Study on the effect of stress treatment on embryogenic callus of raspberry

Abstract

In order to obtain the embryogenic callus of raspberry, the friable calluses of raspberry were used as materials to be treated by different time of starvation and desiccation, and were further cultivated and induced embryoid. By use of embryogenic observation, the embryogenic degree of callus could be determined. The results showed that the embryogenic degree of calluses which were treated by starvation for 6 days was the highest, and the desiccation treatment had no effect on embryogenic degree of calluses. Therefore, appropriate starvation treatment can facilitate the transformation of non-embryogenic callus into embryogenic callus. The results of this experiment could provide reference for callus embryogenesis induction of other plants.