Study on the mechanism of Cortex Lycii on lung cancer based on network pharmacology combined with experimental validation

Guo, Zhen-Hui; Yin, Heng; Wu, Tong; Wu, Shaofeng; Liu, Lingyun; Zhang, Lei; He, Yanli; Zhang, Ren; Liu, Na

doi:10.1016/j.jep.2022.115280

Cited by 5 publications

(7 citation statements)

References 37 publications

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“…1F). Our previous report revealed that LRC did not suppress the proliferation of the NSCLC cell lines A549 and H1299 [15] . These results indicate that the inhibition of NSCLC progression in LLC-bearing mice was not due to the tumor-selective cytotoxicity of LRC and suggest that LRC could exert its anti-tumor effect through the immune response.…”

Section: Lrc Effectively Suppressed Tumor Growth In Vivomentioning

confidence: 93%

“…We kept a voucher specimen (LRC202008) of the LRC in our laboratory for future reference. We prepared and controlled the quality of LRC as we previously described [15]. In short, the dried LRC (2 000 × g) was ground into powder and soaked in 20 L of 95% ethanol (24 h, twice).…”

Section: Preparation For Extracts Of Lycii Radicis Cortexmentioning

confidence: 99%

“…Before use, the LRC solution was filtered with a 0.22 μm microporous filter membrane to remove insoluble matter. The production batch of LCR used in this study was the same as in our previous report [15] , which contained the HPLC (Shimadzu (Suzhou) Instruments Manufacturing Co., Ltd., Suzhou, China) analysis of flavonoids in LRC.…”

Section: Preparation For Extracts Of Lycii Radicis Cortexmentioning

confidence: 99%

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Lycii Radicis Cortex suppresses the growth of non-small cell lung cancer via enhancing the anti-tumor immunity

Yin,

Liu,

Zhao

et al. 2024

Food Science and Human Wellness

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Lycii Radicis Cortex (LRC) is a medicinal and food homologous plant with various pharmacological activities, including anti-tumor effects. This study explores the anti-tumor effect of LRC on non-small cell lung cancer (NSCLC) and its molecular mechanism using mice bearing Lewis lung carcinoma cells. LRC significantly suppressed the growth of NSCLC. Besides, RNA sequencing of mice tumors and hematoxylin & eosin and immunofluorescence staining revealed that LRC promoted the infiltration of T lymphocytes, specifically GZMB + CD8 + T lymphocytes, in tumor tissues. The Gene Set Enrichment Analysis of spleen RNA indicated that LRC up-regulated PD-1-downstream pathways, suggesting that LRC exerted its effects through the PD-L1/PD-1 pathway. Further experiments revealed that LRC interacted with PD-L1, blocking PD-L1/PD-1 binding and thus restoring the T cell killing activity on tumor cells. Together, these results support using LRC as healthy food to improve anti-tumor immunity in patients with NSCLC.

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Section: Lrc Effectively Suppressed Tumor Growth In Vivomentioning

confidence: 93%

Section: Preparation For Extracts Of Lycii Radicis Cortexmentioning

confidence: 99%

Section: Preparation For Extracts Of Lycii Radicis Cortexmentioning

confidence: 99%

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Lycii Radicis Cortex suppresses the growth of non-small cell lung cancer via enhancing the anti-tumor immunity

Yin,

Liu,

Zhao

et al. 2024

Food Science and Human Wellness

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“…TCM treats Yin de ciency heat (YDH) by focusing on two main approaches: nourishing Yin and suppressing internal heat 8 . Cortex Lycii is a popular medicine for treating night sweats induced by YDH, and it is the root bark of Lycium Chinense Mill 9 . The rst traditional Chinese medical book was published in 221 B.C., in which Shennong Ben Cao Jing described the effect of Cortex Lycii on suppressing internal heat.…”

Section: Introductionmentioning

confidence: 99%

Cortex Lycii attenuates night sweat in Yin-deficient rat by downregulating β 2 AR/cAMP signaling: A proteomic approach

