Study on Hypoglycemic Effect of the Drug Pair of Astragalus Radix and Dioscoreae Rhizoma in T2DM Rats by Network Pharmacology and Metabonomics

Abstract

Type 2 diabetes mellitus (T2DM) is a metabolic disease accompanied by a series of diseases such as diabetic nephropathy. The drug pair (HS) of Astragalus Radix (HQ) and Dioscoreae Rhizoma (SY) was designed by Dr. Shi Jinmo to improve the treatment of T2DM. However, the exact mechanism involved requires further clarification. In this work, 1H-NMR–based metabonomics and network pharmacology were adopted. Metabolic profiling indicated that the metabolic perturbation was reduced after HS treatment. The results found 21 biomarkers. According to the network pharmacology, we found that the regulation of T2DM was primarily associated with 18 active compounds in HS. These active compounds mainly had an effect on 135 targets. Subsequently, combining network pharmacology and metabonomics, we found four target proteins, which indicated that HS has potential hypoglycemic effects through regulating monoamine oxidases B (MAOB), acetyl-CoA carboxylase 1 (ACACA), carbonic anhydrase 2 (CA2), and catalase (CAT). In conclusion, the result showed that these four targets might be the most relevant targets for the treatment of T2DM with HS. This study clarified the mechanism of HS in the treatment of T2DM and also confirmed the feasibility of combining metabonomics and network pharmacology to study the mechanisms of traditional Chinese medicine (TCM). In the future, this approach may be a potentially powerful tool to discovery active components of traditional Chinese medicines and elucidate their mechanisms.

Identification of the Active Constituents and Significant Pathways of Cangfu Daotan Decoction for the Treatment of PCOS Based on Network Pharmacology

“…ese developments also lead to a new direction for the modernization and internationalization of TCM [54]. e combination of bioinformatics, computer technology, and experiments in the study of the pharmacodynamic components and potential targets of TCM broke through the deficiency of traditional studies is more rapid, flexible, and accurate and has been widely used in research on the network pharmacology of TCM [55].…”

Hypoglycemic effect of Taraxacum officinale root extract and its synergism with Radix Astragali extract

“…Abundant resources of dandelion can be obtained around the world (Yang & Zhang, 2020). Its flowers, leaves, and roots all have edible and medicinal value (Guo, Zhang, et al., 2019; Guo, Niu, et al., 2019). Dandelion contains amounts of polysaccharides, flavonoids, phenolic compounds, and terpenes (Diaz et al., 2018).…”

“…Dandelion contains amounts of polysaccharides, flavonoids, phenolic compounds, and terpenes (Diaz et al., 2018). These compounds decide that dandelion has biological functions of anticancer, anti‐inflammatory, antidiabetes, and antirheumatism (Gonzalez‐Castejon et al., 2012; Guo et al., 2019a; Guo, Niu, et al., 2019). Recent scientific researches showed that DRE could relieve the symptom of colitis in experimental mice and its abundant phenolic compounds showed excellent antioxidant property (Ding & Wen, 2018; Wirngo et al., 2016).…”

“…In vivo experiments, RAE could relieve the typical symptoms of T2DM rats; it participated in 14 metabolic pathways of rats and influenced 22 metabolites (Liu et al., 2017). Furthermore, RAE could control abnormal oxidative stress level in diabetic rats, thereby protecting the kidneys of rats from damage (Guo et al., 2019a, 2019b).…”

Combination of Astragalus membranaceous and Angelica sinensis Ameliorates Vascular Endothelial Cell Dysfunction by Inhibiting Oxidative Stress

“…However, because of the complicated composition of the ingredients, the mechanism of traditional Chinese formulations in alleviating diabetes mellitus remains ambiguous. Astragalus membranaceous , known as Huang Qi in China, is one of most prevalent traditional Chinese herbal medical compounds with known therapeutic effect against diabetes mellitus [ 12 – 14 ]. Astragalus membranaceous has also shown great antioxidative and anti-inflammatory effects [ 15 ].…”