Study on spray-drying of Bacillus velezensis NKMV-3 strain, its formulation and bio efficacy against early blight of tomato

Vignesh, Murthy; Shankar, S. R. Madhan; Subramani, Nagaraj; VedhaHari, Bodethala Narayanan; Durai, Ramya Devi

doi:10.1016/j.bcab.2022.102483

Cited by 3 publications

(4 citation statements)

References 40 publications

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“…Five hundred milliliter Erlenmeyer flasks, containing 200 mL of sterile Soy based medium (15 g/L soybean flour, 5 g/L dextrose, 5 g/L cornstarch, 1 g/L K 2 HPO 4 , 1 g/L of KH 2 PO4, 0.3 g/L MgSO 4 *7H 2 O, 0.02 g/L FeSO 4 *7H 2 O, 0.02 g/L ZnSO 4 *7H 2 O and 1 g/L CaCO 3 ; pH: 7) were aseptically inoculated with a single bacterial colony obtained from a Petri dish. The flasks were incubated at 37°C, 200 rpm, during 72 h. Finishing the incubation period, a heat treatment was employed by subjecting the culture to 80°C for 15 min to eliminate selectively vegetative cells, following the procedure described by Vignesh et al (2022) .…”

Section: Methodsmentioning

confidence: 99%

“…Subsequently, the endospore suspension was added with 10% (w/v) of maltodextrin as a carrier ( Bhagwat et al, 2020 ; Vignesh et al, 2022 ) and dried using a laboratory spray dryer (Yamato ADL3115) at 110°C and 75°C as inlet and outlet air temperature, respectively, obtaining a fine powder containing around 1 × 10 8 endospores/g. Endospores were quantified, resuspending the powder in water and employing two methods: (a) using a Newbauer chamber (40X); (b) by plate count, according to Bolanos-Carriel and Vazquez Soto (2022) method.…”

Section: Methodsmentioning

confidence: 99%

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Native Bacillus paralicheniformis isolate as a potential agent for phytopathogenic nematodes control

Chavarria-Quicaño,

Contreras-Jácquez,

Carrillo-Fasio

et al. 2023

Front. Microbiol.

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Phytopathogenic nematodes (PPNs) are responsible for substantial damages within agricultural crops worldwide which can be controlled employing beneficial microorganisms and/or their metabolites in an ecofriendly way. Nevertheless, the success of the control regards not only on the virulence of the strains or the toxicity of their metabolites but also on their ability to colonize and remain in the rhizospheric environment, particularly in those crops affected by abiotic stresses promoted by the climate change. Consequently, the bioprospection of beneficial microorganisms able to control PPNs and to thrive in adverse conditions has attracted attention. On this way, deserts are perfect ecological niches to isolate microorganisms adapted to harsh enviroments. The purpose of this research was to isolate and characterize bacteria from rhizospheric soil samples collected in the Northwestern Desert of Mexico with potential for PPNs control. As first screening, secretomes of each isolate were tested in vitro for nematicidal activity (NA). Then, activities from secretomes and endospores from the selected isolate were confirmed in vivo assays. From 100 thermotolerant isolates, the secretome of the isolate identified as Bacillus paralicheniformis TB197 showed the highest NA (>95%) against Meloidogyne incognita, both in vitro and in vivo tests, suppressing infections caused by M. enterolobii in tomato crops, too. In open field tests, the endospores of TB197 strain showed a reduction of 81% in the infection severity caused by M. enterolobii (p ≤ 0.05), while the galling index (GI) was reduced 84% (p ≤ 0.05) in tomato greenhouse-tests. Also, a reduction of the root necrosis (81%) caused by Radopholus similis in banana plantations (p ≤ 0.05), compared to the control was observed. Owing to their efficacy in controlling PPNs infections, the endospores and secondary metabolites of B. paralicheniformis TB197 strain could be used in bionematicidal formulations.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Native Bacillus paralicheniformis isolate as a potential agent for phytopathogenic nematodes control

Chavarria-Quicaño,

Contreras-Jácquez,

Carrillo-Fasio

et al. 2023

Front. Microbiol.

