Study on the anti-hepatocarcinoma effect and molecular mechanism of Prunella vulgaris total flavonoids

Song, Yue; Kang, Le; Tian, Shuo; Cui, Linlin; Li, Yan; Bai, Ming; Fang, Xiaoyan; Cao, Lihua; Coleman, Kimberly; Miao, Mingsan

doi:10.1016/j.jep.2021.113891

Cited by 23 publications

(16 citation statements)

References 28 publications

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“…Natural polyphenols are well evaluated as α-glucosidase inhibitory and antioxidant compounds in vitro and in vivo models, which exhibits their great potency in the treatment of type 2 diabetes [6]. Prunella vulgaris L., a plant from the Lamiaceae family, is known to be rich of polyphenols (e.g., phenolic acids and flavonoids), and the official quality marker, rosmarinic acid, is a phenolic acid derivative [7][8][9]. Prunella vulgaris L. has been widely used in traditional Chinese medicine for liver heat clearing, fire dispersing, eyesight improving, mass resolving, and swelling reduction [7].…”

Section: Introductionmentioning

confidence: 99%

“…Prunella vulgaris L. has been widely used in traditional Chinese medicine for liver heat clearing, fire dispersing, eyesight improving, mass resolving, and swelling reduction [7]. Modern pharmacological studies reveal its antioxidant, anti-diabetes, antimicrobial, anti-tumor, anti-inflammatory, and anti-HIV effects [8][9][10]. And in Asian folk medicine, P. vulgaris L. has been used for blood glucose homeostasis and antioxidant potential [10].…”

Section: Introductionmentioning

confidence: 99%

“…And in Asian folk medicine, P. vulgaris L. has been used for blood glucose homeostasis and antioxidant potential [10]. To date, investigation on P. vulgaris L. has focused on the purification and identification of chemical compounds, quantitative analysis of major compounds, activity evaluation of isolated compounds, fractions with different polarities, and crude extracts [8][9][10][11]. However, the α-glucosidase inhibitory and antioxidant polyphenolic profiling of P. vulgaris L. remains unclear.…”

Section: Introductionmentioning

confidence: 99%

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Screening of potent α‐glucosidase inhibitory and antioxidant polyphenols in Prunella vulgaris L. by bioreaction–HPLC–quadrupole‐time‐of‐flight‐MS/MS and in silico analysis

Wang

Cao

et al. 2022

J of Separation Science

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Prunella vulgaris L. is a well-known traditional Chinese medicine for blood glucose homeostasis and antioxidant potential. Ethyl acetate fraction of P. vulgaris L. demonstrated higher phenolic content (85.53 ± 6.74 mg gallic acid equivalents per gram dry weight), α-glucosidase inhibitory (IC 50 , 69.13 ± 2.86 μg/ml), and antioxidant (IC 50 , 8.68 ± 1.01 μg/ml) activities. However, the bioactive polyphenols responsible for the beneficial properties remain unclear.Here, bioreaction-HPLC-quadrupole-time-of-flight-MS/MS method was developed for rapid, accurate, and efficient screening and identification of polyphenols with α-glucosidase inhibitory and antioxidant activities from P. vulgaris L.Bioactive polyphenols can specifically bind with α-glucosidase or react with 1,1-diphenyl-2-picryl-hydrazyl radical, which was easily discriminated from nonactive compounds. Subsequently, 20 bioactive polyphenols (16 phenyl propionic acid derivatives and four flavonoids) were screened and identified. Furthermore, molecular docking analysis revealed that screened 20 polyphenols bind with the active sites of α-glucosidase through hydrogen bonding and π-π stacking. Density functional theory calculations demonstrated their electron transport ability and chemical reactivity. The in silico analysis confirmed the screened results. In summary, this study provided a valuable strategy for rapid discovering bioactive compounds from complex natural products and offered scientific evidence for further development and application of P. vulgaris L.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Screening of potent α‐glucosidase inhibitory and antioxidant polyphenols in Prunella vulgaris L. by bioreaction–HPLC–quadrupole‐time‐of‐flight‐MS/MS and in silico analysis

