Study on effect of peptide-conjugated near-infrared fluorescent quantum dots on the clone formation, proliferation, apoptosis, and tumorigenicity ability of human buccal squamous cell carcinoma cell line BcaCD885

Sun, de ping

doi:10.2147/ijn.s10778

Cited by 20 publications

(15 citation statements)

References 22 publications

(40 reference statements)

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“…Previous studies have demonstrated that QDs at the experimental concentration are not cytotoxic and do not affect the growth and differentiation of the live cells (3,4,23). Our previous studies have also shown that labeling of cancer cells with NIRF-QDs do not affect their growth, proliferation, apoptosis, invasion, metastasis or the ability to form tumors (10,(24)(25)(26). Clinically, optical examination is a safe, simple and economical approach (18,27).…”

Section: Discussionmentioning

confidence: 90%

“…Previous studies have suggested that biologically functionalized QDs have excellent biological compatible and are water soluble (3,4,23). In addition, they do not cause side effects on live subjects and do not affect cellular growth, differentiation and function within the range of experimental doses (3,4,10,(23)(24)(25)(26). However, the core of QDs is typically made up of heavy metal elements, e.g., plutonium, cadmium and mercury.…”

Section: Discussionmentioning

confidence: 99%

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In vivo and in situ imaging of head and neck squamous cell carcinoma using near-infrared fluorescent quantum dot probes conjugated with epidermal growth factor receptor monoclonal antibodies in mice

et al. 2012

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Abstract. In this study, we applied near-infrared fluorescent quantum dots (NIRF-QDs) for non-invasive in vivo and in situ imaging of head and neck squamous cell carcinoma (HNSCC). The U14 squamous cancer cell line with high expression of epidermal growth factor receptor (EGFR) was implanted subcutaneously into the head and neck regions of nude mice to establish HNSCC models. NIRF-QDs with an emission wavelength of 800 nm (NIRF-QD800) were conjugated with EGFR monoclonal antibodies to develop the QD800-EGFR Ab probe. In vivo and in vitro studies demonstrated that the QD800-EGFR Ab probe can specifically bind EGFR expressed on U14 cells. U14 squamous cell carcinoma in the head and neck can be clearly visualized by in vivo imaging after intravenous injection of QD800-EGFR Ab probes. The results suggested that in situ imaging using NIRF-QD-EGFR Ab probes has unique advantages and prospects for the investigation of tumor development, early diagnosis and personalized therapy of HNSCC. IntroductionNon-invasive in vivo and in situ imaging of tumor cells plays an important role in studying the occurrence and development of cancers, making early diagnosis and conducting personalized therapies. This has been a difficult area due to the lack of highly sensitive imaging substances and specific marker for each particular type of tumor. Quantum dots (QDs) were developed recently and have shown great prospect in the noninvasive imaging of tumors (1,2).QD is a type of nanocrystal composed of elements belonging to Ⅱ-Ⅵ or Ⅲ-Ⅴ groups with a diameter of 2-10 nm. In comparison to the conventional organic fluorescent dyes and fluorescent proteins, QDs have unique optical properties, e.g., broad and continuous distribution of the excitation spectrum, narrow and symmetric emission spectrum, strong fluorescence and high photochemical stability. In addition, QDs are less prone to photobleaching and the spectrum of any points from ultraviolet to the near infrared can be obtained by changing the size and composition of QDs (3,4). These optical properties are not owned by all the current fluorescent probes. Currently, QDs have been applied in the imaging of biological macromolecules and cells both in vitro and in vivo (5-7). Non-invasive in vivo imaging has been used for the investigation of tumor development (8-10), early diagnosis of cancer (10), the transportation of drugs in vivo (11), and monitoring of therapeutic responses in vivo (11)(12)(13). These studies have demonstrated the unique advantages of QDs in the imaging of cells both in vitro and in vivo. Particularly, the recently developed near-infrared fluorescent quantum dot (NIRF-QD) with an emission range of 700-900 nm has the advantage of avoiding the interference of tissue auto fluorescence (400-600 nm). NIRF-QDs have strong tissue penetration, but do not have radiation and are not harmful in vivo. Therefore, NIRF-QDs are extremely suitable for non-invasive in vivo imaging (14,15).NIRF-QDs were conjugated with prostate specific membrane antigen monoclonal antibody (PSMA), Her2 ...

