Study on embryonic development and early growth of triploid and gynogenetic diploid left-eyed flounder,Paralichthys olivaceus (T. et S.)

You, Feng; Liu, Jing; Wang, Xin-cheng; Xu, Yide; Rui-dong, Huang; Zhang, Peijun

doi:10.1007/bf02863039

Cited by 35 publications

(20 citation statements)

References 11 publications

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“…Similar results were also reported in other flatfishes (Yamamoto 1999; Morgan et al 2006). The Japanese flounder showed a range of 10-30% survival to metamorphosis (You et al 2001). Peruzzi and Chatain (2000) demonstrated that very few European sea bass survived post-hatch after pressure or temperature shockinduced gynogenesis.…”

Section: Discussionmentioning

confidence: 98%

“…In flow cytometry analysis, 2 h after hatching, 30 larvae per group were selected randomly and sampled according to You et al (2001). Diploid larvae were used as a diploid standard for the calibration of the cytometer.…”

Section: Assessment Of Ploidy Level and Larval Survivalmentioning

confidence: 99%

“…Left-eyed flounder P. olivaceus sperm was chosen as heterologous sperm because P. olivaceus is a marine teleost that spermiates well in captivity (You et al 2001) and when used to fertilize turbot eggs should not produce a viable, hybridized offspring.…”

Section: Introductionmentioning

confidence: 99%

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Induction of diploid gynogenesis in turbot Scophthalmus maximus with left-eyed flounder Paralichthys olivaceus sperm

You

Sun

et al. 2008

Aquacult Int

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Turbot Scophthalmus maximus exhibits sexually dimorphic growth, with females growing faster and reaching larger adult sizes than males. Thus, development of techniques for preferentially producing females is necessary to optimize production of these species. In this paper, gynogenetic diploids of turbot were induced by activating egg development with ultraviolet (UV)-irradiated left-eyed flounder Paralichthys olivaceus sperm combined with cold shock to prevent extrusion of the second polar body. The results of UV irradiation experiments showed that survival, motility, and duration of activity of P. olivaceus sperm generally decreased with increase in UV dose. The typical Hertwig's effect was observed after fertilized turbot eggs with UV-irradiated P. olivaceus sperm and the optimal UV dose for gynogenetic haploid production was 36,000 erg mm -2 . At 15°C, appropriate timing of cold shock for retention of the second polar body in turbot eggs was at 6 min after fertilization. Results of different combinations of two shock temperatures (1 or 3°C) and four shock durations (15, 25, 35 or 45 min) at 6 min after fertilization demonstrated that shock of 25 min at 1°C gave the highest production of diploid gynogens (39.58% relative to its diploid control). The results of this study reveal that the use of UVirradiated P. olivaceus sperm for activation of turbot eggs and cold shock for polar body retention is an effective method to produce gynogenetic offspring.

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Section: Discussionmentioning

confidence: 98%

Section: Assessment Of Ploidy Level and Larval Survivalmentioning

confidence: 99%

See 1 more Smart Citation

Induction of diploid gynogenesis in turbot Scophthalmus maximus with left-eyed flounder Paralichthys olivaceus sperm

You

Sun

et al. 2008

Aquacult Int

Self Cite

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show abstract

“…The Japanese flounder showed a range of 10-30% survival to metamorphosis (You et al 2001). Peruzzi and Chatain (2000) demonstrated that very few European sea bass survived post-hatch after pressure or temperature shock-induced gynogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…In flow-cytometry analysis, just after hatching, 30 larvae per group were selected randomly and sampled according to You et al (2001). Diploid larvae were used as a diploid standard for the calibration of the cytometer.…”

Section: Assessment Of Ploidy Levelmentioning

confidence: 99%

Effects of ultra-violet irradiation on sperm motility and diploid gynogenesis induction in large yellow croaker (Pseudosciaena crocea) undergoing cold shock