Wang

Cao

et al. 2022

J of Separation Science

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“…PV extract also inhibits the proliferation and migration of thyroid cancer cells both in vitro and in vivo , which may have been achieved by modulation of the expression of MKI67, PCNA, and CDH1 ( Yu et al, 2021 ). PV total flavonoids have an obvious anti-hepatocarcinoma effect, and the mechanism may be linked to the inhibition of autophagy and promotion of apoptosis in liver cancer cells, which may be related to the activation of the PI3K/Akt/mTOR pathway ( Song et al, 2021b ). Root extract of PV shows the anticancer by suppressing angiogenesis, inducing apoptosis and cell cycle arrest, whose mechanism may be via activating PI3K/AKT signaling pathway ( Gao and Xu, 2019 ).…”

Section: Biological Activitiesmentioning

confidence: 99%

Phytochemistry, Pharmacology and Quality Control of Xiasangju: A Traditional Chinese Medicine Formula

Luo

Zhong

et al. 2022

Front. Pharmacol.

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As a traditional Chinese herbal formula, Xiasangju (XSJ) is widely used in China for antipyresis and influenza treatment. However, XSJ still fails to have a comprehensive summary of the research progress in the last decade. This review summarizes the advanced research on the extraction process, phytochemistry, pharmacological activity, and quality control of XSJ. Current research mainly focuses on quality control and the pharmacological effects of single herbs and active ingredients, but many pharmacological mechanisms of the formula are unclear. The development of active ingredients reflects the active characteristics of triterpenes, phenolic acids and flavonoids, but the hepatotoxicity of Prunella vulgaris L. has not been taken into account. XSJ has extensive historical practical experiences, while systematic clinical trials remain lacking. Therefore, it is necessary to study the active ingredients and define the mechanisms of XSJ to develop multiple applications, and further studies on the dose range between its hepatoprotective activity and hepatotoxicity are necessary to improve the safety of the clinical application. In this review, the current problems are discussed to facilitate the reference basis for the subsequent research on the development of XSJ and future application directions.

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“…As natural compounds, flavonoids have attracted much attention because of their specific biological activities such as preventing cell senescence, resisting bacterial infection, and improving the body’s immunity [ 11 , 12 ]. Many medicinal mechanisms of flavonoids were closely linked to antioxidant and antibacterial activities [ 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Flavonoid Extraction from Xanthoceras sorbifolia Bunge Flowers, and the Antioxidant and Antibacterial Capacity of the Extract

Zhang

Wang

et al. 2021

Molecules

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In the present work, the extraction process of total flavonoids (TFs) from X. sorbifolia flowers by ultrasound-assisted extraction was optimized under the response surface methodology (RSM) on the basis of single-factor experiments. The optimal extraction conditions were as follows: ethanol concentration of 80%, solid–liquid ratio of 1:37 (g/mL), temperature of 84 °C, and extraction time of 1 h. Under the optimized conditions, the extraction yield of the TFs was 3.956 ± 0.04%. The radical scavenging capacities of TFs against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) were much greater than that of rutin. The results of antibacterial experiments indicated that the TFs displayed strong inhibitory activities on E. coli, S. aureus and Bacillus subtilis. Therefore, X. sorbifolia flowers can be used as a novel source of natural flavonoids, and the TFs have potential applications as natural antioxidants or antibacterial agents in the food and pharmaceutical industries.

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Study on the anti-hepatocarcinoma effect and molecular mechanism of Prunella vulgaris total flavonoids

Cited by 23 publications

References 28 publications

Screening of potent α‐glucosidase inhibitory and antioxidant polyphenols in Prunella vulgaris L. by bioreaction–HPLC–quadrupole‐time‐of‐flight‐MS/MS and in silico analysis

Screening of potent α‐glucosidase inhibitory and antioxidant polyphenols in Prunella vulgaris L. by bioreaction–HPLC–quadrupole‐time‐of‐flight‐MS/MS and in silico analysis

Phytochemistry, Pharmacology and Quality Control of Xiasangju: A Traditional Chinese Medicine Formula

Optimization of Flavonoid Extraction from Xanthoceras sorbifolia Bunge Flowers, and the Antioxidant and Antibacterial Capacity of the Extract

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