Study on the age-dependent tissue expression of FUT1 gene in porcine and its relationship to E. coli F18 receptor

Bao, Wenbin; Ye, Lan; Chen, Zi; Su, Xianmin; Pan, Zhangyuan; Zhu, Jin; Zhu, Guoqiang; Huang, Xiao-Guo; Wu, Shenglong

doi:10.1016/j.gene.2012.01.035

Cited by 10 publications

(12 citation statements)

References 19 publications

(13 reference statements)

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“…The results showed that there was a similar trend in tissue-specific expression of FUT1 gene. It expressed in all the tissues with relatively high expression in lungs, stomach, liver, duodenum and jejunum, which was in accordance with the result we've found before [12,18]). The previous researches in this field all proposed that FUT1 gene on chromosome 6 was closely linked to the E. coli F18 receptor [4,7]).…”

Section: Discussionsupporting

confidence: 92%

Expression Level of FUT1 Gene in Different Pig Populations and its Relationship with ETEC F18 Resistance

Bao¹

2015

SOJVS

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SOJ Veterinary Sciences Open Access Research Article α(1,2)-fucosyltransferase exhibiting α(1,2)-fucosylation of glycolipid and glycoprotein acceptors has been purified from submaxillary gland mucin [4]. Thurin and Blaszczyk-Thurin M [5] identified this enzyme as the homologue of the human Secretor enzyme. Recent studies indicated that FUT1 gene was important in the synthesis of the structure that was beneficial to the adhesion between E. coli F18 fimbriated bacteria and the small intestinal wall [6]. By linkage analysis, Meijerink, et al.[7] estimated that the FUT1 gene polymorphism was less than 1 centimorgan from the S and E. coli F18 receptor loci. Therefore, FUT1 gene was regarded as a good candidate for gene controlling the expression of the receptor for E. coli F18 bacteria. Sequencing of the FUT1 gene revealed a polymorphism (G or A) at nucleotide 307 resulting in an amino acid Ala being substituted for Thr at position 103. The E. coli F18-resistant pigs showed presence of the a nucleotide on both alleles (AA genotype), whereas pigs susceptible to E. coli F18 had either the heterozygous AG genotype or the homozygous GG genotype [7,8]. However, previous investigations conducted by Chinese scientists have shown that the polymorphism of FUT1 gene at nucleotide 307 only displays in foreign pig breeds and hybrid lines bred with foreign lineages such as the Sutai pig. There was no AA genotype or even the AG genotype in most Chinese domestic pig breeds, except the Lingao pig breed which carried a small proportion of AG genotype [9-11]. The Sutai pig is a new hybrid between the Duroc and Taihu breeds that produces high-quality lean meat. In previous studies, we identified a few FUT1 AG animals (9.2%) in a Sutai pig population and selectively bred them to generate the prized Sutai FUT1 AA individuals (ETEC F18 resistant). After five years of continuous selection and breeding, the E. coli F18-resistant resource population with AA genotype was established [12]. Simultaneously, we also constructed a type V secretion system to express ETEC F18 adhesin. The display of functional adhesin through the type V secretion system was combined with receptor binding experiments to further analyze and verify the resistance Abstract F18-fimbriated Escherichia coli are associated with porcine post weaning diarrhea and edema disease. Some pigs show inherent resistance to F18 ETEC infection and this is associated with a G/A mutation at position M307 of the alpha (1,2)-fucosyltransferase (FUT1) gene. Pigs with genotype AA are resistant to ETEC F18 and pigs with genotype GG or AG are susceptible to ETEC F18 infection. So the M307 of FUT1 gene has been proposed as a genetic marker to distinguish the E. coli F18 resistant from susceptible phenotypes in some imported pigs. The objective of this study was to investigate the different expression levels of FUT1 among Yorkshire, Meishan, and E. coli F18-resistant Sutai groups, especially in Meishan. The results showed that FUT1 was expressed consistently in 11 tissues in the three populations...

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Section: Discussionsupporting

confidence: 92%

Expression Level of FUT1 Gene in Different Pig Populations and its Relationship with ETEC F18 Resistance

Bao¹

2015

SOJVS

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“…Zhao et al (2014) analyzed TAP1 mRNA relative expression levels in 11 tissues from resistant and susceptible phenotypes, and found that the G729A mutation of TAP1 had a significant effect on TAP1 mRNA expression, with high expression possibly conferring resistance against E. coli F18, which confirms that the TAP1 gene plays an important role in E. coli F18 resistance (Wang et al, 2014). These results are also in agreement with Bao et al (2012).…”

Section: Polymorphism Of the Tap1 Genesupporting

confidence: 65%

“…Recently, some researchers have reported that TAP1 may be an effective anti-E. coli F18 molecular marker in pigs (Ambagala et al, 2003). Bao et al (2012) detected genetic variations in exon 3 of the TAP1 gene and evaluated TAP1 mRNA expression levels among the different genotypes, and found the G729A mutation had a significant effect on mRNA expression of TAP1, with individuals with the GG genotype possessing a stronger ability to resist E. coli F18 infection.…”

