Study on the immobilization of carbonic anhydrases on geopolymer microspheres for CO2 capture

Chang, Shan; He, Yan; Yin-xi, LI; Cui, Xuemin

doi:10.1016/j.jclepro.2021.128163

Cited by 21 publications

(10 citation statements)

References 36 publications

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“…Furthermore, it was found that the V max values of free CA and CANF were, respectively, 166.66 μmol mL −1 min −1 and 185.18 μmol mL −1 min −1 (Table 1). A significant increase in K m value (from 7.6 mM to 21.5 mM) and V max value (from 1.6 mM min −1 to 5.0 mM min −1 ) was observed for CA immobilized on geopolymer microsphere 51 . In the previous study, the V max value of β‐glucosidase immobilized in alginate beads decreased from 0.94 μmol min −1 mL −1 to 0.74 μmol min −1 mL −1 48 .…”

Section: Resultsmentioning

confidence: 92%

“…A significant increase in K m value (from 7.6 mM to 21.5 mM) and V max value (from 1.6 mM min −1 to 5.0 mM min −1 ) was observed for CA immobilized on geopolymer microsphere. 51 In the previous study, the V max value of ⊎-glucosidase immobilized in alginate beads decreased from 0.94 μmol min −1 mL −1 www.soci.org T Sharma, AK Nadda wileyonlinelibrary.com/jctb to 0.74 μmol min −1 mL −1 . 48 The decrease in V max after immobilization is most likely due to the interaction of the biocatalyst with functional moieties present on the surface of the matrices.…”

Section: Kinetic Analysismentioning

confidence: 91%

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Protein inorganic hybrid nanoflowers of a microbial carbonic anhydrase as efficient tool for the conversion of CO₂ into value added product

Sharma

Nadda

2023

J of Chemical Tech & Biotech

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BACKGROUND: Carbonic anhydrase (CA) is one of the most widely distributed enzymes among plants, animals, and microorganisms, and it has an enormous ability to capture carbon dioxide (CO 2 ). However, the real-time application of CA is still a challenge due to its low operational stability, its difficulty in recovery from the reaction medium, and its poor durability.RESULTS: The synthesis of insoluble protein inorganic hybrid structures at nanoscale was proven as quite useful to catalyze enzymatic biotransformation. Here, CA nanoflowers (CANF) were synthesized with the self-assembly of metal phosphate and CA. The synthesis of CANF was performed using 0.2 mg mL −1 protein and 2.0 mM CuSO 4 at 4 °C under mild shaking conditions. The CANF exhibited optimum activity at pH 7.5 and a temperature of 40°C. The synthesized CANF were used for CO 2 conversion under optimized conditions and their kinetic parameters were studied using p-NPA hydrolysis. The V max and K m of CANF were 185.18 ∼mol min −1 mL −1 and 4.72 mM, compared with those of free CA, 166.66 ∼mol min −1 mL −1 and 5.12 mM, respectively. The stability of CANF has improved remarkably. The CANF made of metal ions and protein showed higher stability and enzyme activity than free enzymes. Furthermore, the CANF showed good reusability due to their mechanical properties and monodispersity. The production of CaCO 3 by CANF was 1.71-fold higher than that by free CA. CONCLUSION:The newly formed CANF showed flower-like morphology with good catalytic activity. This study demonstrated that CANF technology has a bright future in the conversion of CO 2 into CaCO 3 .

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Section: Resultsmentioning

confidence: 92%

Section: Kinetic Analysismentioning

confidence: 91%

Protein inorganic hybrid nanoflowers of a microbial carbonic anhydrase as efficient tool for the conversion of CO₂ into value added product

Sharma

Nadda

2023

J of Chemical Tech & Biotech

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“…After processing, the geopolymer could directly be treated as structural material, but also as a material carrier for other substances to improve the performance of the original material, such as enhancing the compressive strength, bending strength, acid and alkali resistance of the original material. Immobilization of carbonic anhydrase on geopolymer microspheres significantly improves the performance of carbonic anhydrase and enhances carbon dioxide capture and sequestration by immobilizing the enzyme [ 67 ]. In addition, geopolymer-biomass mesoporous carbon composites with mesoporous and macroporous structures are used for solar energy harvesting and could efficiently generate water vapor when used in conjunction with corresponding devices [ 68 ].…”