Huang,

Hong,

Wang

et al. 2024

Preprint

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Purpose: To investigate the therapeutic effect of Cortex Lycii on the sweat secretion of YDH rats and its underlying mechanism. Methods: Male SD rats (N=36) were randomly assigned into Control, YDH, and DI groups (N=12 each group). YDH model rats were constructed by oral administration of thyroxin and reserpine. After two-weeks treatment of Cortex Lycii, the pharmacodynamics was assessed then the protein profile was obtained using a tandem mass tag proteomic approach. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analysis was used for bioinformatics analysis of differential expressed proteins (DEPs), critical DEPs were validated by Western blot. The receptor involved in the Cortex Lycii were confirmed by using β2AR receptor agonist (isoproterenol)- and M3R receptor agonist (pilocarpine)-induced sweat secretion in YDH rats. Results: Cortex Lycii attenuates sweat secretion in YDH rats, and down-regulated the expression of AQP5 and β2-receptor on paw pads of YDH rats. Proteomics analysis revealed 189 differential proteins between the model and control groups, of which 124 differential proteins were remarkably reversed by Cortex Lycii. The overlapping DEPs in the model and Cortex Lycii groups were mainly enriched in the β2AR/cAMP pathway. Moreover, Cortex Lycii considerably attenuated the agonistic effect of isoprenaline induced sweat secretion. Conclusion: This study demonstrated the effect of Cortex Lycii on sweat secretion in Yin-deficient rats. The mechanism on the ground may refer to the inhibition of β2AR/cAMP, followed by the suppression of the downstream PKA/ezrin/CFTR signaling pathway to reduce ion exchange and the inhibition of IP3/Ca2+/AQP5. The finding may provide new research on the mechanism of night sweats in TCM and their amelioration by Cortex Lycii.

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“…Furthermore, natural compounds derived from herbs are considered suitable drugs for cancer therapies due to their advantages of multi-targeting, efficacy, and low toxicity ( Sayanta et al, 2019 ). CM, derived from the Morus alba L. (a mulberry tree), is commonly used as TCM for the alleviation of various diseases, such as cough ( Yang et al, 2014 ), diabetes ( Wang et al, 2020 ), inflammation ( Carson et al, 2002 ), and cancers ( Nam et al, 2002 ; Park et al, 2012 ; Guo et al, 2022 ). Modern pharmacological studies have revealed that the anti-lung cancer effect of CM extracts can induce cell apoptosis by inhibiting microtubule assembly ( Carson et al, 2002 ).…”

Section: Introductionmentioning

confidence: 99%

Cortex Mori extracts induce apoptosis and inhibit tumor invasion via blockage of the PI3K/AKT signaling in melanoma cells

Zhang²,

Pan³

et al. 2022

Front. Pharmacol.

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Melanoma, the most aggressive and deadliest form of skin cancer, has attracted increased attention due to its increasing incidence worldwide. The Cortex Mori (CM) has long been used as a classical traditional Chinese medicine (TCM) to treat various diseases, including cancer. The bioactive components and underlying mechanisms, however, remain largely unknown. The current study aims to investigate the anti-melanoma effects of CM and potential mechanisms through combined network pharmacology and bioinformatic analyses, and validated by in vitro and in vivo experiments. We report here that CM has anti-melanoma activity both in vitro and in vivo. Furthermore, 25 bioactive compounds in CM were found to share 142 melanoma targets, and network pharmacology and enrichment analyses suggested that CM inhibits melanoma through multiple biological processes and signaling pathways, particularly the PI3K-AKT signaling inhibition and activation of apoptotic pathways, which were further confirmed by biochemical and histological examinations. Finally, partial CM-derived bioactive compounds were found to show anti-melanoma effects, validating the anti-melanoma potential of bioactive ingredients of CM. Taken together, these results reveal bioactive components and mechanisms of CM in inhibiting melanoma, providing them as potential anti-cancer natural products for the treatment of melanoma.

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Study on the mechanism of Cortex Lycii on lung cancer based on network pharmacology combined with experimental validation

Cited by 5 publications

References 37 publications

Lycii Radicis Cortex suppresses the growth of non-small cell lung cancer via enhancing the anti-tumor immunity

Lycii Radicis Cortex suppresses the growth of non-small cell lung cancer via enhancing the anti-tumor immunity

Cortex Lycii attenuates night sweat in Yin-deficient rat by downregulating β 2 AR/cAMP signaling: A proteomic approach

Cortex Mori extracts induce apoptosis and inhibit tumor invasion via blockage of the PI3K/AKT signaling in melanoma cells

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