Study on the Factors Influencing the Extraction of Chenodeoxycholic Acid from Duck Bile Paste by Calcium Salt Method

Abstract

New extraction technology of chenodeoxycholic acid from duck bile paste by calcium salt was investigated. The optimum conditions of extraction were determined by orthogonal experimental design. The results indicated that influencing factors on the extraction efficiency of chenodeoxycholic acid were as follows: hydrogen peroxide, methyl alcohol, glacial acetic acid, and calcium chloride. The optimum extracting conditions of chenodeoxycholic acid were 1000 mL amount of methyl alcohol, 50 mL amount of hydrogen peroxide, 500 mL amount of 20% calcium chloride, and 600 mL amount of 60% glacial acetic acid for a quantity of duck paste. The yield of chenodeoxycholic acid was 30%.

The diversity of ursodeoxycholic acid precursors from bile waste of commercially available fishes, poultry and livestock in Indonesia

“…It contains high quantities of CA and DCA but trace amounts of CDCA and LA, which are also confirmed in this experiment. However, CA of that bovine bile is mostly present in a conjugated form (Watanabe, Tsuneyama, 2012;Hu, Feng, Zhang, 2018) and therefore need to be deconjugated to enhance the yield of free CA. This process uses a large quantity of alkaline, high temperature, and for extremely long times (Tonin, Arends, 2018) which eventually makes the process consumes higher cost and not environmentally friendly.…”

Effects of formulation and processing techniques on physicochemical properties of surimi gel

“…초기 담즙산 분석은 높은 감도를 가진 gas chromatography mass spectrometry (GC-MS)를 이용한 분석이 많이 진행되었지만 (Grundy et al, 1965;Koopman et al, 1984;Street et al, 1986;Batta et al, 1992;Batta et al, 1997, Batta andsalen, 1999;Tsai et al, 2011) (Chang et al, 2003;Jones et al, 2003;Mukharjee and Pal, 2011 (Tessier et al, 2003;Burkard et al, 2005;Alnouti et al, 2008;Huang et al, 2011;John et al, 2014) (Wan et al, 2012;Hu et al, 2018).…”