Study on the experimental ulcerative colitis(UC) model induced by dextran sulfate sodium(DSS) in rats. (2).

伊佐美, 木村; 忍, 永濱; 真規, 川崎; 明美, 神谷; 美紀子, 片岡

doi:10.1254/fpj.105.145

Cited by 12 publications

(3 citation statements)

References 5 publications

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“…Numerous reports have been published concerning experiments using animal models of ulcerative colitis, i.e. rodent with DSS-induced colitis [7][8][9] . It is thought that NF-κB is associated with the expression of various cytokines, chemokines, and adhesion factors in inflammation.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the effect of pyrrolidine dithiocarbamate in suppressing inflammation in mice with dextran sodium sulfate-induced colitis

Hirata¹

2007

WJG

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AIM:To evaluate the effect of pyrrolidine dithiocarbamate (PDTC; an NF-κB inhibitor) administered at low (50 mg/kg) and high (100 mg/kg) doses in suppressing colitis in mice with dextran sodium sulfate (DSS)-induced colitis. METHODS:Mice were divided into a DSS-untreated group (normal group), DSS-treated control group, DSS+PDTC-treated groupⅠ(low-dose group), and DSS+PDTC-treated groupⅡ (high-dose group). In each group, the disease activity index score (DAI score), intestinal length, histological score, and the levels of activated NF-κB and inflammatory cytokines (IL-1β and TNF-α) in tissue were measured. RESULTS:The DSS+PDTC-treated groupⅡ exhibited suppression of shortening of intestinal length and reduction of DAI score. Activated NF-κB level and IL-1β and TNF-α levels were significantly lower in DSS+PDTC-treated groupⅡ. CONCLUSION:These findings suggest that PDTC is useful for the treatment of ulcerative colitis.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the effect of pyrrolidine dithiocarbamate in suppressing inflammation in mice with dextran sodium sulfate-induced colitis

Hirata¹

2007

WJG

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“…This model has similarity with human UC, including symptoms such as loose stool, hematochezia, body weight loss, and histopathology (1,3,4). In this study, we employed a disease-sustained colitis model by drinking 1% DSS after the appearance of obvious disease induced by 3% DSS (6). This model enabled us to evaluate the therapeutic efficacy of rebamipide enema (7).…”

Section: Discussionmentioning

confidence: 99%

“…All rats had an access a drinking bottle containing water for 3 days. Subsequently, DSS (MW 5000, Wako Pure Chemical Industry Ltd., Osaka, Japan) was dissolved in de-ionized pure water (milli-Q SP Reagent Water System, Millipore, Billerica, Massachusetts) at a concentration of 3%, and given to rats as drinking water from the first experimental day (day 0) to induce colitis, as previously described (6). Rats drinking with de-ionized pure water without DSS or drug treatments were designated as the normal control group.…”

Section: Animal Experimentsmentioning

confidence: 99%

Rebamipide Enema is Effective for Treatment of Experimental Dextran Sulfate Sodium Induced Colitis in Rats

et al. 2005

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We investigated therapeutic efficacy of rebamipide using dextran sulfate sodium (DSS) induced colitis model in rats. Three percent DSS solution was given to rats for 9 days. After that, we evaluated the drug efficacy on colitis sustained with continuous drinking of 1% DSS. Twice-daily treatment with 0.3% or 1% rebamipide for 14 days significantly ameliorated the stool abnormality in the colitis model, preferentially suppressed hematochezia. The colonic mucosal lesion, determined by Alcian blue staining on day 24, was significantly reduced by rebamipide enema in a dose-dependent manner. Either rebamipide or 5-aminosalycilic acid (5-ASA) enema treated once daily significantly ameliorated colitis. The minimum effective dose of rebamipide was 0.3% in once-daily treatment, and that of 5-ASA was 10%. In a mechanistic study, the epithelial cell sheet formation of the T84 colon cancer cell was measured as an increase in generation of trans-epithelial electrical resistance in vitro. Rebamipide accelerated the increase, while 5-ASA conversely suppressed it. These results suggest that rebamipide enema is effective for treatment of experimental ulcerative colitis (UC).

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Dextran sulfate sodium (DSS) induced experimental colitis in immunodeficient mice: Effects in CD4+-cell depleted, athymic and NK-cell depleted SCID mice

Landström²,

et al. 1996

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Administration of dextran sulfate to mice, given in the drinking water results in acute or subacute colonic inflammation, depending on the administration protocol. This colonic inflammation exhibits ulceration, healing and repair, and a therapeutic response that makes it valuable for the study of mechanisms that could act in the pathogenesis of human ulcerative colitis, a disease thought to have an immunologically dependent pathogenesis. To investigate if immunological mechanisms were involved in the induction of colonic inflammation in this model, mice with different degrees of immunodeficiency were used. It was shown that dextran sulfate induced colitis could be induced in Balb/c mice depleted of CD4(+) helper T cells by treatment with monoclonal antibodies preceded by adult thymectomy. The depletion of CD4(+) was verified by flow cytometric analysis. Furthermore, the colonic inflammation could equally be induced in athymic CD-1 nu/nu mice lacking thymus-derived T cells, in T and B-cell deficient SCID mice, and also in SCID mice depleted of NK cells by treatment with anti-asialo GM1 antibodies. The NK-cell depletion was verified by measuring spleen NK-cell activity. The resulting colonic inflammation in all these types of deficient mice was qualitatively comparable, as shown by clinical and histological appearance. These results indicate that the presence of functional T, B and NK cells is not crucial for the induction of dextran sulfate colitis in mice.

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Study on the experimental ulcerative colitis(UC) model induced by dextran sulfate sodium(DSS) in rats. (2).

Cited by 12 publications

References 5 publications

Evaluation of the effect of pyrrolidine dithiocarbamate in suppressing inflammation in mice with dextran sodium sulfate-induced colitis

Evaluation of the effect of pyrrolidine dithiocarbamate in suppressing inflammation in mice with dextran sodium sulfate-induced colitis

Rebamipide Enema is Effective for Treatment of Experimental Dextran Sulfate Sodium Induced Colitis in Rats

Dextran sulfate sodium (DSS) induced experimental colitis in immunodeficient mice: Effects in CD4+-cell depleted, athymic and NK-cell depleted SCID mice

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