Study on Enterotoxigenic Escherichia coli Producing Extended Spectrum Beta Lactamase (ESBL) from Chicken Meat and its Products

Abstract

This study aimed to identify the prevalence of enterotoxigenic E. coli producing ESBL in some chicken meat and chicken meat products. A total of 300 samples, including 120 chicken meat samples, 180 nuggets, frankfurter and luncheon (60 each) were taken from different supermarkets of different sanitation levels. These samples were subjected to bacteriological examination. The results showed that the prevalence of E. coli in chicken meat, nuggets, frankfurter and luncheon was 36.7, 16.6, 20 and 26.7%, respectively. In addition, the mean values of Aerobic plate count in chicken meat, nuggets, frankfurter and luncheon were 2.05×10 5 ±0.43×10 4 , 3.4×10 3 ±0.22×10 3 , 4.7×10 3 ±0.11×10 3 and 6.5×10 3 ±0.15×10 3 cfu/g; and the mean values of Coliform count were 2.4×10 3 ±0.11×10 3 , 6.3×10 2 ±0.36×10 2 , 8.24×10 2 ±0.38×10 2 and 8.5×10 2 ± 0.34×10 2 cfu/g, respectively. E. coli isolates were serogrouped into O86:k61, O119:k69, O55:k59, O125:k70, O25:k11, O128:k67, O78:k80 and untyped serogroups. Antimicrobial susceptibility testing of most isolates revealed the presence of multidrug-resistant strains. In addition, virulence genes (Sta, Lt and Stx2 genes) were detected in 2, 1 and 4 isolates; and antibiotic resistance genes (bla TEM, Bla CTX-M and AmpC genes) were detected in 100% of isolates indicating high resistance to beta-lactamase antibiotics.

Effects of hempseed ( Cannabis sativa L. ) in diet on growth, gut health, and immunity in broilers

“…The resultant stained smears were then subjected to a microscopic examination using a Nikon YS 100 microscope to count WBCs using Neubauer chamber (Hassan et al. 2022 , 2023 ). Total leucocyte count was calculated using the following formula…”