Study on the mechanism of SALL4 down-regulation in promoting the invasion and migration of oral squamous cell carcinoma and influencing the survival and prognosis of patients

Abstract

Background: To investigate the identification of spalt-like transcription factor 4 (SALL4) in oral squamous cell carcinoma (OSCC).Methods: Recombinant cells loaded with miRNA expression cells were used to transform Tca8113 cells. Simple Tca8113 cells were used as the control group. We detected SALL4 messenger RNA (mRNA) before and after transfection by reverse transcription polymerase chain reaction (RT-PCR) and protein immunoblotting (western blot) A and protein expression. A dual luciferase reporter system was used to verify the targeted regulation of SALL4 and identify miRNA-S to test the effect of miRNA related to SALL4 regulation on the invasion and metastatic ability of Tca8113 cells. Results:The expression of SALL4 mRNA in Tca8113 cells was higher than that in the downregulated and control groups, respectively (P<0.05); there was no difference in Tca8113 cells between the upregulated and downregulated groups (P>0.05). Dual luciferase reporter system showed that the identified miRNA was miRNA-S; there were no differences in migration and invasion of Tca8113 cells between the up-and downregulated groups (P>0.05). Conclusions:In human OSCC, SALL4 regulation-related miRNAs are poorly expressed and can inhibit the invasion and metastasis of tumor cells, which is expected to become a new therapeutic target for OSCC.

Potential application of human SALL4 on oral squamous cell carcinoma

“…In human bone marrow, SALL4 expression is only linked to CD34 + HSCs and decreases after differentiation. In conclusion, the expression of SALL4 gene regulates the maintenance and proliferation of HSC in short and long term ( 2 , 6 ).…”