Study on the interaction between three benzimidazole anthelmintics and eosin Y by high performance liquid chromatography associating with resonance light scattering and its application

Abstract

A novel, highly selective, and sensitive resonance light scattering (RLS) detection approach coupled with high performance liquid chromatography (HPLC) was researched and developed for the synchronous analysis of three kinds of benzimidazole anthelmintics, including mebendazole (MBZ), albendazole (ABZ), and fenbendazole (FBZ) for the first time. In the pH range of 3.5-3.7 Britton-Robinson buffer medium, three kinds of anthelmintics, which were separated by HPLC, reacted with eosin Y (EY) to form 1:1 ion-association complexes, resulting in significantly enhanced RLS signals and the maximum peak located at 335 nm. The enhanced RLS intensity was in proportion to the MBZ, ABZ, and FBZ concentration in the range 0.2-25, 0.2-23, and 0.15-20 μg/mL, respectively. The limit of detection was in the range of 0.064-0.16 μg/mL. In addition, human urine was determined to validate the proposed method by spiked samples and real urine samples. Satisfactory results were obtained by HPLC-RLS method. Graphical Abstract The diagram mechanism of generating resonance between emitted light and scattered light.

High‐performance liquid chromatography study of gatifloxacin and sparfloxacin using erythrosine as post‐column resonance Rayleigh scattering reagent and mechanism study

“…As a new analytical technique, the RRS detection approach has attracted more and more attention from researchers. When an excited electron returns to the ground state and releases energy in the form of light, the electron absorbs an electromagnetic wave frequency that is equal to its scattering frequency, therefore, compared with single Rayleigh scattering (RS), RRS is stronger because re‐scattering takes places . Therefore in the past few years, RRS analysis technology has been widely applied in many fields, for instance, inorganic substance, pharmaceuticals, nucleic acid, natural base, etc.…”

Utilization of Eosin Dye as an Ion Pairing Agent for Determination of Pharmaceuticals: A Brief Review

“…Catalyst-free activation of peroxides through photoexcited electron under visible LED light irradiation was also performed using eosin Y as a model dye [25]. A highly selective and sensitive resonance light scattering detection approach was developed for the synchronous analysis of anthelmintics after reaction with eosin Y to form ionassociation complexes and separated by high-performance liquid chromatography [26]. An environmentally useful method was developed to detect sodium dodecyl sulphate using eosin Y and polyethyleneimine complex [27].…”