With the further development of pharmaceutical research and the advancement of instrumental determination technology, pharmaceutical analysis is not only the analysis, inspection and control of drug quality, but also its research and teaching contents have been extended to all aspects of pharmaceutical research, and it plays a key role. In this paper, the new situation of the development of animal pharmacy at home and abroad in recent years, the course characteristics and teaching mode of drug analysis are analyzed. The improvement measures were put forward to improve the teaching quality and cultivate competitive talents of animal pharmacy who can promote the healthy development of the animal protection industry. In this paper, through the teaching form of Mu class, teaching the course of animal pharmacy analysis. And from the characteristics of resource sharing, new learning methods of learners, the reform of teaching mode of animal drug analysis, the change of teachers' ideas in the new era, etc, summarizes the positive influence of the teaching mode of Mu Course, and finally analyses the challenges faced by the current construction of Mu Course. In order to promote the promotion of Mu class teaching in the classroom of animal drug analysis. The experiment was designed to compare the teaching results of animal pharmacy analysis under the traditional teaching mode and the Mu-class teaching mode. The experimental results prove that the teaching mode of Mu class has high feasibility in teaching practice. As a supplementary part of traditional teaching, the Mu-class teaching mode exists, combining "self-directed learning" with "self-directed learning" to change the situation of unification of single traditional teaching mode, it is more conducive to students' learning and development.