Study on the aqueous two-phase systems composed of surfactant, ionic liquid and water

Wang, Xiuhong; Wei, Xilian; Liu, Jie; Liu, Jifeng; Sun, Dezhi; Du, Panpan; Ping, Ali

doi:10.1016/j.fluid.2013.03.004

Cited by 26 publications

(14 citation statements)

References 30 publications

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“…Therefore, it is important to determine experimentally the best system for each molecule, since size and aminoacid composition varies. In general, ATPS and ATPMS suit biological samples because each phase contains 60-90% water, providing a mild environment for active molecules [11,16,23], and in our results ATPMS presented the best system. In order to further characterize the application of this ATPMS, we tested enzymatic stability at two different temperatures (25.0 and 30.0°C), with different extraction times (2, 4, 6, 8, 12 and 16 h), different TX-114 concentrations (2.0%, 4.0%, 6.0%, 8.0%, 10.0% and 12.0% w/w) and different response variables were evaluated: %AB Lip , K Lip and %REC Bot .…”

Section: Discussionsupporting

confidence: 50%

“…In this case, systems are called aqueous two-phase micellar systems (ATPMS), and phase separation results from an increase in temperature, changes in pH, surfactant concentration and addition of salts [10]. Nonionic surfactants, such as octylphenol ethoxylate, Triton TM X-114 (TX-114) and different alkyl polyoxyethylene surfactants, display such temperaturesensitive phase separation, and can form ATPMS [16,23].…”

Section: Introductionmentioning

confidence: 99%

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Liquid–liquid extraction of lipase produced by psychrotrophic yeast Leucosporidium scottii L117 using aqueous two-phase systems

Duarte

Lopes

Molino

et al. 2015

Separation and Purification Technology

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a b s t r a c tAqueous two-phase systems (ATPS) have been used in biomolecules separation and as an efficient alternative to traditional purification systems for lipases extraction. Here, we investigated the partitioning and recovery of lipase derived from Leucosporidium scottii L117 using ATPS and aqueous two-phase micellar systems (ATPMS). Thus, we evaluated three ATPS: (i) polyethylene glycol (PEG)/phosphate salts and (ii) PEG/polyacrylic acid (NaPA) in different molecular weights (1500, 4000 and 8000 g/mol). (iii) Triton X-114 (TX-114)/McIlvaine buffer pH 7.0 in different conditions (2.0% (w/w) of TX-114 at 25.0 and 28.0°C). The PEG/phosphate and PEG/NaPA systems resulted in a great loss of enzymatic activity; thus these systems do not represent viable alternatives for these lipase extraction. The micellar systems yielded the best results for lipase extraction with enzyme activity balances ranging between 84.7% and 113.05%. After optimizing the micellar system by experimental design of the partition coefficient of lipase increased by 10.3-fold (0.75-7.76). Lipase preferentially partitioned into the micelle-rich phase with K Lip = 7.76, %REC Bot = 93.85% and PF = 1.2 at 25.03°C, 5.1 pH and 10.38% TX-114 and K Lip = 4.77, % REC Bot = 73.53% and PF = 1.97 at 28.00°C, 4.5 pH and 8.0% TX-114, indicating that the ATPMS represents an alternative to purification/extraction of lipase L. scottii L117. A crude lipase extract was also evaluated to define the optimum pH and temperature. Lipase reached optimal activity at 40°C, and remained stable in pH values ranging from pH 3.0 to 8.0 and temperatures from 20.0 to 45.0°C, with relative residual lipase activity above 80% after 30 min of incubation.

