Study on the preparation of collagen-modified silk fibroin films and their properties

Tang, Yin; Cao, Chuanbao; Ma, Xinzhi; Chen, Chen; Zhu, Hesun

doi:10.1088/1748-6041/1/4/010

Cited by 21 publications

(16 citation statements)

References 33 publications

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“…With the content of silk fibroin increase, the tensile stress, as well as the elastic modulus of the membranes decreased. However, the elongation at break of the membranes increased with the increase of the silk fibroin, which was consistent with the conclusion made by others . Specifically, the tensile strength of CS05 (1.14 ± 0.05 MPa) was nearly same to that of Col (1.11 ± 0.06 MPa), showing no significant difference ( p = 0.40).…”

Section: Resultssupporting

confidence: 91%

Improving the mechanical properties of collagen‐based membranes using silk fibroin for corneal tissue engineering

Long

Liu

et al. 2014

J Biomedical Materials Res

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Although collagen with outstanding biocompatibility has promising application in corneal tissue engineering, the mechanical properties of collagen-based scaffolds, especially suture retention strength, must be further improved to satisfy the requirements of clinical applications. This article describes a toughness reinforced collagen-based membrane using silk fibroin. The collagen-silk fibroin membranes based on collagen [silk fibroin (w/w) ratios of 100:5, 100:10, and 100:20] were prepared by using silk fibroin and cross-linking by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide. These membranes were analyzed by scanning electron microscopy and their optical property, and NaCl and tryptophan diffusivity had been tested. The water content was found to be dependent on the content of silk fibroin, and CS10 membrane (loading 10 wt % of silk fibroin) performed the optimal mechanical properties. Also the suture experiments have proved CS10 has high suture retention strength, which can be sutured in rabbit eyes integrally. Moreover, the composite membrane proved good biocompatibility for the proliferation of human corneal epithelial cells in vitro. Lamellar keratoplasty shows that CS10 membrane promoted complete epithelialization in 35 ± 5 days, and their transparency is restored quickly in the first month. Corneal rejection reaction, neovascularization, and keratoconus are not observed. The composite films show potential for use in the field of corneal tissue engineering.

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Section: Resultssupporting

confidence: 91%

Improving the mechanical properties of collagen‐based membranes using silk fibroin for corneal tissue engineering

Long

Liu

et al. 2014

J Biomedical Materials Res

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“…The molecular conformation of B. mori silk fibroin is characterized by β-sheet absorption peaks around 1630, 1530 and 1240 cm −1 , random coil conformation absorption peaks at 1650 or 1645, 1550 and 1230 cm −1 , and an α-helix absorption peak around 1655 cm −1 [15,22]. Tang and colleagues had previously reported that the intensity of peaks around 3300 cm -1 (data not shown here) fluctuate in response to hydrogen bonds [23]. …”

Section: Resultsmentioning

confidence: 79%

Preparation and characterization of silk fibroin as a biomaterial with potential for drug delivery

et al. 2012

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BackgroundDegummed silk fibroin from Bombyx mori (silkworm) has potential carrier capabilities for drug delivery in humans; however, the processing methods have yet to be comparatively analyzed to determine the differential effects on the silk protein properties, including crystalline structure and activity.MethodsIn this study, we treated degummed silk with four kinds of calcium-alcohol solutions, and performed secondary structure measurements and enzyme activity test to distinguish the differences between the regenerated fibroins and degummed silk fibroin.ResultsGel electrophoresis analysis revealed that Ca(NO3)2-methanol, Ca(NO3)2-ethanol, or CaCl2-methanol treatments produced more lower molecular weights of silk fibroin than CaCl2-ethanol. X-ray diffraction and Fourier-transform infrared spectroscopy showed that CaCl2-ethanol produced a crystalline structure with more silk I (α-form, type II β-turn), while the other treatments produced more silk II (β-form, anti-parallel β-pleated sheet). Solid-State 13C cross polarization and magic angle spinning-nuclear magnetic resonance measurements suggested that regenerated fibroins from CaCl2-ethanol were nearly identical to degummed silk fibroin, while the other treatments produced fibroins with significantly different chemical shifts. Finally, enzyme activity test indicated that silk fibroins from CaCl2-ethanol had higher activity when linked to a known chemotherapeutic drug, L-asparaginase, than the fibroins from other treatments.ConclusionsCollectively, these results suggest that the CaCl2-ethanol processing method produces silk fibroin with biomaterial properties that are appropriate for drug delivery.

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“…These disadvantages can be addressed by the use of low molecular weight heparins instead of unfractionated heparin [39, 41]. We used the low molecular weight heparin Tinzaparin to functionalize silk; no other previous study has used this fractionated heparin in combination with silk [22, 23, 25, 42]. …”

Section: Discussionmentioning

confidence: 99%

“…Previous reports have applied various functional coagulation tests to analyse heparin modified silk films or fibers (e.g., [22, 23, 25, 42]). All these studies reported improvement of the clotting parameters but did not examine the inflammatory response towards silk and its potential effect on the anticoagulant state of endothelial cells.…”

Section: Discussionmentioning

confidence: 99%

“…To date, a number of studies have evaluated the hemocompatibility of silk [19-21], silk alloys composed of silk and collagen [22, 23], silk and keratin [24], silk and chitosan [25], silk blends with either heparin [22] or ferulic acid [26], or silk modified chemically through sulfation [27, 28] or grafting with S-carboxymethyl keratin [24] or heparin [25]. We previously determined the impact of processing parameters on the hemocompatibility of pure silk films by using human whole blood and reference materials [29].…”

Section: Introductionmentioning

confidence: 99%

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Multifunctional silk–heparin biomaterials for vascular tissue engineering applications

et al. 2014

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Over the past 30 years, silk has been proposed for numerous biomedical applications that go beyond its traditional use as a suture material. Silk sutures are well tolerated in humans, but the use of silk for vascular engineering applications still requires extensive biocompatibility testing. Some studies have indicated a need to modify silk to yield a hemocompatible surface. This study examined the potential of low molecular weight heparin as a material for refining silk properties by acting as a carrier for vascular endothelial growth factor (VEGF) and improving silk hemocompatibility. Heparinized silk showed a controlled VEGF release over 6 days; the released VEGF was bioactive and supported the growth of human endothelial cells. Silk samples were then assessed using a humanized hemocompatibility system that employs whole blood and endothelial cells. The overall thrombogenic response for silk was very low and similar to the clinical reference material polytetrafluoroethylene. Despite an initial inflammatory response to silk, apparent as complement and leukocyte activation, the endothelium was maintained in a resting, anticoagulant state. The low thrombogenic response and the ability to control VEGF release support the further development of silk for vascular applications.

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Study on the preparation of collagen-modified silk fibroin films and their properties

Cited by 21 publications

References 33 publications

Improving the mechanical properties of collagen‐based membranes using silk fibroin for corneal tissue engineering

Improving the mechanical properties of collagen‐based membranes using silk fibroin for corneal tissue engineering

Preparation and characterization of silk fibroin as a biomaterial with potential for drug delivery

Multifunctional silk–heparin biomaterials for vascular tissue engineering applications

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