Section: Geopolymer Reaction Mechanism and Research Statusmentioning

confidence: 99%

Research and Application Progress of Geopolymers in Adsorption: A Review

Zhao

et al. 2022

Nanomaterials

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Geopolymer is a porous inorganic material with a three-dimensional mesh structure, good mechanical properties, a simple preparation process (no sintering) and a low economic cost, and it is environmentally friendly. Geopolymer concrete has been widely used in the construction field, and many other studies have revealed that geopolymer will become one of the most promising inorganic materials with unique structure and properties. This paper provides a review of the development and current status of geopolymers and briefly explains the effects of material proportioning, experimental factors and activators on geopolymer performance. Because of the advantages of high specific surface area and high porosity, geopolymers could be used as adsorbent materials. This paper summarizes the research progresses of the adsorption of metal cations, anions, dyes, and gases by geopolymers, which emphasizes the geopolymer membranes in adsorption, and discusses the challenges and opportunities for the development of more efficient, sustainable and practical adsorption protocols.

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“…Overall, immobilization technology enhances the thermal stability of enzymes and their resistance to adverse environments [ 105 ] while the reaction conditions are milder [ 106 ], compensating for the disadvantages of free enzymes, such as poor storage, poor operational stability, and difficult recovery [ 107 ]. However, immobilized enzymes still have limitations; for example, immobilized carriers can affect mass transfer efficiency, and enzyme activity can be affected by the carrier, which affects the removal of pollutants.…”

Section: Overview Of Immobilization Technologymentioning

confidence: 99%

Immobilization of Biomass Materials for Removal of Refractory Organic Pollutants from Wastewater

Liu

Yang

Zhang

et al. 2022

IJERPH

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In the field of environmental science and engineering, microorganisms, enzymes and algae are promising biomass materials that can effectively degrade pollutants. However, problems such as poor environmental adaptability, recycling difficulties, and secondary pollution exist in the practical application of non-immobilized biomass materials. Biomass immobilization is a novel environmental remediation technology that can effectively solve these problems. Compared with non-immobilized biomass, immobilized biomass materials have the advantages of reusability and stability in terms of pH, temperature, handling, and storage. Many researchers have studied immobilization technology (i.e., methods, carriers, and biomass types) and its applications for removing refractory organic pollutants. Based on this, this paper reviews biomass immobilization technology, outlines the mechanisms and factors affecting the removal of refractory organic pollutants, and introduces the application of immobilized biomass materials as fillers for reactors in water purification. This review provides some practical references for the preparation and application of immobilized biomass materials and promotes further research and development to expand the application range of this material for water purification.

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Study on the immobilization of carbonic anhydrases on geopolymer microspheres for CO2 capture

Cited by 21 publications

References 36 publications

Protein inorganic hybrid nanoflowers of a microbial carbonic anhydrase as efficient tool for the conversion of CO₂ into value added product

Protein inorganic hybrid nanoflowers of a microbial carbonic anhydrase as efficient tool for the conversion of CO₂ into value added product

Research and Application Progress of Geopolymers in Adsorption: A Review

Immobilization of Biomass Materials for Removal of Refractory Organic Pollutants from Wastewater

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Study on the immobilization of carbonic anhydrases on geopolymer microspheres for CO2 capture

Cited by 21 publications

References 36 publications

Protein inorganic hybrid nanoflowers of a microbial carbonic anhydrase as efficient tool for the conversion of CO2 into value added product

Protein inorganic hybrid nanoflowers of a microbial carbonic anhydrase as efficient tool for the conversion of CO2 into value added product

Research and Application Progress of Geopolymers in Adsorption: A Review

Immobilization of Biomass Materials for Removal of Refractory Organic Pollutants from Wastewater

Contact Info

Product

Resources

About

Protein inorganic hybrid nanoflowers of a microbial carbonic anhydrase as efficient tool for the conversion of CO₂ into value added product

Protein inorganic hybrid nanoflowers of a microbial carbonic anhydrase as efficient tool for the conversion of CO₂ into value added product