Study on Increased Fructosyltranseferase (levansucrase) Production by Optimizing Culture Condition from Pediococcus acidilactici Strain in Shaking Batch Cultures

Youssef, Ghada A.; Youssef, Amany S.; Talha, Shimaa; El-Aassar, Samy A.

doi:10.9734/bpi/nvbs/v5/4723f

Cited by 2 publications

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“…In contrast, one day incubation time was the optimum for Enterococcus hirae (Yehia et al, 2015). Pediococcus acidilactici showed the highest levansucrase activity after 2 days of fermentation Youssef et al (2014).…”

Section: Optimization Of Fermentation Medium Parameters Using One Fac...mentioning

confidence: 83%

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Statistical Optimization of Fermentation Conditions by Plackett–Burman Methodology for a New Extracellular Cholesterol Oxidase-Producing Bacillus cytotoxicus Strain

2022

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Egyptian Journal of Botany http://ejbo.journals.ekb.eg/ 59 C HOLESTEROL oxidase (CHO) is an oxidoreductase flavoenzyme with significant biotechnological applications. Recently, microbial CHO has received a great attention for their wide applications in medicinal field. The objective of the present work details the screening of extracellular cholesterol oxidase (CHO) producing microorganisms. Cholesterol oxidase producing bacteria were isolated from various local soil samples and some dairy products. Twelve isolates were tested for cholesterol oxidase activity using the well diffusion agar method to assess their ability to decompose cholesterol. One isolate was finally selected based on the highest productivity of cholesterol oxidase and high degree of cholesterol decomposition. The selected isolate was characterized using morphological, physical and biochemical techniques. The isolated bacterium was identified as Bacillus cytotoxicus strain and confirmed molecularly using 16S rRNA gene sequencing and deposited in GenBank with accession number KY367576. Classical (one-variable-at-a-time) and statistical method (Plackett-Burman design) were used to optimize growth medium to enhance the production of CHO from Bacillus cytotoxicus. The statistical optimized medium exhibited 2.63-fold increase in enzyme activity (2.31U/mL) and 2.57-fold increase in cholesterol decomposition (88.9%) in comparison with the un-optimized basal medium. The results revealed that Bacillus cytotoxicus has great potential for cholesterol degradation and produced significant levels of extracellular CHO in an optimized medium.

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Section: Optimization Of Fermentation Medium Parameters Using One Fac...mentioning

confidence: 83%

“…(Lee et al, 1997) and Streptomyces rimosus (Srivastava et al, 2018). The pH may indirectly affect the dissociation degree of the medium components (Youssef et al, 2014).…”

Section: Optimization Of Fermentation Medium Parameters Using One Fac...mentioning

confidence: 99%

Statistical Optimization of Fermentation Conditions by Plackett–Burman Methodology for a New Extracellular Cholesterol Oxidase-Producing Bacillus cytotoxicus Strain

2022

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Remarkable improvement of levansucrase production from a newly isolated Aspergillus niger MK788296 strain using agro-industrial wastes through statistical optimization techniques

Ragab¹,

Elzairy²,

Mostafa³

et al. 2023

Egyptian Pharmaceutical Journal

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Background and objective Microbial levansucrase (LS) is a good source for the production of biologically active fructo-oligosaccharides and levan, which have diverse applications in pharmaceutical industries. Therefore, recent studies have focused on the enhancement of LS production through searching for potent microbial producers and optimization of the fermentation conditions. The present study aimed to use agro-industrial waste as a cost-effective carbon source for LS production and maximize the enzyme yield by optimization of the cultural conditions. Materials and methods A potent fungal producer of LS was isolated from an Egyptian soil sample that was collected from Giza Governorate at a depth of 5 cm and identified based on internal transcribed spacer identification and then submitted to the gene bank database. The production of LS by the isolated strain was optimized by evaluating the best fermentation state and agro-industrial waste to be used in the fermentation process. After that, further optimization of culture medium composition was established by two statistical designs: the Plackett–Burman design followed by central composite design. Results and conclusion The isolated strain was identified as Aspergillus niger MK788296. The first optimization approach declared that using the submerged fermentation technique and utilizing potato peels as the main carbon source led to a 2.4-fold increase in LS production. The statistical optimization resulted in a massive LS production (18870.3 U/ml) with a 59.4-fold increase in enzyme yield than the nonoptimized culture conditions.

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Study on Increased Fructosyltranseferase (levansucrase) Production by Optimizing Culture Condition from Pediococcus acidilactici Strain in Shaking Batch Cultures

Cited by 2 publications

References 0 publications

Statistical Optimization of Fermentation Conditions by Plackett–Burman Methodology for a New Extracellular Cholesterol Oxidase-Producing Bacillus cytotoxicus Strain

Statistical Optimization of Fermentation Conditions by Plackett–Burman Methodology for a New Extracellular Cholesterol Oxidase-Producing Bacillus cytotoxicus Strain

Remarkable improvement of levansucrase production from a newly isolated Aspergillus niger MK788296 strain using agro-industrial wastes through statistical optimization techniques

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