Study on Growth Influencing Factors and Desulfurization Performance of Sulfate Reducing Bacteria Based on the Response Surface Methodology

Abstract

Sulfate reducing bacteria (SRB) can simultaneously and efficiently remove SO 4 2− and heavy metal ions from acid mine drainage (AMD). Environmental factors have a great influence on AMD treated by SRB metabolic reducing sulfate. Providing a suitable growth environment can improve the effect of SRB on AMD. In this paper, the wet soil around the tailings reservoir was used as seed mud to enrich SRB. Based on the single factor experiment method and the response surface methodology (RSM), the effects of temperature, environmental pH value, S 2− concentration, and COD/SO 4 2− on the growth of SRB were analyzed. The effects of environmental factors such as temperature and pH on the desulfurization performance of SRB were investigated. The results showed that the growth curve of SRB was "S" type. SRB was in the logarithmic phase when cultured for 14−86 h, with high activity and vigorous growth metabolism. When the temperature is 32∼35 °C, the activity of SRB is the highest. With the gradual increase of the S 2− concentration in the culture system, SRB activity will be inhibited and even lead to SRB cell death. The environmental pH value that SRB can tolerate is 5∼8, and when the environmental pH value is 7∼8, the SRB activity is the strongest. The chemical oxygen demand (COD)/SO 4 2− that is most suitable for SRB growth is 2. The optimal growth conditions of SRB obtained from RSM were as follows: culture temperature at 34.74 °C, initial pH being 8.00, and initial COD/ SO 4 2− being 1.98. Under these conditions, the OD 600 value was 1.45, the pH value was 9.37, the oxidation reduction potential (ORP) value was −399 mV, and the removal percentage of SO 4 2− was 88.74%. The results of RSM showed that the effects of culture temperature, environmental pH, and COD/SO 4 2− on the desulfurization performance of SRB were extremely significant. The order of affecting the removal of SO 4 2− by SRB was environmental pH > temperature > COD/SO 4 2− .

Immobilization Behavior and Mechanism of Cd2+ by Sulfate-Reducing Bacteria in Anoxic Environments

“…Under each temperature condition, the pH of the solution showed a gradual increase with the growth of the bacterial strains, with the most significant increase observed at 30 • C (Figure 3a). This is mainly because SRB exhibit high growth activity and a large population size at this temperature, resulting in higher metabolic alkalinity [26]. Figure 3c indicates that the OD600 value of the solution was lowest at 20 • C and highest at 30 • C, further suggesting optimal SRB growth conditions at this temperature, which is consistent with the findings reported by Sokolova [27].…”

“…Under each temperature condition, the pH of the solution showed a gradual increase with the growth of the bacterial strains, with the most significant increase observed at 30 °C (Figure 3a). This is mainly because SRB exhibit high growth activity and a large population size at this temperature, resulting in higher metabolic alkalinity [26]. Microbial cell growth involves a series of enzyme−catalyzed reactions, and excessively high or low temperatures can inhibit the activity of certain enzymes in these cells.…”

Physicochemical Variation of Water, Diversity, and Bacterial Community Structure in Post-coal Mining Ponds of Different Ages in Samarinda, East Kalimantan, Indonesia

“…This showed that a high density of bacteria caused a lower concentration of sulfur, temperature, conductivity, and DO. The results showed that environmental factors affected the activity of enzymes in bacteria, thereby influencing the efficiency of SOB in treating sulfur (Dong et al, 2023). The results of the Venn analysis obtained in Figure 4 showed that coal post-mining ponds samples with age of > 20 years had more OTUs when compared to those aged < 5 years.…”

Sustainable reduction of sulfate contained in gypsum waste: perspectives and applications for agroforestry waste and sanitary sewage

“…The process can occur with or without agitation and aeration through diffusion (shaker incubator with orbital shaking and heating). Dong et al (2023) used Erlenmeyer flasks to monitor the growth of SRB. These bacteria exhibited an "S-"type growth curve, and their logarithmic growth phase was quantified throughout 14-86 h, demonstrating high activity and robust growth metabolism.…”