Study on Risk Factors for Transplacental Viral Infections; Effect of Bacterial Factors and Double Viral Infections on Virus Replication in Placenta and Amniotic Membranes

Jatczak, Bogna; E, Gejdel; Pajk, J.; Podwińska, J; Błach-Olszewska, Z

doi:10.1053/plac.2000.0623

Cited by 8 publications

(6 citation statements)

References 14 publications

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“…Similarly, effects depending on the level of innate immunity were noticed following culture treatments with immunomodulating substances, i.e. lipopolysaccharide and peptides from ovine colostrums [ 5 , 9 ]. The virus stimulatory effect, however, was eliminated by antibodies against TNF-α.…”

Section: Discussionmentioning

confidence: 99%

Cytokine production by human leukocytes with different expressions of natural antiviral immunity and the effect of antibodies against interferons and TNF-±

Orzechowska

Antoszków

Siemieniec

et al. 2007

Arch. Immunol. Ther. Exp.

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Introduction: Two activities of innate antiviral immunity were studied: the resistance of human peripheral blood mononuclear cells (PMBCs) ex vivo to viral infection and the production of cytokines. Materials and Methods: Samples of blood were taken from healthy blood donors and from persons with frequent infections of the upper respiratory system. PMBCs were isolated by gradient centrifugation. Vesicular stomatitis virus (VSV) was used as the indicatory virus to infect PMBCs. The cytokines: IFN, TNF, and IL-6 were titrated by biological methods and IL-10 by ELISA. Results: Blood donors were divided for two groups: those with VSV-resistant and those with VSV-sensitive PMBCs and secretion of cytokines by them was compared. The resistant PMBCs produced more cytokines than the sensitive ones. A statistically significant difference, was found only in the case of the IFNs. To examine the contribution of IFNs and TNF in maintaining resistance, leukocytes from both groups were treated with specific anti-cytokine antibodies. The authors' previous study showed that the elimination of spontaneous IFN-α, IFN-β, IFN-γ, and TNF-α from resistant leukocytes resulted in increased VSV replication This indicates the important role of cytokines. In VSV-sensitive PMBCs, anti-IFN-α showed the opposite effect (decreased virus replication). In the absence of spontaneous IFN-α, disturbances in cytokine production were observed. Conclusions: Complete resistance of PMBC to VSV infection is accompanied by higher cytokine release, The paradoxical effect of anti-IFN-α on virus replication in leukocytes sensitive to viral infection may be attributed to changes in the cytokine profile balance, i.e. high TNF production by VSV-infected leukocytes and a complete reduction of IL-6 production.

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Section: Discussionmentioning

confidence: 99%

Cytokine production by human leukocytes with different expressions of natural antiviral immunity and the effect of antibodies against interferons and TNF-±

Orzechowska

Antoszków

Siemieniec

et al. 2007

Arch. Immunol. Ther. Exp.

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“…18,23,26 Pregnant women without available maternal or cord blood data were also excluded from the final analysis. 18,23,26 Pregnant women without available maternal or cord blood data were also excluded from the final analysis.…”

Section: Case Definitionsmentioning

confidence: 99%

Immunoprophylaxis Failure Against Vertical Transmission of Hepatitis B Virus in the Chinese Population

Lin

Guo

Zhou

et al. 2014

Pediatric Infectious Disease Journal

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“…Different studies indicate that maternal immunity, gestational age of maternal infection, timing of intrauterine HCMV transmission, infection by a new strain of HCMV and infection by special genotypes of HCMV are determinants of intrauterine transmission of HCMV (24–28). It had also been observed that inhibition and stimulation of virus replication are dependent on the level of innate antiviral immunity (29–31). Rapid detection and quantification of viral DNA in the placenta may play an important role in the diagnosis of congenital HCMV infection and lead to the improved identification of newborns at risk of severe complications.…”

Section: Discussionmentioning

confidence: 99%

Detection of cytomegalovirus in human placental cells by polymerase chain reaction

Paradowska

Przepiórkiewicz

Nowakowska

et al. 2006

APMIS

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Human cytomegalovirus (HCMV) is the most common cause of viral intrauterine infection. Progress in rapid, specific, and dependable detection of HCMV has recently been achieved by the use of DNA hybridization techniques and other molecular methods. We examined 21 placentas after delivery for the presence of HCMV DNA by polymerase chain reaction (PCR). To test the reliability of the PCR for the detection of HCMV DNA in clinical specimens, two simple PCR assays and a real-time quantitative PCR were used. PCR analysis of villous and decidual cells showed that HCMV DNA was present in 16 placentas (76.2%). Transmission of HCMV infection to chorionic villi was confirmed in 11 organs (52.4%), and congenital infections in newborns were detected in 9 cases (42.8%). These results suggest that HCMV genome detection in placentas at later gestational ages is common. Our results demonstrated that detection of HCMV DNA in placental tissues by DNA amplification provides a specific and sensitive method for diagnosis of intrauterine HCMV infection.

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Study on Risk Factors for Transplacental Viral Infections; Effect of Bacterial Factors and Double Viral Infections on Virus Replication in Placenta and Amniotic Membranes

Cited by 8 publications

References 14 publications

Cytokine production by human leukocytes with different expressions of natural antiviral immunity and the effect of antibodies against interferons and TNF-±

Cytokine production by human leukocytes with different expressions of natural antiviral immunity and the effect of antibodies against interferons and TNF-±

Immunoprophylaxis Failure Against Vertical Transmission of Hepatitis B Virus in the Chinese Population

Detection of cytomegalovirus in human placental cells by polymerase chain reaction

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