Study on Process Optimization and Antioxidant Activity of Polysaccharide from Bletilla striata Extracted via Deep Eutectic Solvents

Abstract

Taking the extraction yield of Bletilla striata polysaccharide (BSP) as the index and taking the type of deep eutectic solvents (DESs), extraction time, extraction temperature, DES water content, and solid–liquid ratio as the investigation factors, single-factor and Box–Behnken response surface tests were carried out to optimize the extraction process of BSP. Thus, the antioxidant activity of BSP on DPPH radicals, ABTS radicals and ferric reducing antioxidant power were determined. The results showed that the most suitable deep eutectic solvent was DES-2, namely choline chloride-urea. The optimal extraction conditions for BSP were an extraction time of 47 min, extraction temperature of 78 °C, water content of 35%, and solid–liquid ratio of 1:25. Under this optimized condition, the extraction yield of BSP was able to reach (558.90 ± 8.83) mg/g, and recycling studies indicated the good cycle stability of the DES. Antioxidant results showed that BSP had superior antioxidant activity and had a dose–response relationship with drug concentration. Compared with Bletilla striata polysaccharide obtained via conventional hot water extraction (BSP-W), the extraction yield of BSP obtained through this method (BSP-2) increased by 36.77%, the scavenging activity of DPPH radicals increased by 24.99%, the scavenging activity of ABTS radicals increased by 41.16%, and the ferric reducing antioxidant power increased by 49.19%. Therefore, DESs as new green reagents and BSP extracted with DESs not only had a high yield but also had strong antioxidant activity.

Valorization of biomass for food protein via deep eutectic solvent extraction: Understanding the extraction mechanism and impact on protein structure and properties

“…However, it is important to recognize that the co-extraction of phenolic compounds may also have an impact on the antioxidant activity. DESs have been shown to be efficient at extracting nonprotein components with potential for bioactivity, such as polysaccharides and polyphenols (Luo et al, 2023;Redha, 2021).…”

Optimization of an Ultrasound-Assisted Extraction Technique and the Effectiveness of the Sunscreen Components Isolated from Bletilla striata

“…The B. striata plant is mainly distributed in the humid river valleys and coastlands in the most southeastern three provinces of China. Outside China, its global distribution is limited, only growing in the Korean Peninsula, Japan and Myanmar [ 8 ]. Traditionally, it has been used for hemostasis therapy, as well as to treat sores, swelling and chapped skin [ 9 ].…”

“…Nowadays, some regions of China still retain this ancient method of skincare [ 10 ]. The main chemical components of B. striata consist of a variety of chemical substances, such as polysaccharides, benzenes, dihydrophenanthrolines and phenanthrenes, flavonoids, diphenylenes and diphenylene ethers and quinones [ 8 , 11 ]. Therefore, B. striata has a wide range of bio-pharmacological activities, such as bacteriostasis, anti-inflammatory [ 12 ], hemostasis [ 13 ], anti-tumor [ 14 ], cell-growth-promoting and wound healing properties [ 11 , 15 ].…”

“…Therefore, B. striata has a wide range of bio-pharmacological activities, such as bacteriostasis, anti-inflammatory [ 12 ], hemostasis [ 13 ], anti-tumor [ 14 ], cell-growth-promoting and wound healing properties [ 11 , 15 ]. Additionally, it has been shown to have a significant antioxidant effect in vitro [ 8 , 16 , 17 ]. Most of the chemical structures of B. striata compounds contain benzene ring skeletons and even multiple phenolic hydroxyl groups.…”

A New Method of Extracting Polygonatum sibiricum Polysaccharide with Antioxidant Function: Ultrasound-Assisted Extraction-Deep Eutectic Solvents Method

“…Moreover, the internal structure of DESs can form hydrogen bonds with target polysaccharides and create electrostatic interactions, significantly improving the extraction efficiency of polysaccharides [ 20 ]. More and more studies have shown that the extraction with DESs of plant active components can not only significantly improve the extraction yield, but also retain the functional activity of the active substances [ 21 , 22 ]. DESs are characterized by their low surface tension and viscosity, which enable them to penetrate into polysaccharides more effectively and enhance extraction efficiency.…”