [ 19 ], on traditional Chinese medicine [ 20 ], detection of bacterial DNA [ 21 ], identification of trichomonas vaginalis [ 22 ].…”