Study on the in vitro development of Chrysanthymum indicum L. shoots in the salinity stress

Abstract

In this study, NaCl at varrious concentrations of 4 – 10 g/L was used to investigate the salt tolerance of in vitro shoot cuttings of Chrysanthemum indicum. Morphological, physiological and biochemical changes during the response of shoot cuttings in the salinity stress were analyzed. NaCl at 6 g/L reduced the development of shoot cuttings. Under salinity stress conditions, there have just a little reduction of the chloroplast in parenchymal cells near the midrib of leaf before they turn brown and die. Besides, carotenoid, starch content, and photosynthesis intensity were decreased. In contrast, respiration rate, proline and total soluble sugar content, and the activity of IAA and gibberellin were strongly increased. The application of IAA 0.25 mg/L, zeatin 0.1 mg/L and GA3 0.1 mg/L improved the shoot development in the salinity stress condition. Shoots in MS medium supplemented with BA 0.2 mg/L, NAA 2 mg/L and NaCl 6 g/L grow better in salinity stress condition.

Effects of sodium silicate on the growth and starch content in seeds of Mungbean (Vigna radiate L.) under salt stress

“…As data in table 4, the 125 mM NaCl treatment has decreased chlorophyll a content, but NaCl increased carotenoid content compared to the control. Precedent studies showed that the impact of salt stress decreased chlorophyll content in Chrysanthemum indicum, Hordeum vulgare, Glycine max [22][23][24]. The suppression of chlorophyll synthesis and the activation of chlorophyll breakdown by the enzyme chlorophyllase have been ascribed to the reduction in chlorophyll levels in salt-stressed plants [25].…”