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Section: Discussionsupporting

confidence: 50%

Section: Introductionmentioning

confidence: 99%

Liquid–liquid extraction of lipase produced by psychrotrophic yeast Leucosporidium scottii L117 using aqueous two-phase systems

Duarte

Lopes

Molino

et al. 2015

Separation and Purification Technology

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“…ATPSs formed by two polymers or a polymer and a salt represent the conventional and most studied systems. However, other alternative biphasic systems have been proposed, such as polymer-surfactant [7], ionic liquid-salt [8] or surfactant-ionic liquid [9]. The different types of ATPSs available offer different features that can be explored to selectively separate biomolecules.…”

Section: Introductionmentioning

confidence: 99%

Polyethylene glycol 8000+ citrate salts aqueous two-phase systems: Relative hydrophobicity of the equilibrium phases

Silvério

Gracia

Teixeira

et al. 2016

Fluid Phase Equilibria

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Polyethylene glycol 8000 + citrate salts aqueous two-phase systems: relative hydrophobicity of the equilibrium phases, Fluid Phase Equilibria (2015), http://dx. AbstractThe Gibbs free energy of transfer of a methylene group, ∆G*(CH 2 ), is reported as a measure of the relative hydrophobicity of the equilibrium phases. Furthermore, ∆G*(CH 2 ) is a characteristic parameter of each tie-line, and for that reason can be used for comparing different tie-lines of a given aqueous two-phase system (ATPS) or even to establish comparisons among different ATPSs. In this work, the partition coefficients of a series of four dinitrophenylated-amino acids were experimentally determined, at 23 ºC, in five different tie-lines of PEG8000-(sodium or potassium) citrate ATPSs. ∆G*(CH 2 ) values were calculated from the partition coefficients and used to evaluate the relative hydrophobicity of the equilibrium phases. PEG8000-potassium citrate ATPSs presented larger relative hydrophobicity than PEG8000-sodium citrate ATPSs. Furthermore, the results obtained indicated that the PEG-rich phase (top phase) has higher affinity to participate in hydrophobic hydration interactions than the salt-rich phase (bottom phase).

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“…Molecular self-assembly is a kind of entity with a special structure, which associates spontaneously by molecules [ 1 , 2 , 3 , 4 , 5 , 6 ]. It not only promotes the development of functional and complex materials, but also leads to the formation of different aggregate morphologies such as crystalline structures, wormlike micelles, vesicles, and spherical micelles [ 7 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

The Formation of pH-Sensitive Wormlike Micelles in Ionic Liquids Driven by the Binding Ability of Anthranilic Acid

You

Zhang

Wang

et al. 2015

IJMS

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Wormlike micelles are typically formed by mixing cationic and anionic surfactants because of attractive interactions in oppositely charged head-groups. The structural transitions of wormlike micelles triggered by pH in ionic liquids composed of N-alkyl-N-methylpyrrolidinium bromide-based ILs (ionic liquids) and anthranilic acid were investigated. These structures were found responsible for the variations in flow properties identified by rheology and dynamic light scattering, and account for the structures observed with cryogenic transmission electron microscopy (Cryo-TEM). High-viscosity, shear-thinning behavior, and Maxwell-type dynamic rheology shown by the system at certain pH values suggested that spherical micelles grow into entangled wormlike micelles. Light scattering profiles also supported the notion of pH-sensitive microstructural transitions in the solution. Cryo-TEM images confirmed the presence of spherical micelles in the low-viscosity sample and entangled wormlike micelles in the peak viscosity sample. Nuclear magnetic resonance spectroscopy analysis revealed that the pH sensitivity of ionic liquid systems originated from the pH-dependent binding ability of anthranilic acid to the cationic headgroup of ionic liquids.

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Study on the aqueous two-phase systems composed of surfactant, ionic liquid and water

Cited by 26 publications

References 30 publications

Liquid–liquid extraction of lipase produced by psychrotrophic yeast Leucosporidium scottii L117 using aqueous two-phase systems

Liquid–liquid extraction of lipase produced by psychrotrophic yeast Leucosporidium scottii L117 using aqueous two-phase systems

Polyethylene glycol 8000+ citrate salts aqueous two-phase systems: Relative hydrophobicity of the equilibrium phases

The Formation of pH-Sensitive Wormlike Micelles in Ionic Liquids Driven by the Binding Ability of Anthranilic Acid

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