Study on the Joint Toxicity of BPZ, BPS, BPC and BPF to Zebrafish

Han, Ying; Fei, Yumeng; Wang, Mingxin; Xue, Yingang; Chen, Hui; Liu, Yuxuan

doi:10.3390/molecules26144180

Cited by 16 publications

(7 citation statements)

References 27 publications

(26 reference statements)

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“…According to the authors’ previous experimental results, the semi-lethal concentrations of BPC and BPZ were 2.76 mg/L and 2.57 mg/L, respectively, and the experimental exposure concentration was 1/100 of the semi-lethal concentration [ 17 ]. In this paper, DMSO was selected as the cosolvent, and the exposure concentrations of BPC and BPZ were 0.02 mg/L.…”

Section: Methodsmentioning

confidence: 99%

Effects of BPZ and BPC on Oxidative Stress of Zebrafish under Different pH Conditions

et al. 2022

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To further understand the toxic effects of bisphenol Z (BPZ) and bisphenol C (BPC) on aquatic organisms, zebrafish (Danio rerio) were exposed to 0.02 mg/L BPZ and BPC mixed solution in the laboratory for 28 days. The impacts of BPZ and BPC on the activity of the antioxidant enzymes, expression of antioxidant genes, and estrogen receptor genes in zebrafish under different pH conditions were studied. The changes of glutathione peroxidase (GSH-Px), reduced glutathione (GSH), total superoxide dismutase (T-SOD), catalase (POD), and malondialdehyde (MDA) in the zebrafish were detected by spectrophotometry. The mRNA relative expression levels of CAT, GSH, SOD, ERa, and ERb1 in the experimental group were determined by fluorescence quantitative PCR. The results showed that SOD activity and MDA content were inhibited under different pH conditions, and the activities of GSH, GSH-Px, and POD were induced. The activities of POD and GSH induced in the neutral environment were stronger than those in an acidic and alkaline environment. The mRNA relative expression levels of SOD and GSH were consistent with the activities of SOD and GSH. The mRNA relative expression levels of CAT were induced more strongly in the neutral environment than in acidic and alkaline conditions, the mRNA relative expression levels of ERa were induced most weakly in a neutral environment, and the mRNA relative expression levels of ERb1 were inhibited the most in a neutral environment.

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Section: Methodsmentioning

confidence: 99%

Effects of BPZ and BPC on Oxidative Stress of Zebrafish under Different pH Conditions

et al. 2022

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“…According to Chemicals—Fish, prolonged toxicity (GB/T 21808, 2008), the subacute toxicity of zebrafish exposed to four separate BPs was measured by an experiment of 13 days. On the basis of the acute toxicity of four bisphenol analogs to zebrafish, which has been studied [ 27 ], three concentration groups were set up, which were 1/10 LC 50 , 1/50 LC 50 , and 1/100 LC 50 , respectively. The specific concentrations are shown in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

Effects of BPZ, BPC, BPF, and BPS Exposure on Adult Zebrafish (Danio rerio): Accumulation, Oxidative Stress, and Gene Expression

Han

Liu

Wang

et al. 2022

IJERPH

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As substitutes for bisphenol A (BPA), bisphenol analogs (BPs) have been found to cause endocrine disorders and induce toxic effects. The objective of this study was to evaluate the bioaccumulation and subacute toxicity of bisphenol Z (BPZ), bisphenol C (BPC), bisphenol F (BPF), and bisphenol S (BPS) to zebrafish. Five-month-old zebrafish were exposed to 1/100 LC50, 1/50 LC50, and 1/10 LC50 of BPZ, BPC, BPF, and BPS for 13 days, respectively. Bioaccumulation, oxidative stress, and related mRNA expression in zebrafish tissues were measured on days 1, 7, and 13. After exposure, the four kinds of BPs all resulted in the accumulation of concentration and lipid peroxidation in zebrafish tissues to varying degrees. BPZ and BPC had the highest bioaccumulation level and had the greatest influence on malonic dialdehyde (MDA). In addition, the enzyme activities of superoxide dismutase (SOD), peroxidase (POD), glutathione peroxidase (GSH-PX), and the content of glutathione (GSH) in zebrafish tissues were also affected at different levels. However, the enzyme activities of SOD and POD were inactivated in zebrafish exposed to a high concentration of BPC. Further studies showed that BPs exposure down-regulated the transcription level of sod but up-regulated the relative expression levels of cat and gpx. The mRNA relative expression level of erα was not significantly changed, while the mRNA relative expression level of erβ1 was significantly down-regulated except under BPS exposure. These results indicate that BPZ, BPC, and BPF significantly affect the expression level of the estrogen receptor (ER) in zebrafish tissues. Overall, the results suggest that exposure to waterborne BPs can cause severe oxidative stress and tissue damage in adult zebrafish that is not sufficient to kill them after 13 days of waterborne exposure. The toxicity of BPs to organisms, therefore, should be further analyzed and evaluated.

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“…by assessment factors (AF) ranging from 10 to 1000 (Liu et al, 2020a;Yan et al, 2013). The LC50 values obtained from prior experiments were used as calculating data in this study, as reported by Han et al (2021). An RQ < 0.01, 0.01 < RQ < 1, and RQ > 1 indicated low, medium, and high ecological risk, respectively (Liu et al, 2020b).…”

Section: Parameter Measurement and Statistical Analysismentioning

confidence: 99%

Exposure, distribution, and ecological risk of four new bisphenol analogs in the typical lake region of Taihu Lake

Han

Liu

Rong

et al. 2023

Preprint

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The development of the industrial economy in Taihu Lake has led to increased levels of bisphenol compounds (BPs), which are difficult to degrade and highly toxic, making them a research focus. This study aimed to determine the occurrence, sources, and ecological risks of bisphenol Z (BPZ), bisphenol C (BPC), bisphenol F (BPF), and bisphenol S (BPS). Surface water and sediment samples were collected and analyzed, revealing average BPs concentrations of 1227 ng/L and 11 ng/g (dry weight), respectively. BPS had the highest detection rate among the four BPs, followed by BPF. Pollutant levels were found to increase upstream and decrease downstream. In wild fish, the pollutants were found to be concentrated in the muscle, gill, liver, kidney, and brain tissues, indicating short-term exposure. Correlation and stable isotope analyses showed that BPF and BPS had similar origins, and sediments were found to be the primary source of the pollutants. Ecological and human health risk assessments showed low-risk levels, but the risks are still increasing and require attention. This study provides valuable information for the management and control of BP pollution in Taihu Lake.

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Study on the Joint Toxicity of BPZ, BPS, BPC and BPF to Zebrafish

Cited by 16 publications

References 27 publications

Effects of BPZ and BPC on Oxidative Stress of Zebrafish under Different pH Conditions

Effects of BPZ and BPC on Oxidative Stress of Zebrafish under Different pH Conditions

Effects of BPZ, BPC, BPF, and BPS Exposure on Adult Zebrafish (Danio rerio): Accumulation, Oxidative Stress, and Gene Expression

Exposure, distribution, and ecological risk of four new bisphenol analogs in the typical lake region of Taihu Lake

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