Study on the Antioxidant and Anticancer Activities of <i>Sorbus pohuashanensis (Hance) Hedl</i> Flavonoids <i>In Vitro</i> and Its Screen of Small Molecule Active Components

Yu, Xiao; Liu, Caiyan; Yang, Lianrong; Xie, Guoliang; Li, Zhengqing; Chen, Shizhe; Yang, Xiaodi; Han, Ho Jae

doi:10.1080/01635581.2021.1998560

Cited by 3 publications

(1 citation statement)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, small-molecule procyanidin B1 significantly reduced ROS levels in response to H 2 O 2 accumulation in mouse somatic cell nuclear transfer embryos [ 27 ]. Small-molecule flavonoids from Sorbus pohuashanensis were related to antitumor activity [ 28 ]. Thirdly, the intracellular antioxidants showed lower retention times than the in vitro antioxidants.…”

Section: Discussionmentioning

confidence: 99%

Revealing of Intracellular Antioxidants in Dendrobium nobile by High Performance Liquid Chromatography-Tandem Mass Spectrometry

Rao

Zhao

et al. 2023

Metabolites

View full text Add to dashboard Cite

The medicinal plant Dendrobium nobile is an important natural antioxidant resource. To reveal the antioxidants of D. nobile, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was employed for metabolic analysis. The H2O2-induced oxidative damage was used in human embryonic kidney 293T (H293T) cells to assess intracellular antioxidant activities. Cells incubated with flower and fruit extracts showed better cell survival, lower levels of reactive oxygen species (ROS), and higher catalase and superoxide dismutase activities than those incubated with root, stem, and leaf extracts (p < 0.01). A total of 13 compounds were newly identified as intracellular antioxidants by association analysis, including coniferin, galactinol, trehalose, beta-D-lactose, trigonelline, nicotinamide-N-oxide, shikimic acid, 5′-deoxy-5′-(methylthio)adenosine, salicylic acid, isorhamnetin-3-O-neohespeidoside, methylhesperidin, 4-hydroxybenzoic acid, and cis-aconitic acid (R2 > 0.8, Log2FC > 1, distribution > 0.1%, and p < 0.01). They showed lower molecular weight and higher polarity, compared to previously identified in vitro antioxidants in D. nobile (p < 0.01). The credibility of HPLC-MS/MS relative quantification was verified by common methods. In conclusion, some saccharides and phenols with low molecular weight and high polarity helped protect H293T cells from oxidative damage by increasing the activities of intracellular antioxidant enzymes and reducing intracellular ROS levels. The results enriched the database of safe and effective intracellular antioxidants in medicinal plants.

show abstract

Section: Discussionmentioning

confidence: 99%

Revealing of Intracellular Antioxidants in Dendrobium nobile by High Performance Liquid Chromatography-Tandem Mass Spectrometry

Rao

Zhao

et al. 2023

Metabolites

View full text Add to dashboard Cite

show abstract

Antioxidant and Hepatoprotective Effect of Rosa davurica Pall Seed Oil on CCl₄-Induced Acute Liver Injury in Mice

Liu,

Yu,

Zhang

et al. 2024

Journal of Medicinal Food

View full text Add to dashboard Cite

Flavonoids as Sirtuin Modulators

Deniz

Eren

Orhan

2022

CTMC

View full text Add to dashboard Cite

Sirtuins (SIRTs) are described as NAD+-dependent deacetylases, also known as class III histone deacetylases. So far, seven sirtuin genes (SIRTS 1–7) have been identified and characterized in mammals and also known to occur in bacteria and eukaryotes. SIRTs are involved in various biological processes including endocrine system, apoptosis, aging and longevity, diabetes, rheumatoid arthritis, obesity, inflammation, etc. Among them, the best characterized one is SIRT1. Actually, small molecules seem to be the most effective SIRT modulators. Flavonoids have been reported to possess many positive effects favrable for human health, while a relatively less research has been reported so far on their funcions as SIRT modulation mechanisms. In this regard, we herein aimed to focus on modulatory effects of flavonoids on SIRTs as the most common secondary metabolites in natural products. Our literature survey covering the years of 2006-2021 pointed out that flavonoids frequently interact with SIRT1 and SIRT3 followed by SIRT6. It can be also concluded that some popular flavonoid derivatives, e.g. resveratrol, quercetin, and catechin derivatives came forward in terms of SIRT modulation.

show abstract

Study on the Antioxidant and Anticancer Activities of Sorbus pohuashanensis (Hance) Hedl Flavonoids In Vitro and Its Screen of Small Molecule Active Components

Cited by 3 publications

References 24 publications

Revealing of Intracellular Antioxidants in Dendrobium nobile by High Performance Liquid Chromatography-Tandem Mass Spectrometry

Revealing of Intracellular Antioxidants in Dendrobium nobile by High Performance Liquid Chromatography-Tandem Mass Spectrometry

Antioxidant and Hepatoprotective Effect of Rosa davurica Pall Seed Oil on CCl₄-Induced Acute Liver Injury in Mice

Flavonoids as Sirtuin Modulators

Contact Info

Product

Resources

About

Study on the Antioxidant and Anticancer Activities of Sorbus pohuashanensis (Hance) Hedl Flavonoids In Vitro and Its Screen of Small Molecule Active Components

Cited by 3 publications

References 24 publications

Revealing of Intracellular Antioxidants in Dendrobium nobile by High Performance Liquid Chromatography-Tandem Mass Spectrometry

Revealing of Intracellular Antioxidants in Dendrobium nobile by High Performance Liquid Chromatography-Tandem Mass Spectrometry

Antioxidant and Hepatoprotective Effect of Rosa davurica Pall Seed Oil on CCl4-Induced Acute Liver Injury in Mice

Flavonoids as Sirtuin Modulators

Contact Info

Product

Resources

About

Antioxidant and Hepatoprotective Effect of Rosa davurica Pall Seed Oil on CCl₄-Induced Acute Liver Injury in Mice