Study on the Mechanism of Lactobacillus Rhamnosus Probio-M9 Combined With Antibiotics on Staphylococcus Aureus Mastitis in Rat

Abstract

This study aims to investigate the potential anti-inflammatory mechanism of Lactobacillus rhamnosus Probio-M9 (Probio-M9) combined with antibiotics. Probio-M9, a probiotic strain previously isolated from the milk of healthy women. The Illumina Hiseq platform performs metagenomic sequencing on faeces samples at various time points; UPLC-Q-TOF/MS is used to detect a large number of endogenous metabolites in the serum of rats with mastitis induced by Staphylococcus aureus. The results show that the Probio-M9 combined with antibiotics increases beneficial intestinal bacteria (Lactobacillus murinus, Lactobacillus reuteri) and positively regulates metabolites (linoleic acid, glycerophospholipids, arachidonic acid, Sphingolipids. etc.), reduce inflammation in the body to play a protective role against mastitis. This research provides a new research method to relieve mastitis, and also provides a theoretical basis for the development and utilization of Probio-M9.