You

Yan

et al. 2007

Aquacult Int

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Large yellow croaker, Pseudosciaena crocea, exhibit sexually dimorphic growth, with females growing faster and reaching larger adult sizes than males. Thus, development of techniques for preferentially producing females is necessary to optimize production of these species. We have established a protocol to produce all-female croaker P. crocea through induction of meiotic gynogenesis with homologous sperm. The first set of experiments investigated the ultra-violet (UV) irradiation on sperm motility and duration of sperm activity to determine the optimal UV dosage for genetic inactivation of sperm, yet retaining adequate motility for activation of eggs. Milt from several males was diluted 1:100 with Ringer's solution and UV irradiated with doses ranging from 0-150 J cm À2 . The results indicated that motility and duration of activity generally decreased with increased UV doses. At UV doses greater than 105 J cm À2 , after fertilization, motility was <10% and fertilization rates were significantly lower. Highest hatching rate was obtained at 75 J cm À2 . A second set of experiments was carried out to determine appropriate conditions of cold shock for retention of the 2nd polar body in P. crocea eggs after fertilization with UV-inactivated sperm by altering the timing, temperature and duration of shock. At 208C, shock applied at 3 min after fertilization resulted in higher survival rate of larvae at 6 h after hatching. Results of different combinations of three shock temperatures (28C, 38C or 48C) and five shock durations (4 min, 8 min, 12 min, 16 min or 20 min) at 3 min after fertilization demonstrated that shocks of 12 min gave highest production of diploid gynogens. Statistical analysis revealed that maximum production of diploid gynogens (44.55 ± 2.99%) were obtained at 38C. The results of this study indicate that the use of UV-irradiated homologous sperm for activation of P. crocea eggs and cold shock for polar body retention is an effective method for producing gynogenetic offspring.

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Analyses of mRNA‐seq and miRNA‐seq of the brain reveal the sex differences of gene expression and regulation before and during gonadal differentiation in 17β‐estradiol or 17α‐methyltestosterone‐induced olive flounder (Paralichthys olivaceus)

Zou

Fan

et al. 2019

Molecular Reproduction Devel

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Olive flounder (Paralichthys olivaceus) is a commercially important flatfish species cultured in East Asia. Female flounders generally grow more rapidly than males, therefore control of the sex ratio seems to be a proposed way to increase production.However, the sex determination gene and sex determination mechanism have yet been elucidated. The brain is an important organ that is involved in gonadal development. To explore the sex differences of gene expression in the brain before and during the flounder gonadal differentiation, we used messenger RNA (mRNA)-seq technology to investigate transcriptomes of male and female brains. Between female and male brains, 103 genes were differentially expressed before ovarian differentiation, 16 genes were differentially expressed before testicular differentiation, and 64 genes were differentially expressed during gonadal differentiation. According to annotation and Kyoto Encyclopedia of Genes and Genomes information, the differentially expressed genes (DEGs) were involved in circadian rhythm, circadian rhythm-fly, circadian entrainment, dopaminergic synapse, calcium signaling, glutamatergic synapse, taste transduction, herpes simplex infection, long-term depression, retrograde endocannabinoid signaling, and the synaptic vesicle cycle pathways.MicroRNA (miRNA)-seq was performed during the gonadal differentiation and the target genes of miRNAs were predicted. Integrated analysis of mRNA-seq and miRNA-seq showed that 29 of the 64 DEGs were regulated by the differentially expressed miRNAs during the gonadal differentiation. Our study provides a basis for further studies of brain sex differentiation and the molecular mechanism of sex determination in olive flounder.K E Y W O R D S brain, gonadal differentiation, miRNA-seq, mRNA-seq, olive flounder (Paralichthys olivaceus)

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Study on embryonic development and early growth of triploid and gynogenetic diploid left-eyed flounder,Paralichthys olivaceus (T. et S.)

Cited by 35 publications

References 11 publications

Induction of diploid gynogenesis in turbot Scophthalmus maximus with left-eyed flounder Paralichthys olivaceus sperm

Induction of diploid gynogenesis in turbot Scophthalmus maximus with left-eyed flounder Paralichthys olivaceus sperm

Effects of ultra-violet irradiation on sperm motility and diploid gynogenesis induction in large yellow croaker (Pseudosciaena crocea) undergoing cold shock

Analyses of mRNA‐seq and miRNA‐seq of the brain reveal the sex differences of gene expression and regulation before and during gonadal differentiation in 17β‐estradiol or 17α‐methyltestosterone‐induced olive flounder (Paralichthys olivaceus)

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