Study on hemostatic activities of the rhizome ofParis bashanensis

Abstract

Context: Paris bashanensis Wang et Tang (Liliaceae) is widely used in traditional Chinese medicine for the treatment of injuries, fractures and hemorrhage in Hubei and Sichuan Province. Objective: The n-BuOH extract of Paris bashanensis was investigated for hemostatic activity and chemical constituents in order to provide a basis for the application in folk use. Materials and methods:The n-BuOH extract of P. bashanensis was divided into three eluents (30, 50 and 70% EtOH) by macroporous adsorptive resin D101. The bleeding time of breaking tail hemostasis and clotting time of capillary and slide method in mice were used extensively to screen the hemostasis properties after repetitive administration of these three fractions (100 mg/kg, 50 mg/kg) for 5 days (total of 5 times, once per day). The chemical compounds were analyzed by HPLC-UV. Results: The inhibition rates in the bleeding time of 70, 50 and 30% n-BuOH ext. were 45, 32 and 21%, respectively. Using the slide method the decreasing rate of the clotting time of 70, 50 and 30% n-BuOH ext. were 71, 65 and 32% and in the experiment of capillary method, the inhibition rates were 43, 31 and 24%, respectively. A total of 70% n-BuOH ext. showed a high content of the pennogenin-type saponins by HPLC-UV. Discussion and conclusions: The 70% n-BuOH ext. of P. bashanensis was found to contain high levels of pennogenin saponins, which may lead to a higher hemostatic activity. Combined with the hemostatic test, P. bashanensis could be used as a resource of hemostatic drug.

Comparative study on hemostatic, cytotoxic and hemolytic activities of different species of Paris L.

“…They could reflect the whole process of blood coagulation since blood was eluted from the micrangium (Li et al, 2008). In our study, PY as the standard herbal medicine showed the hemostatic activity by reducing the values of bleeding time and clotting time, which was consisted with the reported (Wu et al, 1987;Wang et al, 1989). The other five species of Paris L. (PD, PF, PB, PM, and PP) could also shorten the values of bleeding time and clotting time in mice significantly, and PD, PB and PP showed more effective than the other groups.…”

Effect of Neurorepair for Motor Functional Recovery Enhanced by Total Saponins From Trillium tschonoskii Maxim. Treatment in a Rat Model of Focal Ischemia

“…It's worth noting that TSTT are mainly constituted by two kinds of steroidal saponins, pennogenin, and diosgenin saponins, which possess different biological and pharmacological properties (Hernandez-Vazquez et al, 2020;Ahmad et al, 2021). Specifically, pennogenin saponins have antiinflammatory, anti-tumor and anti-hemorrhagic activity (Wang et al, 2013). For example, Paris saponin VII showed robust effect of anti-inflammation in LPS-stimulated RAW264.7 cells by reducing the levels of pro-inflammatory cytokines and enzymes, simultaneously inhibited the inflammation response to ear edema and granuloma formation in mice (Zhang C. et al, 2019).…”

Isolation of a Novel Metalloproteinase from Agkistrodon Venom and Its Antithrombotic Activity Analysis

“…The average time of each observation was 20 s. The collected blood samples were also spotted on glass slides and clotting times determined. Clotting was achieved when the fibrin mesh appeared and could be picked by glass dissecting needle [47,48].…”