Study on how mobile phase buffer composition effects the retention behavior of the system peak in non-suppressed ion chromatography

Nishimura, Masayuki; Hayashi, Morimasa; Yamamoto, Atsushi; Horikawa, Tatsuji; Hayakawa, Kazuichi; Miyazaki, Motoichi

doi:10.1016/0021-9673(95)00406-d

Cited by 10 publications

(3 citation statements)

References 11 publications

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“…Ion-exchange chromatography is a useful tool for the separation of not only simple ions but also macromolecules such as proteins and has been extensively used for the analysis and purification of ionic compounds. A large number of partition (alternatively chromatographic retention) data of ions have been accumulated for various ion-exchange resins differing in resin matrixes and/or chemical structures of ion-exchange sites as well as for various mobile phases since ion chromatography was introduced as an effective means for ionic analysis. − A number of studies are still devoted to enhancing separation selectivity and performance by varying resin structures and mobile-phase compositions − in order to exploit novel applications and to elucidate proper ion-exchange mechanisms.…”

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Interpretation of Ion-Exchange Chromatographic Retention Based on an Electrical Double-Layer Model

Okada

1998

Anal. Chem.

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An ion-exchange chromatographic model based on the Stern-Gouy-Chapman electrical double-layer theory is presented. We assume several equilibria occurring at the surface of an ion-exchange resin, such as the ion-pair formation of counterions with an ion-exchange site, the adsorption of ions, and the ion-pair formation of an adsorbed ions with their counterions. These equilibria are affected by the potential at the planes (the surface and the Stern layer potential) where the reactions occur. In addition, the nonselective accumulation of ions in the diffuse layer is also taken into account. Based on the developed model and derived equations, the nature of log k'-log X (X is the concentration of an eluent ion) plots is investigated for various sets of parameters and is compared with that of possible experimental results. Three extreme cases can be distinguished: (1) ion-pair-controlled, (2) adsorption-controlled, and (3) accumulation in the diffuse layer-controlled retention. Though log k'-log X plots, when they are studied over wide eluent concentration ranges (more than 2 orders), do not necessarily show precise linearity especially in the presence of eluent adsorption and extremely high eluent ion-pair formation, the linear regression analyses give negative unit slopes (within 10% deviations) for monovalent solute-eluent combinations. Since the deviation from linearity relation is mostly found in very low k' ranges (e.g., k' < 1), it is possible only in very limited cases to confirm nonlinearity experimentally. This may have mistakenly led to the idea that selectivity coefficients can be regarded as constants and thus the log k'-log X plots should be linear with the slope equal to (charge ratios) × (-1) in ion-exchange chromatographic experiments. The efficiency of the developed model is verified by its ability to predict experimental results, e.g., nonlinear log k'-log X plots, changes in separation factors with varying ion-exchange affinity of an eluent ion, adsorption isotherms at different salt concentration, etc.

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confidence: 99%

Interpretation of Ion-Exchange Chromatographic Retention Based on an Electrical Double-Layer Model

Okada

1998

Anal. Chem.

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“…The peaks for phosphate, chloride, bromide and nitrate and system peak are in Figure 1. The system peak often exists in unsuppressed anion-exchange analysis when organic acid is used as eluent [4,5]. When 20 #L deionized water sample is injected, the system peak also appear in the chromatogram.…”

Section: Test and Rearrangement Of Conductivity Detection Equationmentioning

confidence: 99%

Study of conductivity detection equation for unsuppressed anion-exchange chromatography

1999

Chromatographia

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“…A number of ion-exchange selectivity data (or alternatively chromatographic retention data) have been reported [41][42][43][44][45][46][47][48] , and have shown the variation of selectivity with the structures of ion-exchange sites as well as with mobile phase compositions; e.g. the larger ions are more preferably bound by the resin having bulky ionexchange sites of higher hydrophobicity, while the selectivity toward smaller ions is enhanced in aprotic solvents by hydrogen bond formation between active sites and ions.…”

Section: ·2·2 What Are Predicted By Modelsmentioning

confidence: 99%

Interpretation of Chromatographic Retention Based on Electrostatic Theories

Okada

1998

ANAL. SCI.

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Electrostatic potential is responsible for various phenomena, particularly for the behaviors of ionic species in solution and at various interfaces. In some modes of chromatography, electrostatic interaction often governs overall retention selectivity; ion-exchange chromatography is a typical example. Thus, though the understanding of electrostatic interaction is essential to interpret and predict chromatographic retention in some cases, sufficient attention has not been paid to its roles. There are some possible reasons for this situation: (1) direct estimation of the potential is usually difficult; (2) simple models not involving the contribution from electrostatic potential have given rather good explanations to experimental data. Some research groups have recently attempted to take electrostatic potential effects into consideration in various ways, and have successfully developed chromatographic models. These approaches are expected to facilitate understanding of chromatographic phenomena dominantly controlled by electrostatic interaction and to provide a view of more physical significance. In this review, the author mentions advantages of chromatographic models based on electrostatic theory over usual separated-phase models, and shows problems to be solved.

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Study on how mobile phase buffer composition effects the retention behavior of the system peak in non-suppressed ion chromatography

Cited by 10 publications

References 11 publications

Interpretation of Ion-Exchange Chromatographic Retention Based on an Electrical Double-Layer Model

Interpretation of Ion-Exchange Chromatographic Retention Based on an Electrical Double-Layer Model

Study of conductivity detection equation for unsuppressed anion-exchange chromatography

Interpretation of Chromatographic Retention Based on Electrostatic Theories

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