Study on the molecular mechanisms of tetrandrine against pulmonary fibrosis based on network pharmacology, molecular docking and experimental verification

Preliminary Study on the Effect and Molecular Mechanism of Tetrandrine in Alleviating Pulmonary Inflammation and Fibrosis Induced by Silicon Dioxide

“…On the basis of a comprehensive analysis of the literature research results [ 31 , 41 , 42 , 43 ] and our previous work [ 33 ], we speculate that Tet may mitigate lung inflammation and fibrosis induced by SiO 2 in mice by regulating macrophage autophagy. Based on the related studies, ATG7, LC3B, and P62, which play important roles in autophagy regulation, were selected as the research focus, and systematically studied the effects of SiO 2 exposure and/or Tet intervention on these indexes, with a view to reveal whether Tet intervention may relieve lung inflammation and fibrosis induced by SiO 2 through the autophagy pathway.…”

“…Another study pointed out that Tet restores TGF-β1-induced autophagy flux impairment while enhancing the interaction between SQSTM1/P62 and MAP1LC3-II [ 32 ]. Additionally, our previous research indicated that Tet could target PDPK1 (PDK1), RAC1, KDR, PIK3CA, PTK2, and RPS6KB1 to exert anti-fibrotic effects [ 33 ], with further analysis showing that these molecules were closely related to autophagy. To sum up, we speculate that Tet may play an anti-inflammatory and fibrotic role by regulating autophagy in vivo.…”

An integrated strategy of UPLC-Q-TOF-MS analysis, network pharmacology, and molecular docking to explore the chemical constituents and mechanism of Zixue Powder against febrile seizures

“…Network pharmacology is an integrated approach that blends the disciplines of systems biology and bioinformatics and network science to investigate the underlying molecular mechanisms between drugs and therapeutic targets, which has been frequently used in the research of TCM [ 17 ]. Meanwhile, molecular docking can be used to screen active ingredients by predicting the binding affinity between ingredients and proteins, which is a method widely used in drug discovery [ 18 , 19 ].…”