Study on Extraction, Physicochemical Properties, and Bacterio-Static Activity of Polysaccharides from Phellinus linteus

Abstract

We optimized an ultrasound-assisted extraction process of Phellinus linteus mycelium polysaccharides (PLPs) and studied their monosaccharide composition and bacteriostatic properties. Based on a single-factor experiment, a three-factor, three-level Box–Behnken design was used to optimize the ultrasound-assisted extraction process of PLP, using the yield of PLP as the index. The chemical composition and monosaccharide composition of PLP were determined by chemical analysis and HPLC analysis, respectively. Microscopic morphological analysis of the surface of PLP was performed via swept-surface electron microscopy. The bacteriostatic properties of PLP were determined using the spectrophotometric turbidimetric method. The results showed that the best extraction process of PLP with ultrasonic assistance achieved a result of 1:42 g/mL. In this method, the ultrasonic temperature was 60 °C, ultrasonic extraction was performed for 20 min, and the yield of PLP was 12.98%. The monosaccharide composition of PLP mainly contains glucose (Glc), mannose (Man), galactose (Gal), and glucuronic acid (GlcA). The intracellular polysaccharide of Phellinus igniarius Mycelia (PIP) is an irregular spherical accumulation, the surface is rough and not smooth, and the extracellular polysaccharide (PEP) is a crumbly accumulation. PIP has a stronger inhibitory ability for S. aureus and E. coli and a slightly weaker inhibitory effect for B. subtilis; the inhibitory effect of PEP on S. aureus, E. coli, and B. subtilis is slightly inferior to that of PIP.

Synthesis of Pseudostellaria heterophyllapolysaccharide‐gold nanocomposites and their antitumor effect through immunomodulation

“…The purified PHP-1 was homogeneous and its molecular weight was determined by high-performance gel permeation chromatography (HPGPC) analysis (Li et al, 2022;Zhang et al, 2021) to be 60.99 kD. And the monosaccharide composition of PHP-1 was determined by high-performance liquid chromatography (HPLC) analysis (Eom et al, 2021;Gao et al, 2023) as ribose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose at a molar percentage of 0.9: 2.5: 0.5: 93.1: 1: 0.9 (Pu et al, 2022). The DMEM (Dulbecco's modified Eagles medium) and FBS (fetal bovine serum) were purchased from Gibco (NY, USA).…”

A review on the cultivation, bioactive compounds, health-promoting factors and clinical trials of medicinal mushrooms Taiwanofungus camphoratus, Inonotus obliquus and Tropicoporus linteus

“…A polysaccharide composed of glucose, galactose, mannose, fucose, and xylose in a molar ratio of 4.36: 2.34: 2.09: 0.88: 0.42 showed prebiotic potentials by increasing amounts of beneficial bacteria, such as Bacteroides , Prevotella , and Butyricimonas , and reducing pathogenic bacteria including Escherichia , Shigella , Morganella , and Intestinimonas [ 129 ]. Besides that, a polysaccharide with a monosaccharide composition of fucose, rhamnose, arabinose, glucuronic acid, galactose, glucose, and xylose (1.4:0.5:0.9:1.6:4.7:84.8:6.0) exerted bacterio-static activities against bacteria Staphylococcus aureus , Escherichia coli , and Bacillus subtilis , demonstrating the potential of T. linteus polysaccharides for clinical applications [ 130 ].…”