Study on Paraffin Section Technology of Fruit Tumor Tissues in Cucumber (&lt;i&gt;Cucumis sativus&lt;/i&gt; L.)

Stem growth and development has considerable effects on plant architecture and yield performance. Strigolactones (SLs) modulate shoot branching and root architecture in plants. However, the molecular mechanisms underlying SLs regulate cherry rootstocks stem growth and development remain unclear. Our studies showed that the synthetic SL analog rac-GR24 and the biosynthetic inhibitor TIS108 affected stem length and diameter, aboveground weight, and chlorophyll content. The stem length of cherry rootstocks following TIS108 treatment reached a maximum value of 6.97 cm, which was much higher than that following rac-GR24 treatments at 30 days after treatment. Stem paraffin section showed that SLs affected cell size. A total of 1936, 743, and 1656 differentially expressed genes (DEGs) were observed in stems treated with 10 μM rac-GR24, 0.1 μM rac-GR24, and 10 μM TIS108, respectively. RNA-seq results highlighted several DEGs, including CKX, LOG, YUCCA, AUX, and EXP, which play vital roles in stem growth and development. UPLC-3Q-MS analysis revealed that SL analogs and inhibitors affected the levels of several hormones in the stems. The endogenous GA3 content of stems increased significantly with 0.1 μM rac-GR24 or 10 μM TIS108 treatment, which is consistent with changes in the stem length following the same treatments. This study demonstrated that SLs affected stem growth of cherry rootstocks by changing other endogenous hormone levels. These results provide a solid theoretical basis for using SLs to modulate plant height and achieve sweet cherry dwarfing and high-density cultivation.

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Section: Methodsmentioning

confidence: 99%

“…In addition, we counted the number, length and width of cells in stems of cherry rootstocks treated with different treatments. The preparation of paraffin sections followed the method of Gao et al (2021). The samples were observed, photographed, and analyzed using an Olympus BX61 microscope (Olympus, Tokyo, Japan).…”

Section: Paraffin Section Detectionmentioning

confidence: 99%

Strigolactones modulate stem length and diameter of cherry rootstocks through interaction with other hormone signaling pathways

Liu

Xu²,

Sun³

et al. 2023

Front. Plant Sci.

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“…Then put it in refrigerator at 4 ℃ overnight [24] . Dehydration: The peel tissues of JMM were taken out and dehydrated in 50%, 60%, 75%, 80%, 95% ethanol and anhydrous ethanol for 90 min respectively [25] .…”

Section: Microscopical Identi Cation Of Medicinal Materialsmentioning

confidence: 99%

Pharmacognostic Identification of the Green Peel of Juglans mandshurica Maxim. from Different Regions and Harvest Times

Jielin

Wang

Yan

et al. 2021

Preprint

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Background: The green peel of Juglans mandshurica Maxim. (QLY) can be used as medicine, also known as Qinglongyi[1]. The aim of this study was to identify QLY from the different regions and harvest times by pharmacognostic identification.Methods: In this study, the morphological character, microscopic character and juglone’s content of QLY from different regions and harvest times were compared by morphological identification, paraffin section and powder section, physico-chemical identification and High Performance Liquid Chromatography (HPLC).Results: Morphological identification results that the colour of outer surfaces of peels which harvested from Tangyuan county of Heilongjiang Province in July, August and September are respectively yellow brown, tan and black brown , and the color of the solution graded gradually from light brown yellow to brownish black by water-based test (WBT). The colour of outer surfaces of peels which harvested from Changbai county of Jilin Province in July, August and September are respectively yellowish white, brownish yellow and pale brown, and the color of the solution gradually changed from light brown to dark brown by WBT. As well as the colour of outer surfaces of peels which harvested from Qingyuan county of Liaoning Province in July, August and respectively September are Light yellowish green, light brownish green and brown green, and the color of the solution gradually changed from light green to dark brown by WBT. With the increase of growth period, the number of microscopic characteristics of different Qinglongyi increased. Through physico-chemical identification, it was found that the yield of juglone in QLY picked on September 21, 2016 was the highest, and the content of juglone, whose order was Heilongjiang > Jilin > Liaoning, was as high as 203.476 μg/g.Conclusions: Through comparison, it is concluded that Heilongjiang Province is one of the high-quality producing areas of QLY, and its best harvest time is in the middle and first ten-day period of September to harvest QLY without softening, yellowing and decay. This experiment provides a theoretical basis for the determination of the best harvest conditions in QLY and the establishment of identification standards for medicinal materials.

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Longitudinal section cell morphology of Chinese fir roots and the relationship between root structure and function

Zhang²,

Lin³

et al. 2023

Front. Ecol. Evol.

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IntroductionThe longitudinal section cell morphology of Chinese fir roots was studied to better understand the relationship between root structure and root function.MaterialsIn this study, the adjusted microwave paraffin section method and the selected two sample transparency methods were used to process the Chinese fir roots and combined with the laser scanning confocal microscopy (LSCM) technique, the morphology of Chinese fir roots longitudinal section can be clearly observed in a short time. At the same time, the observation effect of the longitudinal section cell morphology of the LSCM image of the thick section of the Chinese fir roots and the ordinary optical imaging of the thin section was analyzed and compared.Results and DiscussionThe results showed that: (1) There were apparent differences in the observation effect of cell morphology in longitudinal sections of Chinese fir roots obtained using various treatment methods. Under LSCM, the section with a thickness of 20 μm generated by the microwave paraffin section technique displayed complete cell morphology and clear structure in the root cap, meristem zone, and elongation zone. The overall imaging effect was good; the thickness was 0.42–1.01, 0.64–1.57, and 0.95–2.71 mm, respectively. The cell arrangement in maturation zone cells was more regular. (2) Compared to the ordinary optical imaging of thin sections, the thick sections of roots made by the microwave paraffin section method shortened the time to obtain high-quality sections to ensure the observation effect. Therefore, adopting the microwave paraffin cutting approach to produce thicker root sections under LSCM allows for rapid observation of the cell morphology in longitudinal sections of Chinese fir roots. The current study provides the efficient operation procedure for the microscopic observation technology of the longitudinal section of Chinese fir roots, which is not only beneficial to reveal the relationship between the root structure and function from the microscopic point of view but also provides a technical reference for the anatomical study of other organs and the observation of the longitudinal section cell morphology of plant roots with similar structural characteristics.

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Study on Paraffin Section Technology of Fruit Tumor Tissues in Cucumber (<i>Cucumis sativus</i> L.)

Cited by 3 publications

References 7 publications

Strigolactones modulate stem length and diameter of cherry rootstocks through interaction with other hormone signaling pathways

Strigolactones modulate stem length and diameter of cherry rootstocks through interaction with other hormone signaling pathways

Pharmacognostic Identification of the Green Peel of Juglans mandshurica Maxim. from Different Regions and Harvest Times

Longitudinal section cell morphology of Chinese fir roots and the relationship between root structure and function

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