Study on porous silk fibroin materials. I. Fine structure of freeze dried silk fibroin

Li, Mingzhong; Lu, Shenzhou; Wu, Zhengyu; Yan, Haojing; Mo, Jingyu; Wang, Lihong

doi:10.1002/1097-4628(20010321)79:12<2185::aid-app1026>3.0.co;2-3

Cited by 139 publications

(91 citation statements)

References 18 publications

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“…The different crystalline states were detected by FTIR spectra, resulting in a typical shift for amide III bond stretching (1235 and 1265 cm − 1 ) and changes in the amide V bond regions. In particular the shoulder in the amide III band region is indicative for a conformational shift of silk fibroin into β-sheet structure, as described before [31,43]. Changes in silk assembly as a result from a silk I to a silk II conformational change were further detailed by wide angle X-ray scattering (WAXS) before and after methanol treatment.…”

Section: Discussionmentioning

confidence: 89%

Silk fibroin as an organic polymer for controlled drug delivery

Hofmann

Foo

Rossetti

et al. 2006

Journal of Controlled Release

338

245

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Section: Discussionmentioning

confidence: 89%

Silk fibroin as an organic polymer for controlled drug delivery

Hofmann

Foo

Rossetti

et al. 2006

Journal of Controlled Release

338

245

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“…The SF Raw (Figure 8a) displayed a strong absorption band at 3239 cm -1 (NHstretching), assigned to the hydrogen bonded NH groups, in addition to the characteristic amide bands at 1698 and 1631 cm -1 (amide I), 1518 cm -1 (amide II), 1263 and 1231 cm -1 (amide III) assigned to the β sheet, as described elsewhere 44,45 . Spectra shows strong twin bands at 996 and 976 cm -1 however no bands at 1015 and 965 cm -1 which can be conclude that in the part of silk fibroin the glycine and alanine residues are arranged alternately just as in periodic sequence 46 .…”

Section: Spectroscopic Characterization Of Sf Fibersmentioning

confidence: 52%

Fabrication and characterization of nanofibrous scaffold developed by electrospinning

Dhandayuthapani¹,

Yasuhiko²,

Maekawa³

et al. 2011

Mat. Res.

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Electrospinning has been recognized as an efficient technique for the forming of polymer nanofibers. Silk fibroin (SF) nanofibers were electrospun from SF solution using trifluoroacetic acid solution as a solvent. In the present work, we have systematically evaluated the effects of instrument parameters, including applied voltage, tip-target distance, solution flow rate, solution parameters; such as polymer concentration and solution viscosity on the morphology of electrospun SF fibers. The applied voltage and flow rate was monitored at fixed tip target distance during the electrospinning process and it was correlated with the characteristics of the fibers obtained. The number of deposited fibers also increases with the applied voltage. Also, viscosity, flow rate and applied voltage strongly affect the shape and morphology of the fibers. A particular interest, we demonstrated that by monitoring the applied voltage and flow rate it is possible to control the fibers morphology and bead concentration. Rheological study showed a strong dependence of spinnability and fiber morphology on solution viscosity. Solution concentrations has been found to most strongly affect fiber size, with fiber diameter increasing with increasing solution concentration and the morphology of the deposition on the collector changed from spherical beads to interconnected fibrous networks. FTIR analysis clearly shows that there are no spectral differences between fibers and which suggests that there was no chemical modification developed during the process. Under optimized conditions, homogenous (not interconnected) SF fibers with a mean diameter of 234 nm were prepared.

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“…Figure 7 shows Xray profiles of lyophilized hydrogels prepared from SF aqueous solutions. When silk fibroin solutions freeze at low temperature below the glass transition from −34 to -20°C, the structure doesn't change significantly (Li et al, 2001). The amorphous structure of lyophilized silk fibroin hydogel samples were indicated by exhibition of a broad peak at around 20° (Magoshi et al, 1996).…”

Section: Structural Analysismentioning

confidence: 99%

Preparation, characterization and in vitro study of biocompatible fibroin hydrogel

Sah¹,

Pramanik²

2011

Afr. J. Biotechnol.

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In this study, Bombyx mori silk based hydrogels were prepared and their biorelevant properties like physical, chemical and thermal properties were studied. Firstly, silk fibroin aqueous solution was prepared and the molecular weight of fibroin protein was determined followed by particle size analysis for the confirmation of study. Silk fibroin hydrogels were prepared by treating a 12% (w/v) silk fibroin aqueous solution at 4°C (thermgel) and lyophilized. The swelling and thermorheological behaviour of fibroin hydrogels were studied. The morphology and crystalline structure of lyophilized hydrogels were investigated by scanning electron microscopy (SEM) and wide-angle diffractometry, respectively while the surface functional groups were analyzed by FT-IR. The thermal behavior was also studied by means of differential scanning calorimetry and gravimetric method. The cytocompatibility of the hydrogels was evaluated through three-dimensional culture with human peripheral blood mononuclear cells. Lyophilized fibroin gel of high strength and high thermal stability were obtained. The β-crystelline structure of lyophilized fibroin hydrogel has shown excellent swelling capacity to mimic the living tissues. The surfaces of these hydrogels were found supporting to cell adherence and proliferation. hMNCs could survive and proliferate in the gel within 3 weeks, and the gel had good cytocompatibility. It was concluded that fibroin hydrogel not only has interpenetrating network structure but also has good cytocompatibility and could be used as injectable scaffolds able to promote in situ bone regeneration.

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Study on porous silk fibroin materials. I. Fine structure of freeze dried silk fibroin

Cited by 139 publications

References 18 publications

Silk fibroin as an organic polymer for controlled drug delivery

Silk fibroin as an organic polymer for controlled drug delivery

Fabrication and characterization of nanofibrous scaffold developed by electrospinning

Preparation, characterization and in vitro study of biocompatible fibroin hydrogel

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