Study on Optimal Extraction and Hypoglycemic Effect of Quercetin

Abstract

Quercetin was extracted from Portulaca oleracea L. through biphasic acid hydrolysis to investigate its potential as a suppressor of dipeptidyl peptidase IV (DPP-IV) and its hypoglycemic effect in type 2 diabetic mice. The extraction procedure was optimized utilizing the response surface method (RSM) in a single-factor experimental setting. An extraction efficiency of 0.675% was achieved using the following optimized parameters: 0.064 mol/L vitriol, 1 : 109.155 solid-liquid ratio, and 21.408 min ultrasonication. Overall, findings indicate the effectiveness of quercetin extraction. A mouse model for type 2 diabetes was established to receive oral treatment with various quercetin concentrations for 8 weeks. Fasting blood glucose (FBG) and the DPP-IV activity in the serum were significantly reduced. The weight and insulin levels of the mice in the quercetin group were raised compared to those in the model group ( P < 0.01). Quercetin dose-dependently inhibited postprandial blood glucose excursions, as demonstrated by the oral glucose tolerance test. These results confirmed that quercetin has hypoglycemic effects and considerably improves insulin sensitivity via DPP-IV targeting.

Network Pharmacology Analysis, Molecular Docking Integrated Experimental Verification Reveal the Mechanism of Gynostemma pentaphyllum in the Treatment of Type II Diabetes by Regulating the IRS1/PI3K/Akt Signaling Pathway

“…The results of molecular docking showed that nine active components had good binding ability with key protein INSR, including Ginkgolic acid (GA13:1), Ginkgolic acid (GA15:1), Gypenoside XXVII, Gypenoside XXVIII, Hesperetin, Nicotiflorin, Quercetin, Spinasterol and Gypenoside XVII. Recent studies show that hesperidin, quercetin and spinach sterol all have obvious therapeutic effects on diabetes [ 43 , 44 , 45 ], which further verifies the accuracy of screening active compounds by network pharmacology and molecular docking. In particular, we found that Gypenoside XVII could bind to six core proteins strongly, and the docking scores were high among the screened compounds.…”