Study on genetic variability in MHC-DRB1 second exon in Makuie sheep breed population

Abstract

In the present study polymorphism of the exon 2 of MHC (Major Histocompatibility Complex) gene in Makuie sheep breed was studied. Genomic DNA from blood samples of 90 sheep was extracted and a 279 bp MHC exon 2 fragment was amplified using polymerase chain reaction (PCR). PCR products were subjected to enzymatic digestion using RsaI endonuclease. Digested PCR products were electrophoresed on 2% agarose gel. The results showed the existence of 10 alleles: A, B, E, F, I, M, O, P, Q and V for the exon 2 of the MHC gene, with the frequencies of 0.4756, 0.0976, 0.0183, 0.0366, 0.0549, 0.0122, 0.1098, 0.0915, 0.0854 and 0.0183, respectively. Eighteen genotypes: AA, AB, AE, FF, AM, BO, EO, IO, OM, AP, BP, OP, PP, AQ, OQ, PQ, QQ and AV with the frequencies of 0.317, 0.1585, 0.0121, 0.0365, 0.0121, 0.0243, 0.0243, 0.1097, 0.0121, 0.0487, 0.0121, 0.0365, 0.0365, 0.0487, 0.0121, 0.0121, 0.0487 and 0.0365, respectively were identified in the population under study. Effective number of alleles and heterozygosity for the examined region were 3.7231 and 0.7314, respectively. Chi-square test showed that the examined sheep population was not in Hardy-Weinberg equilibrium in the examined region. <br><br><font color="red"><b> This article has been corrected. Link to the correction <u><a href="http://dx.doi.org/10.2298/GENRSR1602800A">10.2298/GENRSR1602800A</a><u></b></font>

Fecal egg counts for gastrointestinal nematodes are associated with a polymorphism in the MHC-DRB1 gene in the Iranian Ghezel sheep breed

“…A particular focus of the study is the abomasal GIN Haemonchus contortus and was justified by this parasite’s ability to produce large numbers of eggs, resulting in extensive pasture contamination; the blood-sucking nature of this nematode, which can causes life-threatening levels of anemia; and the associated potential for very significant reductions in lamb performance and survival ( Gatongi et al, 1998 ; Waller et al, 2004 ). In Iran, Ashrafi et al (2014) demonstrated polymorphism in exon 2 of MHC gene OLA-DRB1 in the Makui sheep breed of Northwestern Iran. In this study, our aim was thus to explore possible associations between nematode resistance and polymorphism of DRB1.…”

Associations of polymorphisms in the prolactin receptor gene with growth trait in Japanese quail (Coturnix coturnix japonica)

“…Different methods have been used to study genetic variation in the PRLR gene in various birds and animals. Among different methods, PCR-SSCP analysis has been found a valuable technique in identifying genetic variation of the PRLR gene in farm animals (KONNAI et al, 2003;GRUSZYNSKA et al, 2005, ASHRAFI et al, 2014.…”

Association between the Ovine MHC-DRB1 Gene and its Resistance to Gastrointestinal Parasites in Deccani Sheep Raised in Hot Semi-arid Ecosystem of India

“…Many researchers link the genes in the sheep major histocompatibility complex (MHC) with the ability of sheep to resist infection by GIN as measured by feacal egg count (FEC) and revealed polymorphisms in exon 2 and adjoining intron 2 of the expressed gene DRB1. The DRB1 exon 2 encodes 1 domain, which constitutes part of the protein binding receptor (PBR) or T-cell receptors of the DR molecules and is likely to be related to functionality such as disease resistance/susceptibility (Paterson et al, 1998;Charon et al, 2002;Ashrafi et al, 2014 andValilou et al, 2015). The DR genes are highly polymorphic in nature and proteins encoded by these genes are expressed in elevated concentrations, specifically on the cell membranes of macrophages and B cells (Outteridge et al, 1996).…”