Study on the interaction of antidiabetic drug Pioglitazone with calf thymus DNA using spectroscopic techniques

Qumaizi, Khalid I. Al; Anwer, Razique; Ahmad, Naeem; Alosaimi, Saleh M; Fatma, Tasneem

doi:10.1002/jmr.2735

Cited by 18 publications

(9 citation statements)

References 51 publications

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“…In a typical experiment, dye‐DNA complex was formed which gave a significant fluorescence signal at respective emission maxima. Aloe active compound was then added to the complex gradually with increasing concentration, and the changes in emission maxima were studied at 298.15 K . Drug that binds to DNA would be able to compete and displace the dyes at their respective binding site leading to fluorescence quenching of the DNA‐dye complexes.…”

Section: Methodsmentioning

confidence: 99%

Interaction of aloe active compounds with calf thymus DNA

Das

Kumar

Dutta

2019

J of Molecular Recognition

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Natural anthraquinone compounds have emerged as potent anticancer chemotherapeutic agents because of their promising DNA-binding properties. Aloe vera is among one of the very well-known medicinal plants, and the anthraquinone derivatives like aloe emodin (ALM), aloins (ALN), and aloe emodin-8-glucoside (ALMG) are known to have immense biological activities. Here, we have used biophysical methods to elu-

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Section: Methodsmentioning

confidence: 99%

Interaction of aloe active compounds with calf thymus DNA

Das

Kumar

Dutta

2019

J of Molecular Recognition

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“…The above dependencies were analyzed in terms of the simplified competition model (Eqs. [2][3][4][5][6][7][8][9][10][11][12][13] to calculate the association constants of the drug ( drug K ) binding to the DNA in the presence of AK3-5. The calculated drug K values were equal to 5.4×10 4 M -1 and 3.9×10 5 M -1 for V7 and V9, respectively.…”

Section: Eejp 3 (2019)mentioning

confidence: 99%

“…Due to advantages of rapidity and sensitivity, the fluorescence drug displacement assay seems to be an especially promising for the investigation of mechanisms underlying the interaction between nucleic acids and drugs. This method is based on a strong enhancement in the dye emission upon its binding to DNA or RNA, followed by the fluorescence drop upon the dye displacement by the drug [4][5][6][7]. Among a variety of commercially available dyes, ethidium bromide is the most widely used in the displacement experiments [7,8].…”

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confidence: 99%

Competitive Binding of Novel Cyanine Dye AK3-5 and Europium Coordination Complexes to DNA

Zhytniakivska

Zabrudska

Tarabara

et al. 2019

EEJP

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The present study was undertaken to assess the applicability of the novel trimethine cyanine dye AK3-5 as a competitive ligand for the antitumor agents, Eu(III) coordination complexes (EC), in the DNA-containing systems, using the displacement assay as an analytical instrument. The analysis of fluorescence spectra revealed a strong association of AK3-5 with nucleic acids, with the strength of interaction being higher for the double stranded DNA, compared to the single-stranded RNA. The binding parameters of the cyanine dye have been determined in terms of the McGhee & von Hippel neighbouring site-exclusion model and a classical Langmuir model. The AK3-5 association constant in the presence of DNA was found to be equal to 5.1×104 M-1, which is consistent to those of the well-known DNA intercalators. In turn, the binding of the cyanine to the RNA was characterized by a significantly lower association constant ( ~ 3.4×103 M-1) indicating either the external or “partially intercalated” binding mode. The addition of the europium complexes to the AK3-5-DNA system was followed by the fluorescence intensity decrease, with a magnitude of this effect being dependent on the EC structure. The observed fluorescence decrease of AK3-5 in the presence of europium complexes V7 and V9 points to the competition between the cyanine dye and antitumor drugs for the DNA binding sites. The dependencies of the AK3-5-DNA fluorescence intensity decrease vs. europium complex concentration were analyzed in terms of the Langmuir adsorption model, giving the values of the drug association constant equal to 5.4×104 M-1and 3.9×105 M-1 for the europium complexes V7 and V9, respectively. A more pronounced decrease of the AK3-5 fluorescence in the presence of V5 and V10 was interpreted in terms of the drug-induced quenching of the dye fluorescence, accompanying the competition between AK3-5 and Eu(III) complexes for the DNA binding sites. Cumulatively, the results presented here strongly suggest that AK3-5 can be effectively used in the nucleic acid studies and in the dye-drug displacement assays.

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“…Reactive oxygen species may also get generated because of these interactions in the DNA vicinity leading to its damage [8,12]. Hence, the DNA-drug binding studies are valuable to understand the binding action mechanisms to DNA [13,14]. ctDNA is preferred in these studies because of its ease in availability, structural similarities with mammalian DNA and cost effectiveness.…”

Section: Introductionmentioning

confidence: 99%

Interaction of an abiraterone with calf thymus DNA: Investigation with spectroscopic technique and modelling studies

Wani

Alsaif

Bakheit

et al. 2020

Bioorganic Chemistry

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Binding of toxic ligands to DNA could result in undesirable biological processes, such as carcinogenesis or mutagenesis. Binding mode of Abiraterone (ABR), a steroid drug and ctDNA(calf thymus DNA was investigated in this study using fluorescence and ultraviolet-visible spectroscopy. The probable prediction of binding and the type of interaction forces involved in the arrangement between ABR and ctDNA were explored through spectroscopic and molecular docking studies. The results indicated the binding of ABR to ctDNA in the minor groove. The binding constants were in the range of 1.35 × 10 6 -0.36× 10 6 L mol -1 at the studied temperatures.Fluorescence and spectrophotometric data suggested static quenching between ctDNA and ABR The endothermic values of thermodynamic parameters ΔH = -82.8 kJ mol −1 ; ΔS = -161 J mol −1 K −1 suggested that hydrogen bonding is the main force involved in binding ctDNA and ABR. In experimental studies the free binding energy at 298K was −34.9 kJ mol −1 with the relative binding energy ≈ −29.65 kJ mol −1 of docked structure. The Ksv obtained for ABR-KI was similar to that for ABR-ctDNA -KI demonstrating no protection by ctDNA against quenching effect of KI. Thus, suggesting involvement of groove binding between ABR and ctDNA. No change in the fluorescence intensity of ABR-ctDNA was observed in presence of NaCl. Thus, ruling out the involvement of electrostatic interaction. These studies could serve as new insights in understanding the mechanisms of toxicity, resistance and side effects of ABR.

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Study on the interaction of antidiabetic drug Pioglitazone with calf thymus DNA using spectroscopic techniques

Cited by 18 publications

References 51 publications

Interaction of aloe active compounds with calf thymus DNA

Interaction of aloe active compounds with calf thymus DNA

Competitive Binding of Novel Cyanine Dye AK3-5 and Europium Coordination Complexes to DNA

Interaction of an abiraterone with calf thymus DNA: Investigation with spectroscopic technique and modelling studies

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