Study on gelatin-containing artificial skin: I. Preparation and characteristics of novel gelatin-alginate sponge

Choi, Yang‐Kyu; Hong, Sung Ran; Lee, Young Moo; Song, Kang Won; Park, Moon Hyang; Nam, Young Jin

doi:10.1016/s0142-9612(98)00180-x

Cited by 372 publications

(187 citation statements)

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“…A nonexhaustive overview of the most recent publications, subdivided by application, for either collagen or gelatine alone or in a combination of other biopolymers is summarized in Table 2 which clearly indicates that gelatine has a wider-application range within the field of both soft and hard-tissue engineering. (Spira et al, 2004) skin replacement artificial skin dermis (Harriger et al, 1998) skin tissue engineering Tangsadthakun et al, 2006) artificial skin (Choi et al, 1999;Lee et al, 2003) soft tissue adhesives (McDermott et al, 2004) Surgery hemostatic agent (Cameron, 1978;Browder & Litwin, 1986) plasma expander suture wound dressing and repair (Rao, 1995) skin replacement (artificial skin) nerve repair and conduits blood vessel prostheses (Auger et al, 1998;McGuigan et al, 2006, Amiel et al, 2006 small intestine ( Chiu et al, 2009) liver -chitosan/gelatine scaffold (Jiankang et al, 2007) wound dressing (Tucci & Ricotti, 2001) nerve regenerationchitosan/gelatin scaffolds (Chiono et al, 2008) blod vesels ( Mironov et al, 2005) Orthopaedic born, tendon and ligament repair cartilage reconstruction -collagen (Stone, 1997), composite of collagen type II/chondroitin/hyaluronan (Jančar et al, 2007) articular cartilagecollagen/chitosan (Yan et al, 2010) bone substitutegelatine/hydroxyapatite (Chang et al, 2007) hard tissue regenerationgelatine/hydroxyapatite ( Kim et al, 2005) cartilage (Lien et al, 2010) cartilage defects regenerationchitosan/ gelatine (Guo et al, 2006), ceramic/ gelatine bone substitutegelatine/tricalcium phosphate (Yao et al, 2005) Ophthalmology corneal graft (Lass et al, 1986) vitreous implants artificial tears (Kaufman et al, 1994) tape and retinal reattachment contact lenses eye disease treatment (http.....) ocu...…”

Section: Collagen Vs Gelatine As Biomaterialsmentioning

confidence: 99%

“…Collagen has also been used to improve cells´ interactions with electrospun nanofibers of poly (hydroxyl acids), such as poly(lactic acid), poly(glycolic acid), poly( -caprolactone), and their copolymers (Pachence et al, 2007). Novel gelatine/alginate sponge serving as an drug carrier for silver sulfadiazine and gentamicin sulphate, used for wound healing (Choi et al, 1999). Alginate is known as a hydrophilic and biocompatible polysaccharide, and is commonly used in medical applications, such as wound dressing, scaffolds for hepatocyte culture, and surgical and dental materials.…”

Section: Combination With Other Biopolymersmentioning

confidence: 99%

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Collagen- vs. Gelatine-Based Biomaterials and Their Biocompatibility: Review and Perspectives

Gorgieva¹,

Kokol²

2011

Biomaterials Applications for Nanomedicine

242

250

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Section: Collagen Vs Gelatine As Biomaterialsmentioning

confidence: 99%

Section: Combination With Other Biopolymersmentioning

confidence: 99%

Collagen- vs. Gelatine-Based Biomaterials and Their Biocompatibility: Review and Perspectives

Gorgieva¹,

Kokol²

2011

Biomaterials Applications for Nanomedicine

242

250

View full text Add to dashboard Cite

“…There are generally two types of gelatin: Type A and Type B. Gelatin Type A is extracted and processed by acidic pretreatment of collagen, whereas gelatin Type B is obtained by alkaline pretreatment. The alkaline pretreatment converts glutamine and asparagine residues into glutamic and aspartic acids, respectively, which leads to higher carboxylic acid content for gelatin Type B than for gelatin Type A. Gelatin has several potential advantages over other natural proteins, such as its biological origin, biodegradability and commercial availability at low cost [24][25][26][27][28][29][30][31][32][33].…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

