Study on the ability of producing of conjugated Linoleic acid of Lactobacillus fermentum A01 isolated from human digestive tract

Anh, Nguyen Pham Quynh; Yen, Tran Thi Hai; Vinh, Doan Thi Thanh; Tien, Tran Phu; Dũng, Nguyễn Anh; Tu, Nguyen Hoang Khue

doi:10.5958/0974-360x.2021.00234.1

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Microorganisms produced many biological compounds such as bacteriocin, conjugated linoleic acid, gamma buryric acid, lactic acid from lactic acid bacteria [1][2][3][4] , prodigiosin production from Streptomyces coelicolor 5 , penicillin from fungi 6 . Defined as an organism growing, existing within the plant causing no harm to its host, the endophyte is known to generate no apparent symptoms of disease and some of natural plant compounds 7 .…”

Section: Introductionmentioning

confidence: 99%

Detection of Antimicrobial, Antioxidant and Cytotoxicity Activities of Fusarium oxysporum F01 Isolated from Catharanthus roseus Collected in Vietnam

Tu¹,

Thuy²,

Vinh³

et al. 2021

J. Pure Appl. Microbiol.

Self Cite

View full text Add to dashboard Cite

The ability of endophytic fungi to produce valuable bioactive compounds when surviving in medicinal herbs. Finding out endophytic fungi originated from Catharanthus roseus with antimicrobial, antioxidant and cytotoxicity activities is important for pharmaceutical development. The isolation was based on the morphology of fungi. Identification was performed by sequencing 18S rRNA that was compared with known genes using Blast search in the combination of phylogenic analysis by using Clustal W and PhyML in GenomeNet. For the antimicrobial test, the agar diffusion method was used. DPPH scavenging assay for the antioxidant activity determination by using spectrophotometry. The cytotoxicity test was carried out by Sulforhodamine B method. LC-MS was applied for predicting components. We isolated the Fusarium oxysporum F01 strain originated from Catharanthus roseus. The aqueous extracts showed inhibition against Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853 (15.00 ± 0.50 mm), Serratia marcescens ATCC 14756 (9.00 ± 0.87 mm), Vibrio parahaemolyticus ATCC 17802 (15.50 ± 0.87 mm), Escherichia coli ATCC 25922 (12.50 ± 0.50 mm). The antioxidant activity of the extract obtained from the supernatant was determined with an IC50 value of about 11 µg/mL. The extract also showed cytotoxicity effect on liver cancer cell line (HepG2) and breast cancer cell line (MCF-7) with the percentage of inhibition of 84.47 ± 3.18 and 94.69 ± 1.59, respectively. LC-MS was used to point out the presence of pratol, a melanogenesis agent. The study provided more interesting information about Fusarium oxysporum F01 isolated in Catharanthus roseus grown in Vietnam, contributing to pharmaceutical sources in the world.

show abstract