Section: Introductionmentioning

confidence: 99%

Analysis of polymorphisms in the FUT1 and TAP1 genes and their influence on immune performance in Pudong White pigs

Zhang

Wang

Xq³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. FUT1 and TAP1 have been identified as candidate genes that offer resistance against Escherichia coli F18 infection, with the AA genotype in FUT1 and the GG genotype in TAP1 conferring resistance. In order to confirm polymorphisms at FUT1 M307 and TAP1 G729, and evaluate their influence on immunity performance in Pudong White pigs, we performed polymerase chain reaction-restriction fragment length polymorphism analysis, measured immune indices, and compared the results with those observed in Large White pigs. The AA genotype of FUT1 was first discovered in Pudong White pigs and has not been found in other Chinese domestic pig breeds. The frequency of the AA genotype in Pudong White and Large White pigs was 0.018 and 0.052, respectively. The GG genotype of TAP1 was also detected in the two breeds, with a frequency of 0.708 and 0.695, respectively. Chi-square fitness analysis of both genes showed that these loci deviated from Hardy-Weinberg equilibrium in the two breeds (P < 0.05). No significant differences were observed in (2015) interleukin-6 (IL-6) and IL-10 levels among the three genotypes at FUT1 and TAP1 in the two breeds (P > 0.05). Individuals for all genotypes of TAP1 in both pig breeds had similar TNF-α levels (P > 0.05), implying that Pudong White pigs may have the same ability for hepatocyte inflammatory response and B cell differentiation as Large White pigs. These differences have a degree of influence on Pudong White pig's immune ability to resist F18 or other infections.

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“…Indeed, genome wide associate studies have associated the mutation in the FUT2 gene to the risk of developing CD and UC [ 2 – 3 ]. In pigs, both the FUT1 and FUT2 genes are expressed in the intestines and the FUT1 is considered to be orthologous to the human H gene which catalyses the addition of fucose to a terminal galactose in an alpha 1,2-linkage [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of alpha-(1,2)-fucosyltransferase genotype variants on plasma metabolome, immune responses and gastrointestinal bacterial enumeration of pigs pre- and post-weaning

et al. 2018

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In pigs, the alpha-(1,2) fucosyltransferase (FUT1) gene has been highlighted for its properties in controlling the intestinal expression of enterotoxigenic E. coli (ETEC) F18 receptors; a pathogen causing edema disease and post-weaning diarrhoea. In this study, we hypothesized that pigs with different genotypes (ETEC F18 resistant (FUT1AA) versus susceptible (FUT1AG)) differed in following systemic and enteric responses: growth performance, plasma metabolic profiles, expression of candidate genes for intestinal mucosal homeostasis and immunity, number of selected bacteria and the concentration of short-chain fatty acids (SCFA) in faeces and digesta in piglets pre and post-weaning, and on the ETEC F18 adherence ex vivo. Genotype had the strongest impact on plasma metabolomic profile on day 7 and 28 of age. FUT1AG piglets had higher level of N-methyl-2-pyrrolidinone, hippuric acid, oxindole, and 3-oxo-5-beta-chol-7-en-24-oic acid on day 7, and a higher level of guanosine on day 28 than that in the FUT1AA piglets. FUT1AA piglets had a higher level of betaine on day 7 and 3-methylguanine on day 28. On day 34 of age, the FUT1AA pigs had higher levels of S-2-hydroxyglutarate, L-phenylalanine, tauroursodeoxycholic acid and an undetermined PC/LysoPC, while Ile Glu Phe Gly peptide and genistein 5-O-glucuronide, and PC (18:0/0:0) were at higher levels in the FUT1AG piglets. FUT1 genotype did not affect the growth performance and expression of candidate genes. FUT1AG piglets had a higher number of haemolytic bacteria in faeces and in digesta than that in FUT1AA at 34 days of age. The colonic acetic acid concentration was highest in FUT1AG piglets. FUT1 genotype may influence not only the expression of E. coli F18 receptors but could potentially impact the gut homeostasis and metabotype of piglets pre and post-weaning. Further investigations on the relation between FUT1 genotype and these aspects including the intestinal commensal microbiota will expand the knowledge on factors affecting the intestinal ecosystem.

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Study on the age-dependent tissue expression of FUT1 gene in porcine and its relationship to E. coli F18 receptor

Cited by 10 publications

References 19 publications

Expression Level of FUT1 Gene in Different Pig Populations and its Relationship with ETEC F18 Resistance

Expression Level of FUT1 Gene in Different Pig Populations and its Relationship with ETEC F18 Resistance

Analysis of polymorphisms in the FUT1 and TAP1 genes and their influence on immune performance in Pudong White pigs

Effects of alpha-(1,2)-fucosyltransferase genotype variants on plasma metabolome, immune responses and gastrointestinal bacterial enumeration of pigs pre- and post-weaning

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