Study on the Interaction of Colloidal Gold with Taq DNA Polymerase

Zhu, Hongping; Mi, Lijuan; Chen, Shi-Mou; Wang, Wenfeng; Yao, Si-Dea

doi:10.1002/cjoc.200790229

Cited by 8 publications

(2 citation statements)

References 31 publications

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“…Other reports also confirmed the presence of interactions between nanoparticles and DNA polymerases. 30,[58][59][60] Lin's group proposed an alternative mechanism that was associated the physical property of AuNPs. They proposed that heat conductivity of AuNPs played an important role in increasing the thermal cycling efficiency of the PCR reaction, hence the required reaction time could be dramatically shortened.…”

Section: Proposed Mechanisms For Nanopcrmentioning

confidence: 99%

Genetic analysis with nanoPCR

Pan

Huang

et al. 2012

Integr. Biol.

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Polymerase chain reaction (PCR) has become a standard and important molecular biological technique with numerous applications in genetic analysis, forensics and in vitro diagnostics. Since its invention in the 1980s, there has been dramatic performance improvement arising from long-lasting efforts to optimize amplification conditions in both academic studies and commercial applications. More recently, a range of nanometer-sized materials including metal nanoparticles, semiconductor quantum dots, carbon nanomaterials and polymer nanoparticles, have shown unique effects in tuning amplification processes of PCR. It is proposed that these artificial nanomaterials mimic protein components in the natural DNA replication machinery in vivo. These so-called nanomaterials-assisted PCR (nanoPCR) strategies shed new light on powerful PCR with unprecedented sensitivity, selectivity and extension rate. In this review, we aim to summarize recent progress in this direction and discuss possible mechanisms for such performance improvement and potential applications in genetic analysis (particularly gene typing and haplotyping) and diagnostics.

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Section: Proposed Mechanisms For Nanopcrmentioning

confidence: 99%

Genetic analysis with nanoPCR

Pan

Huang

et al. 2012

Integr. Biol.

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“…Several mechanisms have been proposed to account for the observed effects. We initially proposed that AuNPs realized their functions via selective binding to single‐stranded DNA, resembling single‐stranded binding (SSB) protein,23 while subsequent studies suggested that the interactions between AuNPs and polymerases played an important role as well 25–27. We thus propose that AuNPs possess multiple functions in complicated and diverse PCR systems, but one of these functions may predominate in a specific system.…”

Section: Introductionmentioning

confidence: 99%

Modulation of DNA Polymerases with Gold Nanoparticles and their Applications in Hot‐Start PCR

Wen

Pan

et al. 2009

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A new gold-nanoparticle (AuNP)-based strategy to dynamically modulate the activity of DNA polymerases and realize a hot-start (HS)-like effect in the polymerase chain reaction (PCR) is reported, which effectively prevents unwanted nonspecific amplification and improves the performance of PCRs. A high-fidelity Pfu DNA polymerase is employed as the model system. Interestingly, AuNPs inactivate the polymerase activity of Pfu at low temperature, thus resembling an antibody-based HS PCR. This inhibition effect of AuNPs is demonstrated for the preamplification polymerization activity of the PCR, which largely suppresses nonspecific amplification at temperatures between 30 and 60 degrees C and leads to highly specific and sensitive PCR amplification with Pfu. Significantly, the fidelity of Pfu is not sacrificed in the presence of AuNPs. Therefore, this AuNP-based HS strategy provides a straightforward and potentially versatile approach to realize high-performance PCR amplification.

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Synthesis, Structure and Growth Mechanism of Size and Shape Tunable Au/Ag Bimetallic Nanoparticles

Zheng

Shen

et al. 2009

Chin. J. Chem.

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By simply adding ascorbic acid in advance of AgNO 3 , the size and shape controllable Au/Ag bimetallic nanoparticles (NP) were prepared in the traditional Au growth solution free of seed at room temperature. The size distribution of NP is well uniform with ca. 10%-15% standard deviation in diameter. By changing CTAB concentration, the size and shape of NPs are tunable. After researching the surface-enhanced Raman spectroscopy (SERS) behavior of the prepared NPs, an enhancement factor varied from 4.3×10 4 to 1.1×10 5 was obtained for the NP centered at ca. (64±8) nm. Electrochemical cyclic voltammetric results revealed that the so formed nanoparticles were Au riched Au/Ag bimetallic NP, and this formation might be due to the disproportionation reaction of Au ＋ prompted by Ag ＋ and the under potential deposition process of Ag ＋ on Au.

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Study on the Interaction of Colloidal Gold with Taq DNA Polymerase

Cited by 8 publications

References 31 publications

Genetic analysis with nanoPCR

Genetic analysis with nanoPCR

Modulation of DNA Polymerases with Gold Nanoparticles and their Applications in Hot‐Start PCR

Synthesis, Structure and Growth Mechanism of Size and Shape Tunable Au/Ag Bimetallic Nanoparticles

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