et al. 2008

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Electrospinning using natural proteins or synthetic polymers is a promising technique for the fabrication of fibrous scaffolds for various tissue engineering applications. However, one limitation of scaffolds electrospun from natural proteins is the need to cross-link with glutaraldehyde for stability, which has been postulated to lead to many complications in vivo including graft failure. In this study, we determined the characteristics of hybrid scaffolds composed of natural proteins including collagen and elastin, as well as gelatin, and the synthetic polymer poly(ε-caprolactone) (PCL), so to avoid chemical cross-linking. Fiber size increased proportionally with increasing protein and polymer concentrations, whereas pore size decreased. Electrospun gelatin/PCL scaffolds showed a higher tensile strength when compared to collagen/elastin/PCL constructs. To determine the effects of pore size on cell attachment and migration, both hybrid scaffolds were seeded with adipose-derived stem cells. Scanning electron microscopy and nuclei staining of cell-seeded scaffolds demonstrated complete cell attachment to the surfaces of both hybrid scaffolds, although cell migration into the scaffold was predominantly seen in the gelatin/PCL hybrid. The combination of natural proteins and synthetic polymers to create electrospun fibrous structures resulted in scaffolds with favorable mechanical and biological properties.

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“…Alginate-gelatin (Alg-Gel) blends have been reported as potential drug delivery carriers 8,9 , enzyme immobilisation beads 5 22 , wound dressing fibres 23 , and sponges for tissue matrices 24 . Among the studies related to bioprinting, Yan and co-workers [25][26][27] have attempted to print 3D scaffolds from alginate-gelatin blends.…”

mentioning

confidence: 99%

Bio-ink properties and printability for extrusion printing living cells

et al. 2013

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Additive biofabrication (3D bioprinting) makes it possible to create scaffolds with precise geometries, control over pore interconnectivity and architectures that are not possible with conventional techniques. Inclusion of cells within the ink to form a "bio-ink" presents the potential to print 3D structures that can be implanted into damaged/diseased tissue to promote highly controlled cell-based regeneration and repair. The properties of an 'ink' are defined by its formulation and critically influence the delivery and integrity of structure formed. Importantly, the ink properties need to conform to biological requirements necessary for the cell system that they are intended to support and it is often challenging to find conditions for printing that facilitate this critical aspect of tissue bioengineering. In this study, alginate (Alg) was selected as the major component of the 'bio-ink' formulations for extrusion printing of cells. The rheological properties of alginate-gelatin (AlgGel) blends were compared with pre-crosslinked alginate and alginate solution to establish their printability whilst maintaining their ability to support optimal cell growth. Pre-crosslinked alginate on its own was liquidlike during printing. However, by controlling the temperature, Alg-Gel formulations had higher viscosity, storage modulus and consistency which facilitated higher print resolution/precision. Compression and indentation testing were used to examine the mechanical properties of alginate compared to Alg-Gel. Both types of gels yielded similar results with modulus increasing with alginate concentration. Decay in mechanical properties over time suggests that Alg-Gel slowly degrades in cell culture media with more than 60% decrease in initial modulus over 7 days. The viability of primary myoblasts delivered as a myoblast/Alg-Gel bio-ink was not affected by the printing process, indicating that the Alg-Gel matrix provides a potential means to print 3D constructs that may find application in myoregenerative applications. control over pore interconnectivity and architectures that are not possible with conventional techniques. Inclusion of cells within the ink to form a "bio-ink" presents the potential to print 3D structures that can be implanted into damaged/diseased tissue to promote highly controlled cell-based regeneration and repair. The properties of an 'ink' are defined by its formulation and critically influence the delivery and integrity of structure formed. Importantly, the ink properties need to conform to biological requirements 10 necessary for the cell system that they are intended to support and it is often challenging to find conditions for printing that facilitate this critical aspect of tissue bioengineering. In this study, alginate (Alg) was selected as the major component of the 'bio-ink' formulations for extrusion printing of cells. The rheological properties of alginate-gelatin (Alg-Gel) blends were compared with pre-crosslinked alginate and alginate solution to establish their printability whil...

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Study on gelatin-containing artificial skin: I. Preparation and characteristics of novel gelatin-alginate sponge

Cited by 372 publications

References 0 publications

Collagen- vs. Gelatine-Based Biomaterials and Their Biocompatibility: Review and Perspectives

Collagen- vs. Gelatine-Based Biomaterials and Their Biocompatibility: Review and Perspectives

Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

Bio-ink properties and printability for extrusion printing living cells

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