Study on the biological effects of ZnO nanosheets on EBL cells

Li, Mei; Ma, Yushu; Lian, Xiaodi; Lü, Yan; Li, Yuanyuan; Yao, Xi; Sun, Xiaofeng

doi:10.3389/fbioe.2022.915749

Cited by 3 publications

(4 citation statements)

References 33 publications

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“…The structure observed was similar to that observed by Li et al. [41]. In undoped samples, stacking of the nanosheets was observed (Figures 4a and 4c), whereas in nitrogen‐doped samples, dispersed nanoflakes were found (Figures 4b and 4d).…”

Section: Resultssupporting

confidence: 87%

“…The average diameter of the sheets was determined using ImageJ software and is found to have a sheet length of about 368 nm and 47 nm for ZnO and 344 nm and 53 nm for NÀ ZnO (calculated from Figures 4a and 4b, respectively). The structure observed was similar to that observed by Li et al [41]. In undoped samples, stacking of the nanosheets was observed (Figures 4a and 4c), whereas in nitrogen-doped samples, dispersed nanoflakes were found (Figures 4b and 4d).…”

Section: Characterization Of As Prepared Zno and Nà Znosupporting

confidence: 87%

“…The lattice stripe spacing (d‐spacing) value is measured to be 0.281 nm, corresponding to the standard value of (101) direction (Figure 5c). The selected area diffraction pattern (SAED) pattern, shown in Figure 5d, confirms the polycrystalline nature of the samples and the crystal planes corresponding to the hexagonal wurtzite structure of zinc oxide [41]. The average length and thickness of the nanosheets were estimated from the distribution histograms from the TEM micrographs [42].…”

Section: Resultsmentioning

confidence: 60%

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Electrochemical pH sensing characteristics of nitrogen‐doped zinc oxide nanostructures using a screen‐printed platform**

Mary Manoj,

Rose Viannie

2023

Electroanalysis

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Monitoring pH variations is of vital importance in the field of medical diagnostics and healthcare devices. Employing zinc oxide (ZnO) nanomaterials as active sensing elements allows sensitivity enhancement by increasing the surface area of the nanomaterials used and improving the charge transfer mechanism in the sensor. In this study, an electrochemical pH sensor based on nitrogen‐doped zinc oxide nanosheets was developed using a single step hydrothermal technique. The results obtained show the successful incorporation of nitrogen into the crystal structure of ZnO nanosheets. The sensing platform was fabricated using a simple mask‐printing technique using carbon electrodes on a polyimide substrate. The sensing characteristics of nitrogen‐doped ZnO (N−ZnO) nanosheets as pH sensors are evaluated for the first time. The results show that the response time and performance improved with nitrogen doping, for a lower analyte volume of just 5 mL. Furthermore, the detailed mechanism of pH sensing was formulated using electrochemical impedance spectroscopy (EIS). The resistance obtained was directly proportional to the charge‐transfer resistance at the electrode‐electrolyte interface for N−ZnO. The sensitivity as determined by charge‐transfer resistance is 0.512 MΩ/pH. Further, the chronoamperometric studies show a sensitivity of 0.156 μA/pH. The response characteristics also reveal a linearity of 0.965 over a pH range of 3 to 9. Hence, the study shows the exceptional response of N−ZnO nanostructures in pH sensing applications. The advantages of the N−ZnO nanosheets include higher sensitivity, flexibility, and a smaller volume of testing fluid that promotes their easy integration into various analytical applications.

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Section: Resultssupporting

confidence: 87%

Section: Characterization Of As Prepared Zno and Nà Znosupporting

confidence: 87%

Section: Resultsmentioning

confidence: 60%

See 1 more Smart Citation

Electrochemical pH sensing characteristics of nitrogen‐doped zinc oxide nanostructures using a screen‐printed platform**

Mary Manoj,

Rose Viannie

2023

Electroanalysis

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“…Due to their water solubility, biocompatibility, low toxicity, and ease of modification, nanomaterials have been extensively researched for their potential to be combined with drugs. This combination aims to reduce side effects ( Li et al, 2020 ), improve drug efficacy ( Li et al, 2022 ), and enhance drug targeting ( Huang et al, 2019 ). For instance, a study reported the synthesis of highly biocompatible glycyrrhizic acid nanoparticles (GANPs) using glycyrrhizic acid (GA) as a raw material.…”

Section: Discussionmentioning

confidence: 99%

A dicoumarol-graphene oxide quantum dot polymer inhibits porcine reproductive and respiratory syndrome virus through the JAK-STAT signaling pathway

Li,

Wang,

Wang

et al. 2024

Front. Microbiol.

