Study on The Potency of Methanol Extracts From Xanthosoma nigrum Stellfeld As Natural Anti Oxidant by Thiobarbituric Acid Method

Abstract

In this research Xanthosoma nigrum Stellfeld (the Purple yam) was selected as experimental material. This plant was collected from Rejang Lebong region, Bengkulu Province. Methanol extract 96% from stem of purple yam was studied its anti-oxidant activity in various concentrations with α-tocopherol (200 ppm) as standard of antioxidant. Antioxidant activity was determined using Thiobarbituric Acid (TBA) method. Linoleic acid was oxidized at 40 ºC for seven days with or without extract and the final product malondialdehyde (MDA) was reacted with thiostembituric acid to be of red colored complex (MDA-TBA) and was then measured by UV-VIS spectrophotometer at λ 532 nm. Stem extract of purple yam with concentration of 100 ppm, 150 ppm, 200 ppm and 300 ppm respectively had the inhibition of 19.32%, 21.85%, 29.47%, and 31.05%. α-Tocopherol as positive control which showed inhibition ability of 85.14% at 200 ppm. Based on the result obtained in this study, the stem's extract of Purpel yam plant showed that antioxidant activity was lower than α-tocopherol.

In Vitro Evaluation of Purple Sweet Potato Leaf Extract (Ipomoea batatas) as a Tyrosinase Inhibitor and Malondialdehyde Formation Inhibitor

“…The standard curve that has been determined becomes a reference for the linear equation for the determination of linoleic acid incubation and the MDA test from sweet potato leaf samples. The diene conjugation method for determining the incubation time of linoleic acid was carried out before testing the antioxidant activity of the samples by measuring the hydroperoxides which were measured every day to obtain the maximum absorbance value (Ratnawati et al, 2013). Linoleic acid incubation was carried out from day 0 to day 10 with the result that on day 6 the maximum absorbance value was obtained which then decreased on day 7.…”

Efficacy of Andrographis paniculata supplements induce a non-specific immune system against the pathogenicity of Aeromonas hydrophila infection in Indian major carp (Labeo rohita)

“…The constant practice of using antibiotics can lead to the advance of antibiotic-resistant pathogens; the presence of these chemicals, left as residues in the fish, is dangerous for human consumption also causing harmful bioaccumulation impacts (Hidayat et al 2018). Recently, herbal immunostimulants such as organic compounds from plants have been commonly used as an antibacterial and immunostimulant to control fish diseases (Cuesta et al 2004;Ratnawati et al 2013;Karina et al 2015;Hardi et al 2016a;Karina et al 2016).…”