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

In vivo and in situ imaging of head and neck squamous cell carcinoma using near-infrared fluorescent quantum dot probes conjugated with epidermal growth factor receptor monoclonal antibodies in mice

et al. 2012

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“…Abbreviations: Ab, antibodies; BcacD885, human buccal squamous cell carcinoma cell line; egFr, epidermal growth factor receptor; qD800, quantum dots with a maximal emission wavelength of 800 nm. [6][7][8][9][10][11] In this study, we intravenously injected QD800-EGFR Ab probe to target EGFR, which is highly expressed by OSCC. In-vivo imaging and tumor immunohistochemical analysis showed that QD800-EGFR Ab probe can specifically bind BcaCD885 cells with high level expression of EGFR.…”

Section: Visible In-situ and In-vivo Imaging Of Oscc By Qd800-egfr Abmentioning

confidence: 99%

“…In addition, any emission spectrum from ultraviolet to near-infrared (or from blue to red) under the same excitation wavelength of light can be obtained by changing the particle size of QDs. [6][7][8] These optical characteristics of QDs are not possessed by any of the current fluorescent probes, including a variety of organic fluorescent dyes and fluorescent proteins. Particularly, the fluorescence of recently developed QDs with an emission of near-infrared wavelength from 700 nm to 900 nm has strong penetration in human tissues, which is extremely suitable for visible in-vivo medical imaging.…”

Section: Introductionmentioning

confidence: 99%

“…More importantly, these biologically functionalized QDs do not have obvious side effects in humans at the required range of detection dosage and do not affect the growth, differentiation, and function of cells. 7,8,[18][19][20] Many studies have demonstrated that 90% of OSCCs and head and neck squamous cell carcinomas (HNSCCs) highly express epidermal growth factor receptor (EGFR). 21,22 Specific targeting of EGFR by EGFR antibodies has been widely used for the treatment of OSCC and HNSCC.…”

Section: Introductionmentioning

confidence: 99%

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In-vivo imaging of oral squamous cell carcinoma by EGFR monoclonal antibody conjugated near-infrared quantum dots in mice

Yang¹

2011

IJN

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Objectives: The purpose of this study was to investigate in-vivo visible imaging of oral squamous cell carcinoma (OSCC) by targeting epidermal growth factor receptor (EGFR) with near-infrared quantum dots. Materials and methods: Quantum dots with an emission wavelength of 800 nm (QD800) were conjugated to monoclonal antibodies against EGFR, resulting in the probe designated as QD800-EGFR Ab. OSCC cell line (BcaCD885) expressing high levels of EGFR was transplanted subcutaneously into nude mice cheeks to develop an OSCC animal model. QD800-EGFR Ab containing 100 pmol equivalent of QD800 was intravenously injected into the animal model, and in-situ and in-vivo imaging of cheek squamous cell carcinoma was analyzed at 10 different time points. Results and conclusion: In-vivo imaging and immunohistochemical examination of the tumors showed that intravenously injected QD800-EGFR Ab probe could bind EGFR expressed on BcaCD885 cells. Fluorescence signals of BcaCD885 cells labeled with QD800-EGFR Ab probe could be clearly detected, and these fluorescence signals lasted for 24 hours. The most complete tumor images with maximal signal-to-noise ratio were observed from 15 minutes to 6 hours after injection of the probe. To the best of the authors’ knowledge, this is the first study that has obtained clear in-situ and in-vivo imaging of head and neck cancer by using QD800-EGFR Ab probe. The authors conclude that the combination of near-infrared quantum dots that are highly penetrating for tissues with EGFR monoclonal antibody has promising prospects in in-vivo imaging of OSCC and development of personalized surgical therapies.

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Utility and Potential Application of Nanomaterials in Medicine

Singh

Choi

Tiwari

et al. 2012

Biomedical Materials and Diagnostic Devices

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Study on effect of peptide-conjugated near-infrared fluorescent quantum dots on the clone formation, proliferation, apoptosis, and tumorigenicity ability of human buccal squamous cell carcinoma cell line BcaCD885

Cited by 20 publications

References 22 publications

In vivo and in situ imaging of head and neck squamous cell carcinoma using near-infrared fluorescent quantum dot probes conjugated with epidermal growth factor receptor monoclonal antibodies in mice

In vivo and in situ imaging of head and neck squamous cell carcinoma using near-infrared fluorescent quantum dot probes conjugated with epidermal growth factor receptor monoclonal antibodies in mice

In-vivo imaging of oral squamous cell carcinoma by EGFR monoclonal antibody conjugated near-infrared quantum dots in mice

Utility and Potential Application of Nanomaterials in Medicine

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