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IntroductionPorcine reproductive and respiratory syndrome virus (PRRSV) causes substantial economic losses in the global swine industry. The current vaccine options offer limited protection against PRRSV transmission, and there are no effective commercial antivirals available. Therefore, there is an urgent need to develop new antiviral strategies that slow global PRRSV transmission.MethodsIn this study, we synthesized a dicoumarol-graphene oxide quantum dot (DIC-GQD) polymer with excellent biocompatibility. This polymer was synthesized via an electrostatic adsorption method using the natural drug DIC and GQDs as raw materials.ResultsOur findings demonstrated that DIC exhibits high anti-PRRSV activity by inhibiting the PRRSV replication stage. The transcriptome sequencing analysis revealed that DIC treatment stimulates genes associated with the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signalling pathway. In porcine alveolar macrophages (PAMs), DIC-GQDs induce TYK2, JAK1, STAT1, and STAT2 phosphorylation, leading to the upregulation of JAK1, STAT1, STAT2, interferon-β (IFN-β) and interferon-stimulated genes (ISGs). Animal challenge experiments further confirmed that DIC-GQDs effectively alleviated clinical symptoms and pathological reactions in the lungs, spleen, and lymph nodes of PRRSV-infected pigs.DiscussionThese findings suggest that DIC-GQDs significantly inhibits PRRSV proliferation by activating the JAK/STAT signalling pathway. Therefore, DIC-GQDs hold promise as an alternative treatment for PRRSV infection.

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LppA is a novel plasminogen receptor of Mycoplasma bovis that contributes to adhesion by binding the host extracellular matrix and Annexin A2

Liu,

Li,

Lan

et al. 2023

Vet Res

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Mycoplasma bovis is responsible for various inflammatory diseases in cattle. The prevention and control of M. bovis are complicated by the absence of effective vaccines and the emergence of multidrug-resistant strains, resulting in substantial economic losses worldwide in the cattle industry. Lipoproteins, vital components of the Mycoplasmas cell membrane, are deemed potent antigens for eliciting immune responses in the host upon infection. However, the functions of lipoproteins in M. bovis remain underexplored due to their low sequence similarity with those of other bacteria and the scarcity of genetic manipulation tools for M. bovis. In this study, the lipoprotein LppA was identified in all examined M. bovis strains. Utilizing immunoelectron microscopy and Western blotting, it was observed that LppA localizes to the surface membrane. Recombinant LppA demonstrated dose-dependent adherence to the membrane of embryonic bovine lung (EBL) cells, and this adhesion was inhibited by anti-LppA serum. In vitro binding assays confirmed LppA’s ability to associate with fibronectin, collagen IV, laminin, vitronectin, plasminogen, and tPA, thereby facilitating the conversion of plasminogen to plasmin. Moreover, LppA was found to bind and enhance the accumulation of Annexin A2 (ANXA2) on the cell membrane. Disrupting LppA in M. bovis significantly diminished the bacterium’s capacity to adhere to EBL cells, underscoring LppA’s function as a bacterial adhesin. In conclusion, LppA emerges as a novel adhesion protein that interacts with multiple host extracellular matrix proteins and ANXA2, playing a crucial role in M. bovis’s adherence to host cells and dissemination. These insights substantially deepen our comprehension of the molecular pathogenesis of M. bovis.

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Study on the biological effects of ZnO nanosheets on EBL cells

Cited by 3 publications

References 33 publications

Electrochemical pH sensing characteristics of nitrogen‐doped zinc oxide nanostructures using a screen‐printed platform**

Electrochemical pH sensing characteristics of nitrogen‐doped zinc oxide nanostructures using a screen‐printed platform**

A dicoumarol-graphene oxide quantum dot polymer inhibits porcine reproductive and respiratory syndrome virus through the JAK-STAT signaling pathway

LppA is a novel plasminogen receptor of Mycoplasma bovis that contributes to adhesion by binding the host extracellular matrix and Annexin A2

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