Study on the Interaction of Vitamin B<sub>12</sub> with DNA by Spectroscopy and Electrochemical Methods

Hajian, Reza; Zafari, Mitra

doi:10.1002/cjoc.201180254

Cited by 10 publications

(4 citation statements)

References 30 publications

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“…Where, A and A 0 are the absorbance intensity of 5‐DDMP complex with dsDNA and 5‐DDMP, respectively. K is the binding constant, ϵ G and ϵ H–G are the absorptivity coefficients of 5‐DDMP and 5‐DDMP‐dsDNA complex, respectively and [DNA] is the molar concentration of dsDNA [36, 37]. The binding constant value came out to be 2.15×10 3 L mol −1 calculated by dividing intercept with slope of plot of A 0 /( A − A 0 ) vs 1/[DNA].…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Biosensor Design with Multi‐walled Carbon Nanotube to Display DNA‐Schiff Base Interaction

et al. 2021

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This paper demonstrates a Schiff base i. e. 5‐(diethylamino)‐2‐((2,6‐diethylphenylimino)methyl)phenol (5‐DDMP) that was sensed by DNA biosensor. dsDNA was immobilized onto GCE modified with functionalized multi‐walled carbon nanotubes to prepare a biosensor. The efficiency of dsDNA biosensor was determined and binding of 5‐DDMP with dsDNA was searched by UV‐vis spectrophotometry and differential pulse voltammetry. Molecular docking simulations between 5‐DDMP and dsDNA were explored and as a result, a hydrogen bond and a π‐π contact were observed between 5‐DDMP and deoxyguanosine base (dG22) of the strand B, deoxyadenosine base (dA5) of the strand A, respectively. These studies could be useful for new anticancer drug design and development.

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Section: Resultsmentioning

confidence: 99%

Electrochemical Biosensor Design with Multi‐walled Carbon Nanotube to Display DNA‐Schiff Base Interaction

et al. 2021

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“…A simplified mechanism based on the literature reports for the oxidation reactions catalyzed by metal complexes is depicted in Scheme 6. [31,32] Formation of oxo-metal species produced in the presence of hydrogen peroxide is the critical step in the process of alcohol oxidation.…”

Section: Oxidation Of Benzyl Alcohol and 4-methoxy Benzyl Alcohol In mentioning

confidence: 99%

Polymeric Schiff Base Metal Complexes Based on Thermo‐Responsive PNIPAM: Synthesis, Characterization and Catalytic Activity

Ghasemi

Andami

2017

ChemistrySelect

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The preparation and characterization of some polymeric Schiff base‐metal complexes based on thermo‐responsive copolymer composed of N‐isopropylacrylamide and acrylamide (poly (NIPAM‐co‐AAM)) is explained. Poly (NIPAM‐co‐AAM) was produced via conventional radical polymerization method with phase transition around 35 °C. The copolymer was amino modified followed by reaction with different aldehydes to produce polymeric Schiff‐bases and utilized as an absorbent for heavy metals. The polymeric complexes were used in the role of catalyst for hydrogen peroxide decomposition as a sample reaction. The significant characteristic of these thermo‐responsive copolymers is facilitating the catalyst recovery from the reaction components by heating above the lower critical solution temperature (LCST). The effect of different parameters such as ligand and metal ion type and reaction temperature was explored on the activity of these polymeric metal catalysts.

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“…In order to further investigate the interaction mode of CPT-11 with ds-DNA, the binding constant between drug-DNA at different temperatures and ionic strengths was calculated according to the doublereciprocal equation [23][24][25]:…”

Section: Ermodynamic Constantsmentioning

confidence: 99%

“…), the charge on CPT-11 molecule ( ) can then be calculated and the value of +0.47 is obtained. e SK value can also be used to calculate the polyelectrolyte standard free energy change (Δ 0 ) contribution to the overall free energy change (Δ ∘ ) at a given KCl concentration by the relation [13,[21][22][23][24][25][26][27][28][29][30][31][32][33][34]:…”

Section: Salt-dependent Binding Of Cpt-11mentioning

confidence: 99%

Spectrophotometric Studies on the Thermodynamics of the ds-DNA Interaction with Irinotecan for a Better Understanding of Anticancer Drug-DNA Interactions

Hajian

Tan

2013

Journal of Spectroscopy

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The ds-DNA binding properties of irinotecan (CPT-11) including binding constant, thermodynamic parameter, and thermal denaturation (Tm) have been systematically studied by spectrophotometric methods. The binding of CPT-11 to ds-DNA is quite strong as indicated by its remarkable hypochromicity and equilibrium binding constant (Kb). The van't Hoff plot of 1/Tversus ln Kbsuggests that the CPT-11 binds endothermically to ct-DNA which is characterized by large positive enthalpy and entropy changes. According to the polyelectrolyte theory, the charge release (Z), when ct-DNA interacts with CPT-11, is +0.98, which corresponds very well to the one positive charge carried by CPT-11. TheKbat a low concentration of salt is dominated by electrostatic interaction (98.5%) while that at a high concentration of salt is weakly controlled by nonelectrostatic processes (19.0%). A moderate stabilization of the double helix ds-DNA occurs when CPT-11 binds to ds-DNA as indicated by the increase inTmof ct-DNA by approximately 15°C in the presence of CPT-11. The CPT-11 is stabilized by intercalation in the DNA (binding constant,K[irinotecan-DNA] = 5.8 × 104 mol−1 L) and displaces the NR dye from the NR-DNA complex (K[NR-DNA] = 2.7 × 104 mol−1 L) in a competitive reaction.

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Study on the Interaction of Vitamin B₁₂ with DNA by Spectroscopy and Electrochemical Methods

Cited by 10 publications

References 30 publications

Electrochemical Biosensor Design with Multi‐walled Carbon Nanotube to Display DNA‐Schiff Base Interaction

Electrochemical Biosensor Design with Multi‐walled Carbon Nanotube to Display DNA‐Schiff Base Interaction

Polymeric Schiff Base Metal Complexes Based on Thermo‐Responsive PNIPAM: Synthesis, Characterization and Catalytic Activity

Spectrophotometric Studies on the Thermodynamics of the ds-DNA Interaction with Irinotecan for a Better Understanding of Anticancer Drug-DNA Interactions

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Study on the Interaction of Vitamin B12 with DNA by Spectroscopy and Electrochemical Methods

Cited by 10 publications

References 30 publications

Electrochemical Biosensor Design with Multi‐walled Carbon Nanotube to Display DNA‐Schiff Base Interaction

Electrochemical Biosensor Design with Multi‐walled Carbon Nanotube to Display DNA‐Schiff Base Interaction

Polymeric Schiff Base Metal Complexes Based on Thermo‐Responsive PNIPAM: Synthesis, Characterization and Catalytic Activity

Spectrophotometric Studies on the Thermodynamics of the ds-DNA Interaction with Irinotecan for a Better Understanding of Anticancer Drug-DNA Interactions

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Study on the Interaction of Vitamin B₁₂ with DNA by Spectroscopy and Electrochemical Methods