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“…Moreover, foliar application of both strains individually and in a mixture significantly reduced the incidence of grey mould on tomatoes in greenhouse conditions. Strain B. velezensis NKMV-3, along with its lipopeptide extract, consisting of surfactin, iturin, and fengycin, effectively controlled A. solani on tomatoes in greenhouse studies [ 91 ]. Jia et al [ 92 ] found that B. amyloliquefaciens XJ-BV2007 produces fengycin, which has an important role in the control of black spot disease of tomato and mycotoxins caused by A. alternata .…”

Section: Mechanisms Of Biological Controlmentioning

confidence: 99%

Bacillus Species: Excellent Biocontrol Agents against Tomato Diseases

Karačić,

Miljaković,

Marinković

et al. 2024

Microorganisms

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Tomatoes encounter many pathogens, such as fungi and bacteria, which reduce the yield and quality of plants and lead to large losses in production. The application of plant protection products (PPPs) is still an important and most effective measure to control plant diseases. However, the use of chemicals in agriculture contributes to environmental pollution and biodiversity loss, and it can also threaten non-target living organisms. Biological control is a widely accessible, environmentally safe, and cost-efficient alternative for the prevention and suppression of plant diseases. Bacillus species with antimicrobial and plant growth-promoting effects are most frequently used as biocontrol agents to increase the resilience of agricultural production against biotic stresses. The present review discusses the antagonistic mechanisms and the biocontrol potential of Bacillus spp. against tomato diseases caused by different pathogens. The main mechanisms of Bacillus spp. include the production of antimicrobial compounds (antibiotics, extracellular enzymes, siderophores, and volatile compounds), competition for nutrients and space, and induced systemic resistance (ISR). Although Bacillus-based PPPs have been developed and commercialised worldwide for various crops and pathogens, the efficiency issues are still subject to debate. Additionally, a combined strategy for controlling tomato diseases based on Bacillus spp. and other available methods (conventional or natural-based) is a promising research field.

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“…Nowadays, Bacillus-based products, also engineered to improve lipopeptide yield [21], are commercially available as biocontrol agents against phytopathogenic fungi and are employed directly to the soil or sprayed in the plant surface [22,23]. In the present work, a commercial Bacillus subtilis QST 713 was studied for its ability to produce different lipopeptides and inhibit the presence of some of the main mycotoxigenic fungi as well as their regulated mycotoxins.…”

Section: Introductionmentioning

confidence: 99%

Reduction of Mycotoxigenic Fungi Growth and Their Mycotoxin Production by Bacillus subtilis QST 713

Bertuzzi

Bulla

Giorni

2022

Toxins

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The use of chemical pesticides to control the occurrence of mycotoxigenic fungi in crops has led to environmental and human health issues, driving the agriculture sector to a more sustainable system. Biocontrol agents such as Bacillus strains and their antimicrobial metabolites have been proposed as alternatives to chemical pesticides. In the present work, a broth obtained from a commercial product containing Bacillus subtilis QST 713 was tested for its ability to inhibit the growth of mycotoxigenic fungi as well as reduce their mycotoxin production. Mass spectrometry analysis of Bacillus subtilis broth allowed to detect the presence of 14 different lipopeptides, belonging to the iturin, fengycin, and surfactin families, already known for their antifungal properties. Bacillus subtilis broth demonstrated to be a useful tool to inhibit the growth of some of the most important mycotoxigenic fungi such as Aspergillus flavus, Fusarium verticillioides, Fusarium graminearum, Aspergillus carbonarius, and Alternaria alternata. In addition, cell-free Bacillus subtilis broth provided the most promising results against the growth of Fusarium graminearum and Alternaria alternata, where the radial growth was reduced up to 86% with respect to the untreated test. With regard to the mycotoxin reduction, raw Bacillus subtilis broth completely inhibited the production of aflatoxin B1, deoxynivalenol, zearalenone, and tenuazonic acid. Cell-free broth provided promising inhibitory properties toward all of the target mycotoxins, even if the results were less promising than the corresponding raw broth. In conclusion, this work showed that a commercial Bacillus subtilis, characterized by the presence of different lipopeptides, was able to reduce the growth of the main mycotoxigenic fungi and inhibit the production of related mycotoxins.

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Study on spray-drying of Bacillus velezensis NKMV-3 strain, its formulation and bio efficacy against early blight of tomato

Cited by 3 publications

References 40 publications

Native Bacillus paralicheniformis isolate as a potential agent for phytopathogenic nematodes control

Native Bacillus paralicheniformis isolate as a potential agent for phytopathogenic nematodes control

Bacillus Species: Excellent Biocontrol Agents against Tomato Diseases

Reduction of Mycotoxigenic Fungi Growth and Their Mycotoxin Production by Bacillus subtilis QST 713

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