Study on the method to avoid infusion‑site adverse events following chemotherapeutic treatment with epirubicin and fosaprepitant using immortalized human umbilical vein endothelial cells

Abstract

The combination of intravenous Proemend ® containing fosaprepitant meglumine, a prodrug for fosaprepitant (FAP), and Tween 80 and chemotherapy with anthracyclines, such as epirubicin (EPI), can cause infusion-site adverse events in clinical practice. In immortalized human umbilical vein endothelial (HUEhT-1) cells, the cytotoxic effects of FAP, EPI, diluted Proemend with culture medium and Tween 80 alone, and a combination of FAP and EPI, were evaluated using the WST-1 cell viability assay. FAP, EPI and diluted Proemend exhibited cytotoxicity in a concentration-dependent manner and marked synergic cytotoxicity was observed between FAP and EPI. The washing of the cell surface following incubation with diluted Proemend containing FAP and Tween-80 eliminated the synergic cytotoxicity of EPI applied thereafter. These results indicated that washing of the infusion-site vascular tissue following intravenous Proemend administration via intravenous tube flushing with an efficient amount of saline may reduce the infusion-site adverse events, which are caused by the combined use of FAP and EPI.

Intermittent hypoxia increased the expression of ESM1 and ICAM‐1 in vascular endothelial cells via the downregulation of microRNA‐181a1

“…The human vascular endothelial HUEhT‐1 cells, which were established from human umbilical vein endothelial cell line by electroporation of pIRES‐hTERT‐hygr, 20 , 21 , 22 and mouse vascular endothelial UV2 cells 23 , 24 , 25 were purchased from the Japanese Collection of Research Bioresources Cell Bank (Ibaraki, Japan) and RIKEN BioResorce Research Center, respectively. The HUEhT‐1 cells were grown in MCDB131 medium (Gibco) containing 10% (v/v) foetal calf serum (FCS), 10 mM L‐glutamine, 5 μg/mL heparin, 30 mg/L endothelial cell growth supplement (Corning, Corning, NY), 100 units/mL penicillin G (FUJIFILM Wako) and 100 μg/mL streptomycin (FUJIFILM Wako).…”