Study on the electrochemical detection of the macrolide antibiotics clarithromycin and roxithromycin in reversed-phase high-performance liquid chromatography

Pappa-Louisi, A.; Papageorgiou, A.; Zitrou, A; Sotiropoulos, S.; Georgarakis, E.; Zougrou, F.

doi:10.1016/s0378-4347(00)00614-9

Cited by 51 publications

(30 citation statements)

References 14 publications

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“…Due to weak UV absorbance of macrolide antibiotics, it is difficult to develop a simple method for analysis of the drugs in biological fluids using conventional UV detection, thus, high-performance liquid chromatography (HPLC) with electrochemical detection (ED) is the standard method for analysis of the drugs in biological matrix. Several HPLC methods have been reported for analysis of clarithromycin in human serum using EC [2][3][4][5][6][7][8][9], MS [10], UV [11] and fluorescence detections [12]. Determination of the drug in serum samples using HPLC with UV detection at 205 nm has been recently reported by Amini and Ahmadiani [11] and although, limit of quantification (LOQ) of 31.25 ng mL −1 has been obtained using injection volume of 100 L, however, due to weak UV absorbance of the drug the system should be set at highest sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Determination of clarithromycin in human serum by high-performance liquid chromatography after pre-column derivatization with 9-fluorenylmethyl chloroformate: Application to a bioequivalence study

Bahrami

Mohammadi

2007

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

Determination of clarithromycin in human serum by high-performance liquid chromatography after pre-column derivatization with 9-fluorenylmethyl chloroformate: Application to a bioequivalence study

Bahrami

Mohammadi

2007

Journal of Chromatography B

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“…3 Numerous analytical methods have been reported to quantify RXT in bulk, dosage forms, and biological fluids, and the methods were usually based on high performance liquid chromatography (HPLC) coupled with ultraviolet (UV), [4][5][6] fluorescence, 7 electrochemical detection, [8][9][10] or mass spectrometry. 11,12 RXT is listed in a number of official compendia including European Pharmacopoeia (EP), Japanese Pharmacopoeia (JP), and Korean Pharmacopoeia (KP), and all the compendial methods for RXT employ HPLC-UVbased analytical methods.…”

Section: Compared To Erythromycin Plasma Concentrationsmentioning

confidence: 99%

Optimization of the experimental conditions for determination of roxithromycin in bulk and dosage forms

Jeong¹,

Lee²,

Kim³

et al. 2017

Analytical Science and Technology

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Roxithromycin (RXT), which is an antibiotic used to treat respiratory tract and urinary infections, is official in Korean Pharmacopoeia (KP) and is marketed in various dosage forms including tablet, granule, suspension, and tablet for suspension in Korea. This study presents how a universal and reliable method to quantify RXT in bulk drug and formulations was developed. Effects of factors including column type, buffer concentration, type and concentration of organic solvent, buffer pH, and type and concentration of mobile phase additive, were examined, and some categorical or crucial factors including the types of column, organic solvent, mobile phase additive and the buffer pH were optimized by one-factor-at-a-time approach. Subsequently, concentrations of the buffer and additive and column temperature were optimized by response surface methodology using Box-Behnken design aiming to acquire the RXT peak of good shape. The optimized method employed a Phenomenex Gemini 5μ C18 110A (150 × 4.60 mm, 5 μm) maintained at 30°C with the mobile phase consisting of 25 mM phosphate buffer (pH 6.0) with 0.3 % tetrabutylammonium hydroxide and methanol at a ratio of 37:63 (v/v). Method validation results showed that the developed method was linear, precise, and accurate. Compared to the compendial methods in KP 10 that exhibited a significant tailing of the RXT peak despite using unfavorably high buffer concentrations and were not harmonized among bulk drug and formulations, this method could be universally applied to RXT bulk drug and marketed products in various dosage forms and thus was adopted in KP 11.

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“…It is compatible with a much wider range of solvents and modifiers and produces a stable baseline even in gradient elution [1][2][3][4][5][6] . Macrolide antibiotics have been used for the treatment of bacterial infections caused by Grampositive organismus 7 . Clarithromycin is a relatively new semisynthetic macrolide antibiotic derived from erythromycin A and consists of a 14-membered lactone ring with sugars linked via glycosidic bonds.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of an HPLC Method for the Determination of the macrolide antibiotic Clarithromycin using Evaporative Light Scattering Detector in raw materials and Pharmaceutical Formulations

Tzouganaki

Koupparis

2017

Mediterr.J.Chem.,

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In this work, ELS-Detector has been used for the development of an HPLC method for the determination of clarithromycin in pharmaceutical formulations (tablets and pediatric suspension). Isocratic reversed phase HPLC approach has been developed using a C-18 column (Waters Spherisorb 5 μm ODS2, 4.6x250 mm) and a mobile phase consisting of acetonitrile / aqueous trifluoroacetic acid as pairing reagent. Experimental parameters (temperature of heated drift tube, flow rate of mobile phase, gas flow rate, mobile phase composition) were optimized. Clarithromycin’ s stability was thoroughly examined in different solvent systems. Using the optimized conditions the working range was 5-100 μg/mL (upper limit can be increased considerably), with a detection limit of 4.5 μg/mL (6x10-6 M). The method was validated as per ICH guidelines. The retention time was 4.7 min. The method was successfully applied for the content assay of clarithromycin formulations.

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Study on the electrochemical detection of the macrolide antibiotics clarithromycin and roxithromycin in reversed-phase high-performance liquid chromatography

Cited by 51 publications

References 14 publications

Determination of clarithromycin in human serum by high-performance liquid chromatography after pre-column derivatization with 9-fluorenylmethyl chloroformate: Application to a bioequivalence study

Determination of clarithromycin in human serum by high-performance liquid chromatography after pre-column derivatization with 9-fluorenylmethyl chloroformate: Application to a bioequivalence study

Optimization of the experimental conditions for determination of roxithromycin in bulk and dosage forms

Development and Validation of an HPLC Method for the Determination of the macrolide antibiotic Clarithromycin using Evaporative Light Scattering Detector in raw materials and Pharmaceutical Formulations

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