Study on the differences of metabolites and their bioactivities of Lithocarpus under different processing methods

Lou, Wenjing; Mu, Xuemei; Liu, J. X.; Xun, Mi; Hu, Yifeng

doi:10.1016/j.fbio.2023.102817

Cited by 4 publications

(3 citation statements)

References 36 publications

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“…1 The therapeutic properties of sweet tea are attributed to its functional compounds, especially phloridzin and trilobatin, which have garnered considerable scientific attention in scholarly explorations. 2–4 Phloridzin is associated with a spectrum of pharmacodynamic properties, encompassing antioxidative, 5,6 anti-inflammatory, 7,8 and hepatoprotective effects. 9 On the other hand, trilobatin shows anti-diabetic, 10,11 neuroprotective, 12 and anti-obesity effects.…”

Section: Introductionmentioning

confidence: 99%

“…14 Current quantitative methods rely on large amounts of hazardous organic solvents, long analysis times, and multiple standard solutions. 2–4 Consequently, these methods fail to meet the demands of contemporary industry and green chemistry, which require high efficiency, cost-effectiveness, and environmental friendliness. Hence, the creation of a quantitative method that is not only more effective but also less expensive and eco-friendlier is imperative.…”

Section: Introductionmentioning

confidence: 99%

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Rapid and green quantification of phloridzin and trilobatin in Lithocarpus litseifolius (Hance) Chun (sweet tea) using an online pressurized liquid extraction high-performance liquid chromatography at equal absorption wavelength method

Qian,

Lei,

Tang

et al. 2024

Anal. Methods

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rapid and green quantification of phloridzin and trilobatin in Lithocarpus litseifolius (Hance) Chun (sweet tea) using an online pressurized liquid extraction high-performance liquid chromatography at equal absorption wavelength method

Qian,

Lei,

Tang

et al. 2024

Anal. Methods

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“…As the contradiction between the human desire for sweet taste and the demand for a healthier lifestyle has increased, the market need for low-calorie sweeteners has become substantially higher [4]. Being an extremely low-calorie natural sweetener, TBL has a sweetness 300 times higher than that of sucrose [5,6] and is thus a highly promising sugar substitute.…”

Section: Introductionmentioning

confidence: 99%

Optimizing Trilobatin Production via Screening and Modification of Glycosyltransferases

Yang,

Cheng,

Bai

et al. 2024

Molecules

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Trilobatin (TBL) is a key sweet compound from the traditional Chinese sweet tea plant (Rubus suavissimus S. Lee). Because of its intense sweetness, superior taste profile, and minimal caloric value, it serves as an exemplary natural dihydrochalcone sweetener. It also has various health benefits, including anti-inflammatory and glucose-lowering effects. It is primarily produced through botanical extraction, which impedes its scalability and cost-effectiveness. In a novel biotechnological approach, phloretin is used as a precursor that is transformed into TBL by the glycosyltransferase enzyme ph-4′-OGT. However, this enzyme’s low catalytic efficiency and by-product formation limit the large-scale synthesis of TBL. In our study, the enzyme Mdph-4′-OGT was used to screen 17 sequences across species for TBL synthesis, of which seven exhibited catalytic activity. Notably, PT577 exhibited an unparalleled 97.3% conversion yield within 3 h. We then optimized the reaction conditions of PT577, attaining a peak TBL bioproduction of 163.3 mg/L. By employing virtual screening, we identified 25 mutation sites for PT577, thereby creating mutant strains that reduced by-products by up to 50%. This research enhances the enzymatic precision for TBL biosynthesis and offers a robust foundation for its industrial-scale production, with broader implications for the engineering and in silico analysis of glycosyltransferases.

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Chromosome-scale genome assembly of sweet tea (Lithocarpus polystachyus Rehder)

Liu,

Zhang,

Zhou

et al. 2023

Sci Data

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Lithocarpus, with >320 species, is the second largest genus of Fagaceae. However, the lack of a reference genome limits the molecular biology and functional study of Lithocarpus species. Here, we report the chromosome-scale genome assembly of sweet tea (Lithocarpus polystachyus Rehder), the first Lithocarpus species to be sequenced to date. Sweet tea has a 952-Mb genome, with a 21.4-Mb contig N50 value and 98.6% complete BUSCO score. In addition, the per-base consensus accuracy and completeness of the genome were estimated at 60.6 and 81.4, respectively. Genome annotation predicted 37,396 protein-coding genes, with repetitive sequences accounting for 64.2% of the genome. The genome did not undergo whole-genome duplication after the gamma (γ) hexaploidy event. Phylogenetic analysis showed that sweet tea diverged from the genus Quercus approximately at 59 million years ago. The high-quality genome assembly and gene annotation resources enrich the genomics of sweet tea, and will facilitate functional genomic studies in sweet tea and other Fagaceae species.

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Study on the differences of metabolites and their bioactivities of Lithocarpus under different processing methods

Cited by 4 publications

References 36 publications

Rapid and green quantification of phloridzin and trilobatin in Lithocarpus litseifolius (Hance) Chun (sweet tea) using an online pressurized liquid extraction high-performance liquid chromatography at equal absorption wavelength method

Rapid and green quantification of phloridzin and trilobatin in Lithocarpus litseifolius (Hance) Chun (sweet tea) using an online pressurized liquid extraction high-performance liquid chromatography at equal absorption wavelength method

Optimizing Trilobatin Production via Screening and Modification of Glycosyltransferases

Chromosome-scale genome assembly of sweet tea (Lithocarpus polystachyus Rehder)

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